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The role of purification in the crystallization of proteins and nucleic acids

Published on Aug 1, 1986in Journal of Crystal Growth1.57
· DOI :10.1016/0022-0248(86)90172-7
Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique),
Anne-Catherine Dock5
Estimated H-index: 5
(CNRS: Centre national de la recherche scientifique)
+ 3 AuthorsDino Moras79
Estimated H-index: 79
(CNRS: Centre national de la recherche scientifique)
Abstract
Abstract In structural biology, the crystallization of the macromolecules often represents the most challenging step. Beside classical factors which determine the solubility of macromolecules, purity of compounds is another major parameter governing crystal growth. With aminoacyl-tRNA synthetases and transfer ribonucleic acids as examples, it will be shown that molecules to be crystallized not only have to be pure in terms of contaminating molecules, but also in terms of sequence integrity and conformational homogeneity. A chromatographic method based on salting-out of proteins or nucleic acids on Sepharose 4B gels and back-solubilization with inverse salt gradients will be discussed in the light of crystal growth experiments.
  • References (42)
  • Citations (70)
References42
Newest
#1K. GulewiczH-Index: 1
#2D. AdamiakH-Index: 1
Last.Mathias Sprinzl (University of Bayreuth)H-Index: 32
view all 3 authors...
#1I. Amiri (CNRS: Centre national de la recherche scientifique)H-Index: 3
#2Hafedh Mejdoub (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last.Joseph Reinbolt (CNRS: Centre national de la recherche scientifique)H-Index: 20
view all 5 authors...
#1Bernard Colas (CNRS: Centre national de la recherche scientifique)H-Index: 13
#2Yves Boulanger (CNRS: Centre national de la recherche scientifique)H-Index: 16
Cited By70
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#1Arayik Martirosyan (UHH: University of Hamburg)
#2Lawrence J. DeLucas (The Aerospace Corporation)
Last.Christian Betzel (UHH: University of Hamburg)H-Index: 38
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#1Catherine Florentz (UDS: University of Strasbourg)H-Index: 42
#2Richard Giegé (UDS: University of Strasbourg)H-Index: 53
#1Jiong Chen (HAUST: Henan University of Science and Technology)H-Index: 1
#2Wei Feng (HAUST: Henan University of Science and Technology)H-Index: 1
Last.Yue ZhaoH-Index: 1
view all 3 authors...
#1Mark L. Brader (University of Auckland)H-Index: 1
#2Edward N. Baker (University of Auckland)H-Index: 63
Last.John F. Carpenter (Anschutz Medical Campus)H-Index: 69
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View next paperPreparation and analysis of protein crystals