Synthesis of multiple gene products from a single polycistronic mRNA using the Thermococcus kodakaraensis-based translation system

Previously, we have developed a system for cell-free protein synthesis operated at high temperatures using a lysate of thermococcus kodakaraensis. Here, we tested the synthesis of multiple gene products from a single mRNA. The selected target proteins were TK0944 and TK0943 proteins, the alpha-subunit and the szlig-subunit of acetyl-CoA synthetase III (ACS III) forming an intracellular enzyme complex composed of two beta- and two szlig-subunits...