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A proteomic approach to characterizing ciglitazone-induced cancer cell differentiation in Hep-G2 cell line

Published on Apr 1, 2009in Biochimica et Biophysica Acta3.79
· DOI :10.1016/j.bbapap.2009.01.006
Patrizia Bottoni21
Estimated H-index: 21
(UCSC: Catholic University of the Sacred Heart),
Bruno Giardina46
Estimated H-index: 46
(UCSC: Catholic University of the Sacred Heart)
+ 2 AuthorsRoberto Scatena23
Estimated H-index: 23
(UCSC: Catholic University of the Sacred Heart)
Abstract
Drug induced cell differentiation represents a promising experimental model for proteomic analysis of cancer cells. In fact, by modulating and monitoring neoplastic cell differentiation it could be possible to identify cytodifferentiation related protein expression changes that can be subsequently utilized in vivo as potential cancer biomarkers. One main advantage of this approach is the significant reduction of biological variability normally observed in clinical biomarker research, with important implications also in prognosis and therapy. At this regard, a new class of differentiating agents is emerging, the so called PPAR-ligands, which however are characterized by a debated mechanism of action that has not been yet studied through a proteomic approach. To this aim, we investigated ciglitazone-induced differentiation of a human hepatocarcinoma HepG2 cell line, by monitoring biochemical and cellular parameters of cytodifferentiation and modifications of cellular protein profiles through 2-DE and MALDI-TOF analysis. Independent of the hypothesized mechanism of action of this intriguing PPARγ agonist, results indicated that ciglitazone is a strong differentiating agent for the HepG2 cell line and that this process is associated with modifications of protein expression related to cell antioxidant systems, the cell cycle apparatus, signal transduction pathways, cellular stress and invasiveness. At last, considering these and other published data, a proteomic profile related to the cancer aggressiveness is beginning to emerge.
  • References (75)
  • Citations (12)
References75
Newest
#1Roberto Scatena (UCSC: Catholic University of the Sacred Heart)H-Index: 23
#2Patrizia Bottoni (UCSC: Catholic University of the Sacred Heart)H-Index: 21
Last.Bruno Giardina (UCSC: Catholic University of the Sacred Heart)H-Index: 46
view all 3 authors...
#1Huirong Yang (Fudan University)H-Index: 4
#2Jian Chen (NU: Northwestern University)H-Index: 1
Last.Long Yu (Fudan University)H-Index: 33
view all 6 authors...
#1Yangchao Chen (CUHK: The Chinese University of Hong Kong)H-Index: 25
#2Marie Chia-mi Lin (HKU: University of Hong Kong)H-Index: 14
Last.David T. Yew (CUHK: The Chinese University of Hong Kong)H-Index: 35
view all 12 authors...
#1Carola A. Neumann (MUSC: Medical University of South Carolina)H-Index: 9
#2Quan Fang (MUSC: Medical University of South Carolina)H-Index: 1
#1Roberto Scatena (UCSC: Catholic University of the Sacred Heart)H-Index: 23
#2Patrizia Bottoni (UCSC: Catholic University of the Sacred Heart)H-Index: 21
Last.Bruno Giardina (UCSC: Catholic University of the Sacred Heart)H-Index: 46
view all 7 authors...
#1Kensuke Kojima (University of Texas MD Anderson Cancer Center)H-Index: 23
#2Marina Konopleva (VCU: Virginia Commonwealth University)H-Index: 1
Last.Michael Andreeff (VCU: Virginia Commonwealth University)H-Index: 2
view all 5 authors...
#1Jeremy Ts Dabbeekeh (Roswell Park Cancer Institute)H-Index: 2
#2Silviu L. Faitar (Roswell Park Cancer Institute)H-Index: 7
Last.John Kenneth Cowell (Roswell Park Cancer Institute)H-Index: 45
view all 4 authors...
Cited By12
Newest
#1Patrizia Bottoni (CUA: The Catholic University of America)H-Index: 21
#2Roberto Scatena (CUA: The Catholic University of America)H-Index: 23
#1Patrizia Bottoni (CUA: The Catholic University of America)H-Index: 21
#2Maria Antonietta Isgrò (UCSC: Catholic University of the Sacred Heart)H-Index: 8
Last.Roberto Scatena (CUA: The Catholic University of America)H-Index: 23
view all 3 authors...
#1Patrizia Bottoni (CUA: The Catholic University of America)H-Index: 21
#2Bruno Giardina (CUA: The Catholic University of America)H-Index: 46
Last.Roberto Scatena (CUA: The Catholic University of America)H-Index: 23
view all 5 authors...
#1Roberto Scatena (CUA: The Catholic University of America)H-Index: 23
#2Patrizia Bottoni (CUA: The Catholic University of America)H-Index: 21
Last.Bruno Giardina (CUA: The Catholic University of America)H-Index: 46
view all 4 authors...
#1Yaşam Barlak (Gümüşhane University)H-Index: 1
#2Orhan Deger (Karadeniz Technical University)H-Index: 25
Last.Fulya Balaban Yucesan (Karadeniz Technical University)H-Index: 8
view all 6 authors...
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