Phase extension and refinement of Bence–Jones protein Rhe (1.9 Å)

A procedure is described whereby crude atomic coordinates obtained from a medium-resolution electron density map (~3-4 Å) with the aid of Watson-Kendrew models may be refined to native data of near-atomic resolution using only a limited subset of the data and non-interactive computer graphics. This refinement procedure for the Bence-Jones protein Rhe included phase extension from 3.0 to 1.9 Å and led to an improved crystallographic sequence for...