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The Effect of Tetramethylpyrazine on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells

Published on Oct 1, 2009in Basic & Clinical Pharmacology & Toxicology2.45
· DOI :10.1111/j.1742-7843.2009.00470.x
Wen-Ming Li9
Estimated H-index: 9
(CQMU: Chongqing Medical University),
Hongtao Liu17
Estimated H-index: 17
(CQMU: Chongqing Medical University)
+ 5 AuthorsChao Yu17
Estimated H-index: 17
(CQMU: Chongqing Medical University)
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Abstract
Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H(2)O(2)-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H(2)O(2)-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H(2)O(2) for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H(2)O(2)-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H(2)O(2)-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H(2)O(2)-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H(2)O(2)-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H(2)O(2)-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
  • References (37)
  • Citations (35)
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References37
Newest
#1Hongtao Liu (CAS: Chinese Academy of Sciences)H-Index: 17
#2Wen-Ming Li (CQMU: Chongqing Medical University)H-Index: 9
Last.Yuguang Du (DICP: Dalian Institute of Chemical Physics)H-Index: 31
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#1Yu-Show Fu (NYMU: National Yang-Ming University)H-Index: 13
#2Yen Yang Lin (NYMU: National Yang-Ming University)H-Index: 1
Last.Yu Yi Fu (Nan Kai University of Technology)H-Index: 1
view all 10 authors...
#1Ye-Ming Liu (DUT: Dalian University of Technology)H-Index: 1
#2Bo Jiang (DUT: Dalian University of Technology)H-Index: 19
Last.Lijia An (DUT: Dalian University of Technology)H-Index: 16
view all 4 authors...
#1Chang-suo Liu (Peking Union Medical College)H-Index: 2
#2Nai-Hong Chen (Peking Union Medical College)H-Index: 26
Last.Jun-tian Zhang (Peking Union Medical College)H-Index: 7
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#1Xin-Rui Cheng (Peking Union Medical College)H-Index: 3
#2Li Zhang (Peking Union Medical College)H-Index: 16
Last.Guanhua Du (Peking Union Medical College)H-Index: 39
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#1Eresha Mendis (Pukyong National University)H-Index: 19
#2Moon-Moo Kim (Pukyong National University)H-Index: 28
Last.Se-Kwon Kim (Pukyong National University)H-Index: 82
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Cited By35
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#1Wen-Tao Sun (TEDA International Cardiovascular Hospital)
#2Xiang-Chong Wang (Hebei University)
Last.Qin Yang (TEDA International Cardiovascular Hospital)H-Index: 1
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#1Wenjie Jian (SCAU: South China Agricultural University)H-Index: 4
#2Liying Tu (JMU: Jimei University)H-Index: 1
Last.Yuanming Sun (SCAU: South China Agricultural University)H-Index: 20
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#1Joshua Hahn (University of Alberta Hospital)H-Index: 1
#2Leila Laouar (University of Alberta Hospital)H-Index: 2
Last.Nadr M. Jomha (University of Alberta Hospital)H-Index: 9
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#1Cheng Yang (SYSU: Sun Yat-sen University)H-Index: 4
#2Yue Xu (SYSU: Sun Yat-sen University)H-Index: 7
Last.Xiaoling Liang (SYSU: Sun Yat-sen University)H-Index: 5
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