A manual nanoscale method for protein crystallization

To overcome one of the major hurdles in three-dimensional crystal structure determination – the requirement for large quantities of purified material to grow crystals – crystallization methodologies have been developed that require only a total of 2–5 µl of a concentrated macromolecular solution to screen more than 100 conditions. These procedures employ a circular slide containing an array of 25 wells designed for crystallization setups in the...