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Reduced hydrophobicity of the colonic mucosal surface in ulcerative colitis as a hint at a physicochemical barrier defect

Published on Aug 1, 2011in International Journal of Colorectal Disease 2.64
· DOI :10.1007/s00384-011-1190-z
Annika Braun10
Estimated H-index: 10
(University Hospital Heidelberg),
Ulrike Schönfeld1
Estimated H-index: 1
(University Hospital Heidelberg)
+ 10 AuthorsRobert Ehehalt13
Estimated H-index: 13
(University Hospital Heidelberg)
Cite
Abstract
Purpose There is increasing evidence that a defect of the gastrointestinal mucosal barrier is important for the development of inflammatory bowel diseases (IBD). The hydrophobicity of the colonic mucosal surface is a measure of its resistance to luminal antigens, e.g. of bacterial origin. Therefore, the purpose of this study was to determine this parameter in patients suffering from IBD.
  • References (35)
  • Citations (7)
Cite
References35
Newest
Published on Nov 1, 2009in Inflammatory Bowel Diseases 4.00
Annika Braun10
Estimated H-index: 10
(University Hospital Heidelberg),
Irina Treede3
Estimated H-index: 3
(University Hospital Heidelberg)
+ 11 AuthorsGerhard Erben14
Estimated H-index: 14
(DKFZ: German Cancer Research Center)
Background: Phospholipids are essential for the normal function of the intestinal mucus barrier. The objective of this study was to systematically investigate phospholipids in the intestinal mucus of humans suffering from inflammatory bowel diseases, where a barrier defect is strongly supposed to be pathogenetic. Methods: Optimal mucus recovery was first validated in healthy mice and the method was then transferred to the endoscopic acquisition of ileal and colonic mucus from 21 patients with ul...
Published on May 1, 2009in Annals of the New York Academy of Sciences 4.29
Joerg D. Schulzke12
Estimated H-index: 12
,
Svenja Ploeger1
Estimated H-index: 1
+ 7 AuthorsMichael Fromm63
Estimated H-index: 63
(Charité)
The epithelium in inflamed intestinal segments of patients with Crohn's disease is characterized by a reduction of tight junction strands, strand breaks, and alterations of tight junction protein content and composition. In ulcerative colitis, epithelial leaks appear early due to micro-erosions resulting from upregulated epithelial apoptosis and in addition to a prominent increase of claudin-2. Th1-cytokine effects by interferon-γ in combination with TNFα are important for epithelial damage in C...
Published on Jan 1, 2009in Inflammatory Bowel Diseases 4.00
Michael A. McGuckin49
Estimated H-index: 49
(UQ: University of Queensland),
Rajaraman Eri20
Estimated H-index: 20
(UQ: University of Queensland)
+ 2 AuthorsGraham L. Radford-Smith41
Estimated H-index: 41
(QIMR: QIMR Berghofer Medical Research Institute)
The etiology of human inflammatory bowel diseases (IBDs) is believed to involve inappropriate host responses to the complex commensal microbial flora in the gut, although an altered commensal flora is not completely excluded. A multifunctional cellular and secreted barrier separates the microbial flora from host tissues. Altered function of this barrier remains a major largely unexplored pathway to IBD. Although there is evidence of barrier dysfunction in IBD, it remains unclear whether this is ...
Published on Nov 6, 2007in Annals of Internal Medicine 19.32
Wolfgang Stremmel73
Estimated H-index: 73
(University Hospital Heidelberg),
Robert Ehehalt13
Estimated H-index: 13
(University Hospital Heidelberg)
+ 1 AuthorsMax Karner10
Estimated H-index: 10
(University Hospital Heidelberg)
Background: Although long-term steroid treatment is discouraged in ulcerative colitis, alternatives are lacking when therapy with immunosuppressant drugs fails. An insufficient level of phosphatidylcholine in colonic mucus is a possible pathogenetic factor for ulcerative colitis. Objective: To see whether steroid withdrawal is easier with retarded-release phosphatidylcholine or placebo in adults with chronic steroid-refractory ulcerative colitis. Design: Randomized, double-blind, placebo-control...
Published on Aug 1, 2007in Shock 3.08
Xiaofa Qin10
Estimated H-index: 10
,
Francis J. Caputo9
Estimated H-index: 9
+ 1 AuthorsEdwin A. Deitch79
Estimated H-index: 79
Published on Apr 23, 2007in International Journal of Colorectal Disease 2.64
Sebastián Videla21
Estimated H-index: 21
,
A. Lugea4
Estimated H-index: 4
+ 6 AuthorsJuan R. Malagelada57
Estimated H-index: 57
Objective Polyethylene glycol (PEG) has been suggested to protect against pathogen colonization by improving colonic barrier function. We aimed to establish whether PEG 4000 affects colonic barrier function and the development of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats.
Published on Mar 1, 2007in Gut 17.94
AlexanderSwidsinski20
Estimated H-index: 20
(Charité),
Vera Loening-Baucke12
Estimated H-index: 12
(Charité)
+ 5 AuthorsYvonne Dörffel15
Estimated H-index: 15
(Charité)
Aim: To study the role of mucus in the spatial separation of intestinal bacteria from mucosa. Patients and methods: Mucus barrier characteristics were evaluated using histological material obtained by biopsy from purged colon, colon prepared with enema and material from untreated appendices fixed with non-aqueous Carnoy solution. Bacteria were evaluated using fluorescence in situ hybridization, with bacterial 16S RNA probes and related to the periodic acid Schiff alcian blue stain. Biopsies from...
Published on Aug 1, 2006in Annals of the New York Academy of Sciences 4.29
Jan Wehkamp42
Estimated H-index: 42
(Robert Bosch Hospital),
Eduard F. Stange56
Estimated H-index: 56
(Robert Bosch Hospital)
: Crohn's disease (CD), a chronic inflammatory disease of the intestinal mucosa is usually located in the small intestine (ileum) and or in the colon. Ileal CD has been linked to a mutation in the NOD2 gene, a bacterial recognition protein. A disturbed antimicrobial defense as provided by an arsenal of different epithelial defensins seems to be a critical factor in disease pathogenesis. Defensins are antimicrobial peptides and in the ileum are mainly expressed in Paneth cells (PCs), epithelial c...
Published on Jan 1, 2006in Digestion 3.03
Francisco Guarner56
Estimated H-index: 56
The human gut is the natural habitat for a large and dynamic bacterial community. Recently developed molecular biology tools suggest that a substantial part of these bacterial populations are still to
Published on Jul 1, 2005in Gut 17.94
Wolfgang Stremmel73
Estimated H-index: 73
,
Uta Merle22
Estimated H-index: 22
+ 3 AuthorsRobert Ehehalt25
Estimated H-index: 25
Background and aims: We examined the hypothesis of an anti-inflammatory effect of phosphatidylcholine in ulcerative colitis. Methods: A phase IIA, double blind, randomised, placebo controlled study was performed in 60 patients with chronic active, non steroid dependent, ulcerative colitis, with a clinical activity index (CAI) of ⩾4. Retarded release phosphatidylcholine rich phospholipids and placebo were administered at a dose of 6 g daily over three months. The primary end point was a change in...
Cited By7
Newest
Wolfgang Stremmel73
Estimated H-index: 73
,
Simone Staffer3
Estimated H-index: 3
,
Ralf Weiskirchen49
Estimated H-index: 49
Phosphatidylcholine (PC) translocation into mucus of the intestine was shown to occur via a paracellular transport across the apical/lateral tight junction (TJ) barrier. In case this could also be operative in biliary epithelial cells, this may have implication for the pathogenesis of primary sclerosing cholangitis (PSC). We here evaluated the transport of PC across polarized cholangiocytes. Therefore, the biliary tumor cell line Mz-ChA-1 was grown to confluency. In transwell culture systems the...
Published on Oct 1, 2017in Journal of Crohns & Colitis 7.83
Wolfgang Stremmel73
Estimated H-index: 73
,
Simone Staffer3
Estimated H-index: 3
+ 8 AuthorsAlexanderSwidsinski20
Estimated H-index: 20
(Charité)
A key pathogenetic feature of ulcerative colitis [UC] is an intrinsic low mucus phosphatidylcholine[PC] content. Recently, a paracellular transport for PC across tight junctions[TJs] was described, suggesting TJ disturbance as a cause of diminished luminal PC transport. Therefore, we aimed to generate mutant mice with TJ deletion to evaluate whether a UC phenotype developed.CL57BL/6 control wild-type mice were compared to mutant mice with tamoxifen-induced villin-Cre-dependent intestinal deletio...
Published on Jul 1, 2017in Biomedical Optics Express 3.91
Hao Ding6
Estimated H-index: 6
(University of Texas Health Science Center at Houston),
Andrew W. DuPont11
Estimated H-index: 11
(University of Texas Health Science Center at Houston)
+ 4 AuthorsXiaohong Bi17
Estimated H-index: 17
(University of Texas Health Science Center at Houston)
The goal of this study is to evaluate endoscopic Raman spectroscopy as a noninvasive technique to determine histological inflammatory status of colitis. Colon mucosal composition was investigated in vivo from patients with ulcerative colitis (UC) and from age- and body mass index (BMI) matched controls using endoscope-coupled Raman spectroscopy. The results were co-registered with histological assessment of inflammatory status at the same locations. Substantial decreases (50-60%) in the content ...
Published on Jan 1, 2017
Wolfgang Stremmel73
Estimated H-index: 73
,
Simone Staffer3
Estimated H-index: 3
+ 4 AuthorsDaniel Hornuss1
Estimated H-index: 1
Background: Attack by commensal microbiota is one component of induction of inflammatory episodes in ulcerative colitis (UC). In UC, the mucus layer is intrinsica
Published on Sep 1, 2016in Biochimica et Biophysica Acta 3.79
Wolfgang Stremmel73
Estimated H-index: 73
,
Simone Staffer3
Estimated H-index: 3
+ 3 AuthorsAnnika Gauss7
Estimated H-index: 7
Abstract Phosphatidylcholine (PC) is the most abundant phospholipid in intestinal mucus, indicative of a specific transport system across the mucosal epithelium to the intestinal lumen. To elucidate this transport mechanism, we employed a transwell tissue culture system with polarized CaCo2 cells. It was shown that PC could not substantially be internalized by the cells. However, after basal application of increasing PC concentrations, an apical transport of 47.1 ± 6.3 nmol h − 1 mM PC − 1 was o...
Published on Jul 1, 2014in The American Journal of Gastroenterology 10.24
Max Karner10
Estimated H-index: 10
,
Andreas Kocjan2
Estimated H-index: 2
+ 9 AuthorsFrank Zuelch2
Estimated H-index: 2
OBJECTIVES: Phosphatidylcholine is a key component of the mucosal barrier. Treatment with modified release phosphatidylcholine aims to improve the impaired barrier function. The primary objective is to evaluate the efficacy of LT-02, a newly designed modified release phosphatidylcholine formula, in a multicenter setting. METHODS: This is a double-blinded, randomized, placebo-controlled, superiority study conducted in 24 ambulatory referral centers in Germany, Lithuania, and Romania. A total of 1...
Published on Jan 1, 2013in Digestive Diseases 2.91
Wolfgang Stremmel73
Estimated H-index: 73
,
Annika Gauss7
Estimated H-index: 7
Lecithin [phosphatidylcholine (PC)] was shown to account for more than 70% of total phospholipids within the intestinal mucus layer. It is arranged in lamellar membranes (surfactant-like particles) an
Published on Jan 1, 2012in Digestive Diseases 2.91
Wolfgang Stremmel73
Estimated H-index: 73
,
Robert Ehehalt25
Estimated H-index: 25
+ 4 AuthorsAnnika Braun10
Estimated H-index: 10
The colonic mucus serves a first barrier towards invasion of commensal bacteria in stools to prevent inflammation. One essential component of intestinal mucus is phosphatidylcholine (PC) which represe