scinapse is loading now...

The effect of tetramethylpyrazine on hydrogen peroxide-induced oxidative damage in human umbilical vein endothelial cells.

Published on Oct 1, 2009in Basic & Clinical Pharmacology & Toxicology 2.66
· DOI :10.1111/j.1742-7843.2009.00470.x
Wen-Ming Li9
Estimated H-index: 9
(Chongqing Medical University),
Hongtao Liu18
Estimated H-index: 18
(Chongqing Medical University)
+ 5 AuthorsChao Yu17
Estimated H-index: 17
(Chongqing Medical University)
Abstract
Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H(2)O(2)-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H(2)O(2)-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H(2)O(2) for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H(2)O(2)-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H(2)O(2)-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H(2)O(2)-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H(2)O(2)-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H(2)O(2)-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
  • References (37)
  • Citations (35)
Cite
References37
Dang Sc1
Estimated H-index: 1
,
Jie Zhang10
Estimated H-index: 10
+ 2 AuthorsFan X1
Estimated H-index: 1
15 Citations
Published on Jul 1, 1993in The EMBO Journal 10.56
R de Martin5
Estimated H-index: 5
,
B. Vanhove2
Estimated H-index: 2
+ 4 AuthorsFritzH. Bach82
Estimated H-index: 82
Abstract The transient expression of many different genes is mediated by the inducible transcription factor p50-p65 NF kappa B, which in turn is regulated by complex formation with its inhibitor I kappa B alpha. We describe here that in porcine aortic endothelial cells, either IL-1 alpha, TNF alpha or LPS upregulates an inhibitor of NF kappa B which we refer to as ECI-6. ECI-6 is by structural and functional criteria an I kappa B alpha protein, the porcine homologue of MAD-3, pp40 and RL/IF-1. W...
247 Citations Source Cite
Published on Jan 1, 2003in Biological & Pharmaceutical Bulletin 1.69
An-Shu Chen4
Estimated H-index: 4
(Meijo University),
Tadao Taguchi9
Estimated H-index: 9
(Meijo University)
+ 3 AuthorsIchitomo Miwa23
Estimated H-index: 23
(Meijo University)
aminoguanidine, pyridoxamine, and Trolox (their IC50 values518, 80, 85, 10, and 95 m M, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC50 values of the 2 oligomers were 30 and 55 m M, respectively. Such a scavenging activity of these 2 chit...
48 Citations Source Cite
Published on Oct 1, 2007in Anesthesia & Analgesia 3.46
Baohua Wang5
Estimated H-index: 5
,
Tao Luo2
Estimated H-index: 2
+ 1 AuthorsDavid M. Ansley18
Estimated H-index: 18
BACKGROUND: Vascular endothelial cells play an important role in maintaining cardiovascular homeostasis. Oxidative stress is a critical pathogenic factor in endothelial cell damage and the development of cardiovascular diseases. In this study we evaluated the effects of propofol on oxidative stress-induced endothelial cell insults and the role of serine-threonine kinase Akt modulation of endothelial nitric oxide synthase (eNOS) as a mechanism of protection. METHODS: Human umbilical vein endothel...
48 Citations Source Cite
Published on Aug 1, 2007in Phytomedicine 3.61
Chang-suo Liu2
Estimated H-index: 2
(Peking Union Medical College),
Nai-Hong Chen25
Estimated H-index: 25
(Peking Union Medical College),
Jun-tian Zhang7
Estimated H-index: 7
(Peking Union Medical College)
Abstract A number of studies indicate that reactive oxygen species (ROS) are involved in neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The neuroprotective effects of salvianolic acid B (SalB) from Radix Salviae miltiorrhizae (RSM) against hydrogen peroxide (H 2 O 2 )-induced rat pheochromocytoma line PC12 injury were evaluated in the present study. Vitamin E, a potent antioxidant, was employed as a positive control agent. Following exposure of cells t...
71 Citations Source Cite
Published on May 1, 2007in Life Sciences 3.23
Eresha Mendis19
Estimated H-index: 19
(Pukyong National University),
Moon-Moo Kim28
Estimated H-index: 28
(Pukyong National University)
+ 1 AuthorsSe-Kwon Kim81
Estimated H-index: 81
(Pukyong National University)
Abstract Despite extensive study on biological activities of chitosan and chitooligosaccharides (COS), there is no experimental evidence available as to COS mediated inhibition of free radical damage in cellular oxidizing systems. In this study, radical scavenging efficacies of different molecular weight bearing COS were assessed and their intracellular radical scavenging effects were tested employing B16F1, murine melanoma cell line. The results exhibited appreciable suppression in occurrence o...
81 Citations Source Cite
Published on Oct 1, 2005in Molecular and Cellular Biochemistry 2.56
Ramasamy P. Pandian13
Estimated H-index: 13
(Ohio State University),
Vijay Kumar Kutala23
Estimated H-index: 23
(Ohio State University)
+ 2 AuthorsPeriannan Kuppusamy44
Estimated H-index: 44
(Ohio State University)
Oxygen consumption rate (OCR) and generation of superoxide and nitric oxide (NO) in mouse aortic endothelial cells (MAECs) treated with lipopolysaccharide (LPS) were studied. The OCR was determined in cell suspensions at 37 °C by electron paramagnetic resonance (EPR) spectroscopy. LPS significantly altered the OCR in a dose and time-dependent fashion. The OCR was significantly elevated immediately following the treatment of MAECs with LPS (5 and 10 μg/ml) and NADPH (100 μM) whereas the same was ...
22 Citations Source Cite
Published on Sep 1, 2004in Transplantation Proceedings 0.81
L. Feng7
Estimated H-index: 7
(Sichuan University),
Y. Xiong1
Estimated H-index: 1
(Sichuan University)
+ 3 AuthorsLi Y33
Estimated H-index: 33
(Sichuan University)
Abstract Introduction Ischemia-reperfusion (I/R) injury is unavoidable in cadaveric renal transplantation. It contributes to acute rejection and chronic allograft dysfunction. Studies have shown that Ligustrazine, a purified and chemically identified component of a Chinese herbal remedy, is a potent blocker of vasoconstriction and has strong effects to scavenge oxygen free radicals. Since warm I/R is potentially more damaging than cold storage, we investigated the possible protective effect of L...
57 Citations Source Cite
Published on Mar 1, 2002in Neuroreport 1.27
Yang-Hsin Shih25
Estimated H-index: 25
,
Se-Ling Wu1
Estimated H-index: 1
+ 3 AuthorsYu-Show Fu13
Estimated H-index: 13
Tetramethylpyrazine (TMP) is the major component extracted from the Chinese herb, Chuanxiong. This study focuses on the protective effect of tetramethylpyrazine in kainate-induced excitotoxicity in rat hippocampus. Primary neuronal cultures raised from cells isolated from the hippocampi of 7-day old rats were treated with kainate (75-450 pM) for 12, 24, and 48 h. Our results revealed that kainate induced neuronal damage in a dose- and time-dependent manner, reaching maximal damage at 150 μM and ...
56 Citations Source Cite
Published on Jan 1, 2007in Cerebrovascular Diseases 2.93
Guillermo Zalba34
Estimated H-index: 34
,
Ana Fortuño29
Estimated H-index: 29
+ 3 AuthorsJavier Díez43
Estimated H-index: 43
Endothelial dysfunction is a marker of atherosclerosis and contributes to the atherogenic process and the development of atherothrombotic complications. Oxidative stress has been implicated in the dev
43 Citations Source Cite
Cited By35
Published on Oct 1, 2012in Biomedical Chromatography 1.69
Jinzhao Han3
Estimated H-index: 3
(Second Military Medical University),
Jun Sun6
Estimated H-index: 6
(Second Military Medical University)
+ 3 AuthorsFang Chen1
Estimated H-index: 1
(Second Military Medical University)
Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent...
12 Citations Source Cite
Published on May 1, 2012in Chemical Biology & Drug Design 2.33
Lijuan Deng1
Estimated H-index: 1
(Shandong University),
Xiu-Li Guo14
Estimated H-index: 14
(Shandong University)
+ 5 AuthorsXinyong Liu28
Estimated H-index: 28
(Shandong University)
A series of novel stilbene derivatives containing ligustrazinyl moiety was designed, synthesized, and assayed for their protective effects on damaged endothelial cells. The results showed that most ligustrazinyl stilbene derivatives exhibited high protective effects on the human umbilical vascular endothelial cells (HUVECs) damaged by hydrogen peroxide in comparison with Ligustrazine. The stilbene derivatives A6, A9, A11, A21, A24, A25, and A27 exhibited high potency with low EC50 values ranged ...
13 Citations Source Cite
Published on Jan 1, 2012in Advances in Botanical Research 1.39
Cindy Lh Yang2
Estimated H-index: 2
(Li Ka Shing Faculty of Medicine, University of Hong Kong),
Terry C.T. Or4
Estimated H-index: 4
(University of Hong Kong)
+ 1 AuthorsAllan S. Y. Lau25
Estimated H-index: 25
(University of Hong Kong)
Abstract Stroke is not only one of the leading causes of mortality but also associated with long-term morbidity worldwide. Due to the adverse side effects and short therapeutic window of current drugs, traditional Chinese medicine (TCM) is getting more popular to treat stroke-related diseases. In this review, we will summarize recent publications of pharmacological effects of several medicinal decoctions containing Ligusticum chuanxiong (LCX) and its active ingredients on cerebrovascular disease...
2 Citations Source Cite
Published on Jan 1, 2016in Journal of Cellular Biochemistry 2.96
Liang Wang2
Estimated H-index: 2
(Hong Kong Polytechnic University),
Xiaojing Zhang4
Estimated H-index: 4
(Jinan University)
+ 11 AuthorsQing-Wen Zhang19
Estimated H-index: 19
(University of Macau)
Doxorubicin (Dox) is an anthracycline antibiotic widely used in clinics as an anticancer agent. However, the use of Dox is limited by its cardiotoxicity. We have previously shown that a Danshensu (DSS) derivative, ADTM, displayed strong cardioprotective effects. With improved chemical stability and activity, a novel DSS derivative, D006, based on the structure of ADTM, was synthesized. In the present study, the protective effects of D006, indexed by attenuation of the cardiotoxicity induced by D...
12 Citations Source Cite
Published on May 1, 2015in Oncology Reports 2.98
Xiao-Jing Wang3
Estimated H-index: 3
,
You-Hua Xu3
Estimated H-index: 3
+ 2 AuthorsPing Zhang3
Estimated H-index: 3
5 Citations Source Cite
Published on Jan 1, 2012in European Journal of Pharmacology 3.04
Hong-Jin Wu1
Estimated H-index: 1
,
Jing Hao1
Estimated H-index: 1
+ 2 AuthorsXianbing Chen2
Estimated H-index: 2
Abstract To investigate the effects of Ligustrazine, a compound derived from chuanxiong, on tumor necrosis factor-α (TNF-α) stimulated endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α in vitro. Nitric oxide (NO) was measured as a standard of endothelial dysfunction. Two important indicators of autoimmunity, intracellular adhesion molecular-1 (ICAM-1) and heat shock protein 60 (HSP60), were selected to evaluate the influence of Ligustrazine on HUVECs. ...
21 Citations Source Cite
Published on Sep 1, 2013in European Journal of Pharmacology 3.04
Erik J.B. Ruijters5
Estimated H-index: 5
(Maastricht University),
Antje R. Weseler16
Estimated H-index: 16
(Maastricht University)
+ 2 AuthorsAalt Bast60
Estimated H-index: 60
(Maastricht University)
Abstract Accumulating evidence suggests that foods rich in flavanols decrease the risk of developing cardiovascular diseases. Attenuation of oxidative stress was suggested to contribute to the cardiovascular benefit of flavanols. Up to now it was unclear whether flavanol metabolites can also protect cells from oxidative stress. The aim of the present study was to determine the potential contribution of several glucuronidated, methylated and sulfated metabolites of (-)-epicatechin (EC) and (+)-ca...
48 Citations Source Cite
Published on Aug 1, 2012in Glycoconjugate Journal 2.55
Peng Wei6
Estimated H-index: 6
(Chinese Academy of Sciences),
Pan Ma5
Estimated H-index: 5
(Chinese Academy of Sciences)
+ 5 AuthorsChao Yu17
Estimated H-index: 17
(Chongqing Medical University)
Chitosan oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. In the present study, we examined the effect of COS on nitric oxide (NO) production in LPS induced N9 microglial cells. Pretreatment with COS (50 ~ 200 μg/ml) could markedly inhibit NO production by suppressing inducible nitric oxide synthase (iNOS) expression in activated microglial cells. Signal transduction studies showed that COS remarkably inh...
23 Citations Source Cite
Published on Jun 1, 2014in Toxicology in Vitro 3.10
Kristien J.A. Lemmens5
Estimated H-index: 5
(Maastricht University),
Bregje van de Wier3
Estimated H-index: 3
(Maastricht University)
+ 5 AuthorsGuido R.M.M. Haenen55
Estimated H-index: 55
(Maastricht University)
Abstract The flavonoid 7-mono-O-(β-hydroxyethyl)-rutoside (monoHER) is an effective protector against doxorubicin induced toxicity which has been related to the antioxidant activity of monoHER. The present study examines the potential relevance of the direct scavenging activity of the flavonoid. The potency of the direct antioxidant effect was confirmed by its instantaneous protection against intracellular oxidative stress in human umbilical vein endothelial cells at therapeutically achievable c...
14 Citations Source Cite
Guilan Sun5
Estimated H-index: 5
(Shanghai University),
Xiao Lin13
Estimated H-index: 13
(Shanghai University)
+ 4 AuthorsYi Feng17
Estimated H-index: 17
(Shanghai University)
A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous determination of gastrodin (1), ferulic acid (2), and senkyunolide I (3) in rat plasma after oral administration of a famous traditional Chinese medicine, that is, Da ChuanXiong Fang. The analysis was performed on a reversed-phase C18 column with a gradient eluent composed of acetonitrile and 1% acetic acid. The flow rate was kept at 1 mL/min and the detection wavelengths were set at 270, 322...
Source Cite