UPLC-ESI-MS-MS Determination of Three β2-Agonists in Pork

Matrix-induced ion suppression and different polarity usually interfere with extraction, purification and drug analysis, therefore a method was developed for determination of three β2-agonists namely, salbutamol, ractopamine and clenbuterol in pork. After enzymatic hydrolysis, a 2.0 g homogenized pork sample was extracted, with perchloric acid–methanol (9:1, v/v) to precipitate protein, and then cleaned by mixed-mode Bond Elut Plexa PCX solid...