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Determination of Dexamethasone in Bovine Liver by Chemiluminescence High-Performance Liquid Chromatography
Published on Oct 1, 1999in Journal of Agricultural and Food Chemistry 3.41
· DOI :10.1021/jf990334b
Y. Iglesias3
Estimated H-index: 3
,
Cristina A. Fente26
Estimated H-index: 26
+ 4 AuthorsP.G Gigosos3
Estimated H-index: 3
Abstract
A new method for the determination of dexamethasone (9α-fluoro-11β,17α,21-trihydroxy-16α-methylpregna-1,4-diene-3,20-dione) in bovine liver was developed. This new liquid−liquid extraction method comprises the addition of sodium hydroxide to the tissue sample followed by extraction with ethyl acetate. After centrifugation, the extract is evaporated to dryness and the residue dissolved in acetonitrile. The cleaning of the fat is performed with n-hexane, and the acetonitrile layer is evaporated. Analysis of the extracts is performed using high-performance liquid chromatography with chemiluminescence detection employing luminol as CL reagent. A series of recovery curves performed at spiking levels of 50, 30, 10, 5, and 2.5 ppb show that at least 80% of DEX can be recovered from liver and that the chemiluminescence detection yields satisfactory results with respect to sensitivity (LOD 0.2 ppb), reproducibility (CV% 10.7) and repeatability (CV% 6.2−8.9). Keywords: HPLC; chemiluminescence; dexamethasone; liver;...
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Cited By15
Alireza Khataee48
Estimated H-index: 48
(University of Tabriz),
Aliyeh Hasanzadeh8
Estimated H-index: 8
(University of Tabriz)
+ 2 AuthorsSang Woo Joo29
Estimated H-index: 29
(Yeungnam University)
Abstract l -Cysteine capped CdS quantum dots (QDs) were synthesized through a facile hydrothermal method and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), photoluminescence (PL) and UV–Vis spectroscopy. The light emitted from KMnO 4 – l -cysteine capped CdS QDs reaction in acidic medium was applied as a simple and sensitive chemiluminescence (CL) system for determination of dexamethasone. The CL intensity of KMnO 4 – l -cysteine capped CdS QDs CL system was remark...
8 Citations Source Cite
Published on Aug 1, 2012in Food Analytical Methods 2.25
Hanwen Sun12
Estimated H-index: 12
(Hebei University),
Zhansheng Kang1
Estimated H-index: 1
(Hebei University)
+ 2 AuthorsYunkai Lv12
Estimated H-index: 12
(Hebei University)
A new method for multi-residue determination of five synthetic glucocorticoids (betamethasone, dexamethasone, prednisone, prednisolone, and hydrocortisone) in milk powder is developed. The glucocorticoids in the samples were extracted with tert-butyl methyl ether under ultrasonication incubation, and then cleaned up through gel permeation chromatography. After C18 LC gradient elution separation using acetonitrile with 0.1% formic acid as a mobile phase, the eluents were determined by liquid chro...
3 Citations Source Cite
Published on Sep 1, 2002in Analytica Chimica Acta 5.12
Y. Iglesias3
Estimated H-index: 3
(University of Santiago de Compostela),
Cristina A. Fente26
Estimated H-index: 26
(University of Santiago de Compostela)
+ 3 AuthorsS. Mayo3
Estimated H-index: 3
(Xunta de Galicia)
Abstract A luminol chemiluminescence (CL) reaction was applied for the simultaneous determination of nine corticosteroids (triamcinolone, prednisolone, prednisone, cortisone, betamethasone, dexamethasone, corticosterone, flumethasone and triamcinolone acetonide) using flow injection. The luminol solution was prepared in NaOH with hexacyanoferrate(II) and hexacyanoferrate(III) was used as oxidant. Adequate isocratic chromatographic separation was obtained using a simple mobile phase (acetonitrile...
17 Citations Source Cite
Published on Jan 1, 2010in Biological & Pharmaceutical Bulletin 1.69
Masayuki Kohno12
Estimated H-index: 12
(Kyoto University),
Mari Haramoto6
Estimated H-index: 6
(Kyoto University)
+ 5 AuthorsKoji Kawakami26
Estimated H-index: 26
(Kyoto University)
Corticosteroids are routinely used in patients with pulmonary fibrosis, yet they have several adverse effects. To improve this situation, we used an animal model of pulmonary injury and early fibrosis and investigated whether the combination of an intrapulmonary inhalation device with antedrug budesonide (BUD) administered to the lung had greater efficacy and fewer systemic adverse effects compared to long-acting dexamethasone (DEX). BUD or DEX was administrated either intrapulmonary or intraven...
7 Citations Source Cite
Published on Jan 1, 2000in Analyst 3.86
Y. Iglesias3
Estimated H-index: 3
(University of Santiago de Compostela),
Cristina A. Fente26
Estimated H-index: 26
(University of Santiago de Compostela)
+ 3 AuthorsAlberto Cepeda31
Estimated H-index: 31
(University of Santiago de Compostela)
A method for the determination of nine corticosteroids in bovine liver was developed. The liquid–liquid extraction method consisted in the addition of sodium hydroxide to the tissue sample followed by extraction with ethyl acetate. After centrifugation, the extract was evaporated to dryness and the residue redissolved in acetonitrile. The removal of fats was performed with n-hexane and the acetonitrile layer was evaporated. Afterwards, analysis of the extracts was performed using high-performanc...
14 Citations Source Cite
Published on Nov 1, 2002in Analytica Chimica Acta 5.12
O. van den Hauwe3
Estimated H-index: 3
(Ghent University),
Frédéric Dumoulin8
Estimated H-index: 8
(Ghent University)
+ 3 AuthorsC. Van Peteghem30
Estimated H-index: 30
(Ghent University)
Abstract A liquid chromatography combined to mass spectrometry (LC–MS/MS) method to confirm the presence of 11 synthetic glucocorticoids in cattle liver is described. After an enzymatic deconjugation step, liver samples were treated with methanol to extract the glucocorticoids. A further cleanup of the extracts was carried out by SPE. LC separations were carried out as described previously using a column packed with porous graphitic carbon. For the detection positive (ESI+) and negative (ESI−) e...
60 Citations Source Cite
Dongmei Chen25
Estimated H-index: 25
(Huazhong Agricultural University),
Yanfei Tao25
Estimated H-index: 25
(Huazhong Agricultural University)
+ 4 AuthorsZonghui Yuan33
Estimated H-index: 33
(Huazhong Agricultural University)
A confirmatory and quantitative method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine the presence of eight glucocorticoids (prednisone, prednisolone, hydrocortisone, methylprednisolone, dexamethasone, betamethasone, beclomethasone, and fludrocortisone) in the muscles and livers of swine, cattle, and sheep and the muscle of chicken is described. After deconjugation in alkali media, samples were extracted with ethyl acetate for glucocorticoids followed by solid-phase...
21 Citations Source Cite
Published on Jan 1, 2011in Journal of Chromatography B 2.44
Dongmei Chen25
Estimated H-index: 25
(Huazhong Agricultural University),
Yanfei Tao25
Estimated H-index: 25
(Huazhong Agricultural University)
+ 9 AuthorsMenghong Dai22
Estimated H-index: 22
(Huazhong Agricultural University)
Abstract A multi-residues method using pressurized liquid extraction (PLE) and liquid chromatography combined with mass spectrometry (LC–MS/MS) has been developed for determination of eight glucocorticoids (prednisone, prednisolone, hydrocortisone, methylprednisolone, dexamethasone, betamethasone, beclomethasone, fludrocortisone) in muscle of swine, cattle, and sheep. Parameters affecting PLE extraction including extraction solvent, extraction temperature, extraction pressure and extraction cycl...
25 Citations Source Cite
Published on Jun 15, 2001in Rapid Communications in Mass Spectrometry 1.97
O. van den Hauwe3
Estimated H-index: 3
(Ghent University),
J. Castro Perez1
Estimated H-index: 1
+ 1 AuthorsC. Van Peteghem30
Estimated H-index: 30
(Ghent University)
In this study a new method for the simultaneous confirmation of betamethasone and dexamethasone residues in bovine liver is presented. A Quattro LCZ triple quadrupole mass spectrometer, equipped with an atmospheric pressure ionization (API) source, was coupled to a high performance liquid chromatograph (HPLC) system. Spiked liver samples were first extracted with acetonitrile, and the extracts were purified on C-18 columns. LC separations were performed on a Hypercarb column, with acetonitrile/w...
34 Citations Source Cite
Published on Dec 1, 2009in Bioanalytical Reviews
Makoto Tsunoda24
Estimated H-index: 24
(University of Tokyo)
Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis. This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemi...
5 Citations Source Cite
View next paperDetermination of Dexamethasone in Liver and Muscle by Liquid Chromatography and Gas Chromatography/Mass Spectrometry