Residue frequencies and pairing preferences at protein-protein interfaces

We used a nonredundant set of 621 protein–protein interfaces of known high-resolution structure to derive residue composition and residue–residue contact preferences. The residue composition at the interfaces, in entire proteins and in whole genomes correlates well, indicating the statistical strength of the data set. Differences between amino acid distributions were observed for interfaces with buried surface area of less than 1,000 Å2 versus...