SIMULTANEOUS DETERMINATION OF THREE BIOACTIVE INGREDIENTS OF DA CHUANXIONG FANG IN RAT PLASMA BY HPLC-DAD: APPLICATION TO PHARMACOKINETIC STUDY

A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous determination of gastrodin (1), ferulic acid (2), and senkyunolide I (3) in rat plasma after oral administration of a famous traditional Chinese medicine, that is, Da ChuanXiong Fang. The analysis was performed on a reversed-phase C18 column with a gradient eluent composed of acetonitrile and 1% acetic acid. The flow rate was kept at 1 mL/min...