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Determination of Dexamethasone in Bovine Liver by Chemiluminescence High-Performance Liquid Chromatography

Published on Oct 1, 1999in Journal of Agricultural and Food Chemistry 3.41
· DOI :10.1021/jf990334b
Y. Iglesias3
Estimated H-index: 3
,
C. Fente26
Estimated H-index: 26
+ 4 AuthorsP.G Gigosos3
Estimated H-index: 3
Abstract
A new method for the determination of dexamethasone (9α-fluoro-11β,17α,21-trihydroxy-16α-methylpregna-1,4-diene-3,20-dione) in bovine liver was developed. This new liquid−liquid extraction method comprises the addition of sodium hydroxide to the tissue sample followed by extraction with ethyl acetate. After centrifugation, the extract is evaporated to dryness and the residue dissolved in acetonitrile. The cleaning of the fat is performed with n-hexane, and the acetonitrile layer is evaporated. Analysis of the extracts is performed using high-performance liquid chromatography with chemiluminescence detection employing luminol as CL reagent. A series of recovery curves performed at spiking levels of 50, 30, 10, 5, and 2.5 ppb show that at least 80% of DEX can be recovered from liver and that the chemiluminescence detection yields satisfactory results with respect to sensitivity (LOD 0.2 ppb), reproducibility (CV% 10.7) and repeatability (CV% 6.2−8.9). Keywords: HPLC; chemiluminescence; dexamethasone; liver;...
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Alireza Khataee49
Estimated H-index: 49
(University of Tabriz),
Aliyeh Hasanzadeh8
Estimated H-index: 8
(University of Tabriz)
+ 2 AuthorsSang Woo Joo28
Estimated H-index: 28
(Yeungnam University)
Abstract l -Cysteine capped CdS quantum dots (QDs) were synthesized through a facile hydrothermal method and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), photoluminescence (PL) and UV–Vis spectroscopy. The light emitted from KMnO 4 – l -cysteine capped CdS QDs reaction in acidic medium was applied as a simple and sensitive chemiluminescence (CL) system for determination of dexamethasone. The CL intensity of KMnO 4 – l -cysteine capped CdS QDs CL system was remark...
8 Citations Source Cite
Published on Aug 1, 2012in Food Analytical Methods 2.25
Hanwen Sun20
Estimated H-index: 20
(Hebei University),
Zhansheng Kang1
Estimated H-index: 1
(Hebei University)
+ 2 AuthorsYunkai Lv13
Estimated H-index: 13
(Hebei University)
A new method for multi-residue determination of five synthetic glucocorticoids (betamethasone, dexamethasone, prednisone, prednisolone, and hydrocortisone) in milk powder is developed. The glucocorticoids in the samples were extracted with tert-butyl methyl ether under ultrasonication incubation, and then cleaned up through gel permeation chromatography. After C18 LC gradient elution separation using acetonitrile with 0.1% formic acid as a mobile phase, the eluents were determined by liquid chro...
3 Citations Source Cite
Published on Jan 1, 2012
Hanwen Sun20
Estimated H-index: 20
,
Zhansheng Kang1
Estimated H-index: 1
+ 2 AuthorsYunkai Lv13
Estimated H-index: 13
A new method for multi-residue determination of five synthetic glucocorticoids (betamethasone, dexametha- sone, prednisone, prednisolone, and hydrocortisone) in milk powder is developed. The glucocorticoids in the samples were extracted with tert-butyl methyl ether under ultra- sonication incubation, and then cleaned up through gel permeation chromatography. After C18 LC gradient elution separation using acetonitrile with 0.1% formic acid as a mobile phase, the eluents were determined by liquid ...
Published on Jan 1, 2011in Journal of Chromatography B 2.44
Dongmei Chen27
Estimated H-index: 27
(Huazhong Agricultural University),
Yanfei Tao26
Estimated H-index: 26
(Huazhong Agricultural University)
+ 9 AuthorsMenghong Dai23
Estimated H-index: 23
(Huazhong Agricultural University)
Abstract A multi-residues method using pressurized liquid extraction (PLE) and liquid chromatography combined with mass spectrometry (LC–MS/MS) has been developed for determination of eight glucocorticoids (prednisone, prednisolone, hydrocortisone, methylprednisolone, dexamethasone, betamethasone, beclomethasone, fludrocortisone) in muscle of swine, cattle, and sheep. Parameters affecting PLE extraction including extraction solvent, extraction temperature, extraction pressure and extraction cycl...
27 Citations Source Cite
Dongmei Chen27
Estimated H-index: 27
(Huazhong Agricultural University),
Yanfei Tao26
Estimated H-index: 26
(Huazhong Agricultural University)
+ 4 AuthorsZonghui Yuan34
Estimated H-index: 34
(Huazhong Agricultural University)
A confirmatory and quantitative method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine the presence of eight glucocorticoids (prednisone, prednisolone, hydrocortisone, methylprednisolone, dexamethasone, betamethasone, beclomethasone, and fludrocortisone) in the muscles and livers of swine, cattle, and sheep and the muscle of chicken is described. After deconjugation in alkali media, samples were extracted with ethyl acetate for glucocorticoids followed by solid-phase...
22 Citations Source Cite
Published on Jan 1, 2010in Biological & Pharmaceutical Bulletin 1.69
Masayuki Kohno12
Estimated H-index: 12
(Kyoto University),
Mari Haramoto5
Estimated H-index: 5
(Kyoto University)
+ 5 AuthorsKoji Kawakami28
Estimated H-index: 28
(Kyoto University)
Corticosteroids are routinely used in patients with pulmonary fibrosis, yet they have several adverse effects. To improve this situation, we used an animal model of pulmonary injury and early fibrosis and investigated whether the combination of an intrapulmonary inhalation device with antedrug budesonide (BUD) administered to the lung had greater efficacy and fewer systemic adverse effects compared to long-acting dexamethasone (DEX). BUD or DEX was administrated either intrapulmonary or intraven...
7 Citations Source Cite
Published on Dec 1, 2009in Bioanalytical Reviews
Makoto Tsunoda24
Estimated H-index: 24
(University of Tokyo)
Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis. This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemi...
5 Citations Source Cite
Published on Jun 1, 2007in Journal of Chromatography A 3.72
Yantu Zhang7
Estimated H-index: 7
(Shaanxi Normal University),
Zhujun Zhang30
Estimated H-index: 30
(Shaanxi Normal University)
+ 1 AuthorsYue Wei6
Estimated H-index: 6
(Shaanxi Normal University)
Abstract A novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (TR), prednisolone (PR), hydrocortisone (HC), cortisone (CO), methylprednisolone (MP), dexamethasone (DE) and triamcinolone acetonide (TA) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the enhancement effect of glucocorticoids on the chemiluminescence reaction between luminol and the complex of triva...
17 Citations Source Cite
Published on May 1, 2005in Luminescence 1.67
Beatriz I. Vázquez25
Estimated H-index: 25
(University of Santiago de Compostela),
Xesús Feás19
Estimated H-index: 19
(University of Santiago de Compostela)
+ 3 AuthorsA. Cepeda32
Estimated H-index: 32
(University of Santiago de Compostela)
The development of a black market of chemical cocktails for illegal growth promotion in food-producing animals includes substances that are potentially dangerous for human health, such as synthetic corticosteroids. The potential presence of these residues in food makes it necessary to develop rapid and sensitive analytical methodologies to detect such substances, preferably in live animals before they arrive at the market. A chemiluminescence (CL) detection method for the determination of four s...
15 Citations Source Cite
Published on Mar 1, 2003in Biomedical Chromatography 1.69
Famei Li24
Estimated H-index: 24
(Shenyang Pharmaceutical University),
Caihua Zhang1
Estimated H-index: 1
(Shenyang Pharmaceutical University)
+ 1 AuthorsWanyu Feng1
Estimated H-index: 1
(China Medical University (PRC))
The present paper reviews the developments and applications of chemiluminescence detection with HPLC and CE in pharmaceutical and biomedical analysis. The chemiluminescence systems, chemiluminogenic reagents and derivatization reagents, improvements in instrumental design as well as their contributions to the practical applications, are all presented. The advantages and limitations of current detection methodology and future prospects for improvement are briefly discussed. Copyright © 2003 John ...
37 Citations Source Cite