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The Effect of Tetramethylpyrazine on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells

Published on Oct 1, 2009in Basic & Clinical Pharmacology & Toxicology 2.45
· DOI :10.1111/j.1742-7843.2009.00470.x
Wen-Ming Li9
Estimated H-index: 9
(CQMU: Chongqing Medical University),
Hongtao Liu17
Estimated H-index: 17
(CQMU: Chongqing Medical University)
+ 5 AuthorsChao Yu17
Estimated H-index: 17
(CQMU: Chongqing Medical University)
Cite
Abstract
Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H(2)O(2)-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H(2)O(2)-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H(2)O(2) for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H(2)O(2)-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H(2)O(2)-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H(2)O(2)-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H(2)O(2)-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H(2)O(2)-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
  • References (37)
  • Citations (35)
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References37
Newest
Published on Mar 1, 2009in Pharmacological Research 5.57
Hongtao Liu17
Estimated H-index: 17
(CAS: Chinese Academy of Sciences),
Wen-Ming Li9
Estimated H-index: 9
(CQMU: Chongqing Medical University)
+ 5 AuthorsYuguang Du31
Estimated H-index: 31
(DICP: Dalian Institute of Chemical Physics)
Abstract Chitosan oligosaccharides (COS) have been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we investigated the protective effects of COS against hydrogen peroxide (H 2 O 2 )-induced oxidative damage on human umbilical vein endothelial cells (HUVEC, ECV304 cells). After 24 h pre-incubation with COS (25–200 μg/ml), the viability loss in ECV304 cells induced by H 2 O 2 (300 μM) for 12 h was m...
110 Citations Source Cite
Published on Apr 1, 2008in Biochemical and Biophysical Research Communications 2.71
Tetsuya Moriue12
Estimated H-index: 12
(Kagawa University),
Junsuke Igarashi17
Estimated H-index: 17
(Kagawa University)
+ 3 AuthorsYasuo Kubota24
Estimated H-index: 24
(Kagawa University)
Abstract Reactive oxygen species including H 2 O 2 lead vascular endothelial cells (EC) to undergo apoptosis. Sphingosine 1-phosphate (S1P) is a platelet-derived sphingolipid mediator that elicits various EC responses. We aimed to explore whether and how S1P modulates EC apoptosis induced by H 2 O 2 . Treatment of cultured bovine aortic EC (BAEC) with H 2 O 2 (750 μM for 6 h) led to DNA fragmentation (ELISA), DNA nick formation (TUNEL staining), and cleavage of caspase-3, key features of EC apop...
33 Citations Source Cite
Published on Apr 1, 2008in Neuro-oncology 10.09
Yu-Show Fu13
Estimated H-index: 13
(NYMU: National Yang-Ming University),
Yen Yang Lin1
Estimated H-index: 1
(NYMU: National Yang-Ming University)
+ 8 AuthorsYu Yi Fu1
Estimated H-index: 1
(Nan Kai University of Technology)
We tested the herbal extract 2,3,5,6-tetramethylpyrazine (TMP) for possible therapeutic efficacy against a glioma cell line and against gliomas transplanted into rat brains. In the cultured glioma cells, 50 μM TMP significantly inhibited glutamate-induced increase in intracellular calcium. Significant cell damage (30%) and proliferation suppression (10%), however, occurred only at higher concentrations (200–400 μM). Glioma- neuronal co-culturing resulted in significant neuronal damage and higher...
30 Citations Source Cite
Published on Mar 1, 2008in Toxicology in Vitro 3.07
Ye-Ming Liu1
Estimated H-index: 1
(DUT: Dalian University of Technology),
Bo Jiang19
Estimated H-index: 19
(DUT: Dalian University of Technology)
+ 1 AuthorsLijia An16
Estimated H-index: 16
(DUT: Dalian University of Technology)
Protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia (A.) oxyphylla, showed the significant neuroprotective effects on hydrogen peroxide (H2O2) or MPP+-induced apoptosis in cultured PC12 cells. However, the mechanism how PCA suppresses rotenone-induced neurotoxicity in cultured PC12 cells remains unclear. In this study, we investigated the protective effects of PCA in PC12 cells exposed to rotenone as an in vitro model of mitochondrial dysfunction and apoptotic cel...
43 Citations Source Cite
Published on Oct 1, 2007in Anesthesia & Analgesia 3.49
Baohua Wang6
Estimated H-index: 6
,
Tao Luo2
Estimated H-index: 2
+ 1 AuthorsDavid M. Ansley19
Estimated H-index: 19
BACKGROUND:Vascular endothelial cells play an important role in maintaining cardiovascular homeostasis. Oxidative stress is a critical pathogenic factor in endothelial cell damage and the development of cardiovascular diseases. In this study we evaluated the effects of propofol on oxidative stress-i
49 Citations Source Cite
Published on Aug 1, 2007in Phytomedicine 4.18
Chang-suo Liu2
Estimated H-index: 2
(Peking Union Medical College),
Nai-Hong Chen26
Estimated H-index: 26
(Peking Union Medical College),
Jun-tian Zhang7
Estimated H-index: 7
(Peking Union Medical College)
Abstract A number of studies indicate that reactive oxygen species (ROS) are involved in neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The neuroprotective effects of salvianolic acid B (SalB) from Radix Salviae miltiorrhizae (RSM) against hydrogen peroxide (H 2 O 2 )-induced rat pheochromocytoma line PC12 injury were evaluated in the present study. Vitamin E, a potent antioxidant, was employed as a positive control agent. Following exposure of cells t...
71 Citations Source Cite
Published on May 1, 2007in Cell Biology International 2.13
Xin-Rui Cheng3
Estimated H-index: 3
(Peking Union Medical College),
Li Zhang16
Estimated H-index: 16
(Peking Union Medical College)
+ 2 AuthorsGuanhua Du39
Estimated H-index: 39
(Peking Union Medical College)
In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only suppressed the down-regulation of Bcl-2, up-regulation of Bax and the release of mitochondrial cytochrome c to cytosol, but also attenuated caspase-3 activation and eventually prot...
66 Citations Source Cite
Published on May 1, 2007in Life Sciences 3.45
Eresha Mendis19
Estimated H-index: 19
(Pukyong National University),
Moon-Moo Kim28
Estimated H-index: 28
(Pukyong National University)
+ 1 AuthorsSe-Kwon Kim82
Estimated H-index: 82
(Pukyong National University)
Abstract Despite extensive study on biological activities of chitosan and chitooligosaccharides (COS), there is no experimental evidence available as to COS mediated inhibition of free radical damage in cellular oxidizing systems. In this study, radical scavenging efficacies of different molecular weight bearing COS were assessed and their intracellular radical scavenging effects were tested employing B16F1, murine melanoma cell line. The results exhibited appreciable suppression in occurrence o...
83 Citations Source Cite
Dang Sc1
Estimated H-index: 1
,
Jie Zhang10
Estimated H-index: 10
+ 2 AuthorsFan X1
Estimated H-index: 1
BACKGROUND: Acute necrotizing pancreatitis (ANP) leads to a systemic inflammatory response characterized by widespread leukocyte activation and, as a consequence, distant organ injury. The aim of this study was to explore the relationship between gastric microcirculatory impairment and inflammatory mediators released in rats and to evaluate the therapeutic effect of ligustrazine extracted from Rhizoma ligusticum wallichii on gastric mucosa injury in a rat model of ANP. METHODS: Ninety-six Spragu...
16 Citations
Micaela Iantorno18
Estimated H-index: 18
,
Hui Chen33
Estimated H-index: 33
+ 4 AuthorsMichael J. Quon74
Estimated H-index: 74
Ghrelin is an orexigenic peptide hormone secreted by the stomach. In patients with metabolic syndrome and low ghrelin levels, intra-arterial ghrelin administration acutely improves their endothelial dysfunction. Therefore, we hypothesized that ghrelin activates endothelial nitric oxide synthase (eNOS) in vascular endothelium, resulting in increased production of nitric oxide (NO) using signaling pathways shared in common with the insulin receptor. Similar to insulin, ghrelin acutely stimulated i...
84 Citations Source Cite
Cited By35
Newest
Published on Feb 1, 2019in Vascular Pharmacology 3.33
Wen-Tao Sun (TEDA International Cardiovascular Hospital), Xiang-Chong Wang (Hebei University)+ 3 AuthorsQin Yang1
Estimated H-index: 1
(TEDA International Cardiovascular Hospital)
Abstract Objectives We recently reported the involvement of ER stress-mediated BK Ca channel inhibition in homocysteine-induced coronary dilator dysfunction. In another study, we demonstrated that tetramethylpyrazine (TMP), an active ingredient of the Chinese herb Chuanxiong, possesses potent anti-ER stress capacity. The present study investigated whether TMP protects BK Ca channels from homocysteine-induced inhibition and whether suppression of ER stress is a mechanism contributing to the prote...
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Published on Dec 1, 2018in Poultry Science 2.03
Xiaolong Qi4
Estimated H-index: 4
(H.I., S.I.: University of Agriculture, Faisalabad),
Jing Wang5
Estimated H-index: 5
+ 6 AuthorsG. H. Qi11
Estimated H-index: 11
1 Citations Source Cite
Published on Aug 1, 2018in Journal of International Medical Research 1.35
Yutao Liu1
Estimated H-index: 1
,
Xu Li1
Estimated H-index: 1
+ 1 AuthorsQuanli Ge1
Estimated H-index: 1
ObjectivesHigh glucose-induced alterations in vascular smooth muscle cell behavior have not been fully characterized. We explored the protective mechanism of tetramethylpyrazine (TMP) on rat smooth muscle cell injury induced by high glucose via the mitogen-activated protein kinase (MAPK) signaling pathway.MethodsVascular smooth muscle cells (VSMCs) isolated from rat thoracic aortas were divided into control, high glucose (HG), and pre-hatching TMP groups. The effect of different glucose concentr...
3 Citations Source Cite
Published on Nov 1, 2017in Food and Chemical Toxicology 3.77
Jicheng Chen1
Estimated H-index: 1
(Cornell University),
Jingjing Tian2
Estimated H-index: 2
+ 2 AuthorsJianbo Xiao33
Estimated H-index: 33
(UM: University of Macau)
Abstract Tetramethylpyrazine is a bioactive compound found in Chinese black vinegar. This work is to investigate the potential effects of tetramethylpyrazine on intracellular cholesterol modulation in HepG2 cells. The results demonstrated that tetramethylpyrazine can induce intracellular cholesterol efflux. Tetramethylpyrazine may also improve endothelial function through its antioxidant effects by inhibiting reactive oxygen species levels and increasing the antioxidant enzymes superoxide dismut...
5 Citations Source Cite
Published on Sep 1, 2017in Food Chemistry 5.40
Wenjie Jian4
Estimated H-index: 4
(SCAU: South China Agricultural University),
Liying Tu1
Estimated H-index: 1
(JMU: Jimei University)
+ 3 AuthorsYuanming Sun20
Estimated H-index: 20
(SCAU: South China Agricultural University)
Abstract Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100 kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100 kGy. We investigated the protective effect of 100 kGy-KGM against H 2 O 2 induced oxidative damage in LO2 cells. Our results demonstrated that...
8 Citations Source Cite
Published on Apr 1, 2017in Cryobiology 2.14
Joshua Hahn1
Estimated H-index: 1
(University of Alberta Hospital),
Leila Laouar2
Estimated H-index: 2
(University of Alberta Hospital)
+ 2 AuthorsNadr M. Jomha9
Estimated H-index: 9
(University of Alberta Hospital)
Abstract High concentrations of cryoprotective agents are required for cryopreservation techniques such as vitrification. Glycerol is a common cryoprotective agent used in cryopreservation protocols but this agent is toxic at high concentrations. This work is an attempt to mitigate the toxic effects of high concentrations of glycerol on intact chondrocytes in human knee articular cartilage from total knee arthroplasty patients by simultaneous exposure to glycerol and a variety of additive compou...
1 Citations Source Cite
Published on Jul 8, 2016in PLOS ONE 2.78
Shanshan Wang1
Estimated H-index: 1
,
Ting Lei1
Estimated H-index: 1
,
Man Zhang1
Estimated H-index: 1
Chemotherapy is an important strategy for the treatment of bladder cancer. However, the main problem limiting the success of chemotherapy is the development of multidrug resistance (MDR). To improve the management of bladder cancer, it is an urgent matter to search for strategies to reverse MDR. We chose three kinds of herbal medicines including ginsenoside Rh2, (-)-Epigallocatechin gallate (EGCG) and Tetramethylpyrazine (TMP) to detect their effects on bladder cancer. Reversal effects of these ...
5 Citations Source Cite
Published on Feb 1, 2016in Molecular Medicine Reports 1.85
Cheng Yang4
Estimated H-index: 4
(SYSU: Sun Yat-sen University),
Yue Xu7
Estimated H-index: 7
(SYSU: Sun Yat-sen University)
+ 6 AuthorsXiaoling Liang5
Estimated H-index: 5
(SYSU: Sun Yat-sen University)
Abstract Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against apoptosis in endothelial cells. However, the underlying mechanism of its protective effects in endothelial cells remains to be elucidated. Using human umbilical vein endothelial cells (HUVECs), the present study assessed the protective effects of TMP on CoCl2-induced apoptosis. Following pre-incubation with CoCl2 (150 µM/ml) for 4 h, the HUVECs were ...
5 Citations Source Cite
Published on Jan 1, 2016in Oxidative Medicine and Cellular Longevity 4.87
Yingke Zhao2
Estimated H-index: 2
,
Yue Liu1
Estimated H-index: 1
,
Keji Chen3
Estimated H-index: 3
Tetramethylpyrazine, a natural compound from Ligusticum wallichii (Chuan Xiong), has been extensively used in China for cardiovascular and cerebrovascular diseases for about 40 years. Because of its effectiveness in multisystems, especially in cardiovascular, its pharmacological action, clinical application, and the structural modification have attracted broad attention. In this paper its mechanisms of action, the clinical status, and synthetic derivatives will be reviewed briefly.
13 Citations Source Cite
Published on Jan 1, 2016in BioMed Research International 2.20
Ming Guo3
Estimated H-index: 3
,
Yue Liu2
Estimated H-index: 2
,
Da-zhuo Shi11
Estimated H-index: 11
Tetramethylpyrazine (TMP), a pharmacologically active component isolated from the rhizome of the Chinese herb Rhizoma Chuanxiong (Chuanxiong), has been clinically used in China and Southeast Asian countries for the prevention and treatment of cardiovascular diseases (CVDs) for about fifty years. The pharmacological effects of TMP on the cardiovascular system have attracted great interest. Emerging experimental studies and clinical trials have demonstrated that TMP prevents atherosclerosis as wel...
20 Citations Source Cite