Determination of Bradykinin B2 Receptor in Vivo Phosphorylation Sites and Their Role in Receptor Function

Reversible phosphorylation plays important roles in G protein-coupled receptor signaling, desensitization, and endocytosis, yet the precise location and role of in vivophosphorylation sites is unknown for most receptors. Using metabolic32P labeling and phosphopeptide sequencing we provide a complete phosphorylation map of the human bradykinin B2receptor in its native cellular environment. We identified three serine residues, Ser339, Ser346, and...