A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
Abstract
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian expression vectors of multiple DNA fragments and with maximum flexibility, both for choice of vector backbone and cargo. The vector system includes a set of basic vectors and a toolbox containing...
Paper Details
Title
A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
Published Date
May 30, 2014
Journal
Volume
9
Issue
5
Pages
e96693 - e96693
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