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The Effect of Tetramethylpyrazine on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells
Abstract
Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H(2)O(2)-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H(2)O(2)-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H(2)O(2) for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H(2)O(2)-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H(2)O(2)-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H(2)O(2)-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H(2)O(2)-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H(2)O(2)-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
  • References (37)
  • Cited By (35)
1993 in The EMBO Journal [IF: 9.79]
R de Martin5
Estimated H-index: 5
,
B. Vanhove4
Estimated H-index: 4
,
Qi Cheng2
Estimated H-index: 2
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Abstract The transient expression of many different genes is mediated by the inducible transcription factor p50-p65 NF kappa B, which in turn is regulated by complex formation with its inhibitor I kappa B alpha. We describe here that in porcine aortic endothelial cells, either IL-1 alpha, TNF alpha or LPS upregulates an inhibitor of NF kappa B which we refer to as ECI-6. ECI-6 is by structural and functional criteria an I kappa B alpha protein, the porcine homologue of MAD-3, pp40 and RL/IF-1. W...
Cited 243
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An-Shu Chen4
Estimated H-index: 4
(Meijo University),
Tadao Taguchi9
Estimated H-index: 9
(Meijo University),
Kazuo Sakai7
Estimated H-index: 7
... more
aminoguanidine, pyridoxamine, and Trolox (their IC50 values518, 80, 85, 10, and 95 m M, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC50 values of the 2 oligomers were 30 and 55 m M, respectively. Such a scavenging activity of these 2 chit...
Ref 28Cited 48
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2007 in Anesthesia & Analgesia [IF: 4.01]
Baohua Wang6
Estimated H-index: 6
,
Tao Luo3
Estimated H-index: 3
,
David D. Y. Chen31
Estimated H-index: 31
... more
BACKGROUND: Vascular endothelial cells play an important role in maintaining cardiovascular homeostasis. Oxidative stress is a critical pathogenic factor in endothelial cell damage and the development of cardiovascular diseases. In this study we evaluated the effects of propofol on oxidative stress-induced endothelial cell insults and the role of serine-threonine kinase Akt modulation of endothelial nitric oxide synthase (eNOS) as a mechanism of protection. METHODS: Human umbilical vein endothel...
Ref 32Cited 46
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Chang-suo Liu2
Estimated H-index: 2
(Peking Union Medical College),
Nai-hong Chen4
Estimated H-index: 4
(Peking Union Medical College),
Jun-Tian Zhang4
Estimated H-index: 4
(Peking Union Medical College)
Abstract A number of studies indicate that reactive oxygen species (ROS) are involved in neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The neuroprotective effects of salvianolic acid B (SalB) from Radix Salviae miltiorrhizae (RSM) against hydrogen peroxide (H 2 O 2 )-induced rat pheochromocytoma line PC12 injury were evaluated in the present study. Vitamin E, a potent antioxidant, was employed as a positive control agent. Following exposure of cells t...
Ref 16Cited 71
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2007 in Life Sciences [IF: 2.94]
Eresha Mendis19
Estimated H-index: 19
(Pukyong National University),
Moon-Moo Kim28
Estimated H-index: 28
(Pukyong National University),
Niranjan Rajapakse19
Estimated H-index: 19
(Pukyong National University)
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Abstract Despite extensive study on biological activities of chitosan and chitooligosaccharides (COS), there is no experimental evidence available as to COS mediated inhibition of free radical damage in cellular oxidizing systems. In this study, radical scavenging efficacies of different molecular weight bearing COS were assessed and their intracellular radical scavenging effects were tested employing B16F1, murine melanoma cell line. The results exhibited appreciable suppression in occurrence o...
Ref 28Cited 80
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Ramasamy P. Pandian13
Estimated H-index: 13
(Ohio State University),
Vijay Kumar Kutala23
Estimated H-index: 23
(Ohio State University),
Alex Liaugminas1
Estimated H-index: 1
(Ohio State University)
... more
Oxygen consumption rate (OCR) and generation of superoxide and nitric oxide (NO) in mouse aortic endothelial cells (MAECs) treated with lipopolysaccharide (LPS) were studied. The OCR was determined in cell suspensions at 37 °C by electron paramagnetic resonance (EPR) spectroscopy. LPS significantly altered the OCR in a dose and time-dependent fashion. The OCR was significantly elevated immediately following the treatment of MAECs with LPS (5 and 10 μg/ml) and NADPH (100 μM) whereas the same was ...
Ref 43Cited 22
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2004 in Transplantation Proceedings [IF: 0.91]
Li Feng12
Estimated H-index: 12
(Sichuan University),
Y. Xiong1
Estimated H-index: 1
(Sichuan University),
F. Cheng6
Estimated H-index: 6
(Sichuan University)
... more
Abstract Introduction Ischemia-reperfusion (I/R) injury is unavoidable in cadaveric renal transplantation. It contributes to acute rejection and chronic allograft dysfunction. Studies have shown that Ligustrazine, a purified and chemically identified component of a Chinese herbal remedy, is a potent blocker of vasoconstriction and has strong effects to scavenge oxygen free radicals. Since warm I/R is potentially more damaging than cold storage, we investigated the possible protective effect of L...
Ref 15Cited 56
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2002 in Neuroreport
Yang-Hsin Shih24
Estimated H-index: 24
,
Se-Ling Wu1
Estimated H-index: 1
,
Wen-Fei Chiou1
Estimated H-index: 1
... more
Tetramethylpyrazine (TMP) is the major component extracted from the Chinese herb, Chuanxiong. This study focuses on the protective effect of tetramethylpyrazine in kainate-induced excitotoxicity in rat hippocampus. Primary neuronal cultures raised from cells isolated from the hippocampi of 7-day old rats were treated with kainate (75–450 μM) for 12, 24, and 48 h. Our results revealed that kainate induced neuronal damage in a dose- and time-dependent manner, reaching maximal damage at 150 μM and ...
Ref 21Cited 55
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Guillermo Zalba34
Estimated H-index: 34
,
Ana Fortuño29
Estimated H-index: 29
,
Gorka San José22
Estimated H-index: 22
... more
Endothelial dysfunction is a marker of atherosclerosis and contributes to the atherogenic process and the development of atherothrombotic complications. Oxidative stress has been implicated in the dev
Ref 45Cited 43
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  • Cited By (35)
2012 in Biomedical Chromatography [IF: 1.61]
Jinzhao Han3
Estimated H-index: 3
(Second Military Medical University),
Jun Sun6
Estimated H-index: 6
(Second Military Medical University),
Quangang Zhu13
Estimated H-index: 13
(Second Military Medical University)
... more
Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent...
Ref 20Cited 12
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Lijuan Deng1
Estimated H-index: 1
(Shandong University),
Xiu-Li Guo14
Estimated H-index: 14
(Shandong University),
Li Zhai2
Estimated H-index: 2
(Shandong University)
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A series of novel stilbene derivatives containing ligustrazinyl moiety was designed, synthesized, and assayed for their protective effects on damaged endothelial cells. The results showed that most ligustrazinyl stilbene derivatives exhibited high protective effects on the human umbilical vascular endothelial cells (HUVECs) damaged by hydrogen peroxide in comparison with Ligustrazine. The stilbene derivatives A6, A9, A11, A21, A24, A25, and A27 exhibited high potency with low EC50 values ranged ...
Ref 22Cited 13
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Cindy Lh Yang2
Estimated H-index: 2
(Li Ka Shing Faculty of Medicine, University of Hong Kong),
Terry C.T. Or4
Estimated H-index: 4
(University of Hong Kong),
Jonathan S.H. Lau2
Estimated H-index: 2
(University of Hong Kong)
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Abstract Stroke is not only one of the leading causes of mortality but also associated with long-term morbidity worldwide. Due to the adverse side effects and short therapeutic window of current drugs, traditional Chinese medicine (TCM) is getting more popular to treat stroke-related diseases. In this review, we will summarize recent publications of pharmacological effects of several medicinal decoctions containing Ligusticum chuanxiong (LCX) and its active ingredients on cerebrovascular disease...
Ref 91Cited 2
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Liang Wang2
Estimated H-index: 2
(Hong Kong Polytechnic University),
Xiaojing Zhang4
Estimated H-index: 4
(Jinan University),
Judy Yuet-Wa Chan22
Estimated H-index: 22
(University of Macau)
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Doxorubicin (Dox) is an anthracycline antibiotic widely used in clinics as an anticancer agent. However, the use of Dox is limited by its cardiotoxicity. We have previously shown that a Danshensu (DSS) derivative, ADTM, displayed strong cardioprotective effects. With improved chemical stability and activity, a novel DSS derivative, D006, based on the structure of ADTM, was synthesized. In the present study, the protective effects of D006, indexed by attenuation of the cardiotoxicity induced by D...
Ref 46Cited 12
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2015 in Oncology Reports [IF: 2.66]
Xiao-Jing Wang3
Estimated H-index: 3
,
You-Hua Xu3
Estimated H-index: 3
,
Gui-Cun Yang3
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Hong-Jin Wu1
Estimated H-index: 1
,
Jing Hao1
Estimated H-index: 1
,
Shengqi Wang4
Estimated H-index: 4
... more
Abstract To investigate the effects of Ligustrazine, a compound derived from chuanxiong, on tumor necrosis factor-α (TNF-α) stimulated endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α in vitro. Nitric oxide (NO) was measured as a standard of endothelial dysfunction. Two important indicators of autoimmunity, intracellular adhesion molecular-1 (ICAM-1) and heat shock protein 60 (HSP60), were selected to evaluate the influence of Ligustrazine on HUVECs. ...
Ref 32Cited 19
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Erik J.B. Ruijters5
Estimated H-index: 5
(Maastricht University),
Antje R. Weseler10
Estimated H-index: 10
(Maastricht University),
Cécile H. Kicken5
Estimated H-index: 5
(Maastricht University)
... more
Abstract Accumulating evidence suggests that foods rich in flavanols decrease the risk of developing cardiovascular diseases. Attenuation of oxidative stress was suggested to contribute to the cardiovascular benefit of flavanols. Up to now it was unclear whether flavanol metabolites can also protect cells from oxidative stress. The aim of the present study was to determine the potential contribution of several glucuronidated, methylated and sulfated metabolites of (-)-epicatechin (EC) and (+)-ca...
Ref 33Cited 47
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Peng Wei6
Estimated H-index: 6
(Chinese Academy of Sciences),
Pan Ma4
Estimated H-index: 4
(Chinese Academy of Sciences),
Qingsong Xu5
Estimated H-index: 5
(Chinese Academy of Sciences)
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Chitosan oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. In the present study, we examined the effect of COS on nitric oxide (NO) production in LPS induced N9 microglial cells. Pretreatment with COS (50 ~ 200 μg/ml) could markedly inhibit NO production by suppressing inducible nitric oxide synthase (iNOS) expression in activated microglial cells. Signal transduction studies showed that COS remarkably inh...
Ref 43Cited 21
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2014 in Toxicology in Vitro [IF: 2.87]
Kristien J.A. Lemmens5
Estimated H-index: 5
(Maastricht University),
Bregje van de Wier2
Estimated H-index: 2
(Maastricht University),
Nathalie Vaes2
Estimated H-index: 2
(Maastricht University)
... more
Abstract The flavonoid 7-mono-O-(β-hydroxyethyl)-rutoside (monoHER) is an effective protector against doxorubicin induced toxicity which has been related to the antioxidant activity of monoHER. The present study examines the potential relevance of the direct scavenging activity of the flavonoid. The potency of the direct antioxidant effect was confirmed by its instantaneous protection against intracellular oxidative stress in human umbilical vein endothelial cells at therapeutically achievable c...
Ref 33Cited 14
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