Sperm head morphology and nuclear chromatin structure evaluated by flow cytometry in a diallel cross in mice

Genetic factors affecting spermatogenesis, sperm morphology, and chromatin structure in mice were estimated using a diallel cross of the inbred lines C3H/HeJ, C57BL/6J, DBA/2J, and BALB/cByJ. Flow cytometry of acridine orange stained cells was used to evaluate (i) proportions of testicular tetraploid, diploid, and haploid cells and (ii) nuclear chromatin structure of sperm, measured by resistance of chromatin to in situ acid denaturation, and...