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Evidence of persistent measles virus infection in Crohn's disease.

Published on Apr 1, 1993in Journal of Medical Virology2.05
· DOI :10.1002/jmv.1890390415
Aj Wakefield25
Estimated H-index: 25
(Royal Free Hospital),
R.M Pittilo2
Estimated H-index: 2
(KUL: Kingston University)
+ 4 AuthorsRoy E. Pounder49
Estimated H-index: 49
(Royal Free Hospital)
Cite
Abstract
Transmission electron microscopy was used to examine the microvasculature of perfusion-fixed tissues from Crohn's disease and control patients. Paramyxovirus-like particles, and inclusions consisting of condensations of nucleocapsid, in giant cells and endothelium at foci of vascular injury were identified in all 9 Crohn's disease patients. Tissues from patients with Crohn's disease were also examined by either in situ hybridisation (n = 10) or immunohistochemistry (n = 15), and compared to inflammatory and noninflammatory controls (n = 22). Hybridisation for measles virus N-protein genomic RNA was positive in all cases of Crohn's disease localising to foci of granulomatous vasculitis and lymphoid follicles. Positive immunohistochemical staining for measles virus nucleocapsid protein was positive in 13 of 15 patients with Crohn's disease, localising to foci of granulomatous inflammation. Hybridisation for measles virus RNA was positive in a minority of control intestinal tissues; viral inclusions were not seen ultrastructurally. Immunostaining was negative in control cases of intestinal tuberculosis. These observations suggest that measles virus is capable of causing persistent infection of the intestine and that Crohn's disease may be caused by a granulomatous vasculitis in response to this virus.
  • References (19)
  • Citations (209)
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References19
Newest
Published on Nov 1, 1992in Journal of Medical Virology2.05
Andrew J. Wakefield28
Estimated H-index: 28
(Royal Free Hospital),
J. D. Fox1
Estimated H-index: 1
+ 7 AuthorsRoy E. Pounder49
Estimated H-index: 49
The prevalence of herpesvirus DNA was examined in inflammatory bowel disease tissue. DNA was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with Crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). The nested polymerase chain reaction was used to detect viral DNA using primer pairs specific for either cytomegalovirus (CMV), herpes simplex virus 1 (HSV1), human herpesvirus 6 (H...
Published on Sep 19, 1992in BMJ27.60
Michael B. Prentice20
Estimated H-index: 20
Published on May 1, 1992in Gastroenterology19.23
M. Hudson19
Estimated H-index: 19
(Royal Free Hospital),
C Piasecki10
Estimated H-index: 10
(Royal Free Hospital)
+ 6 AuthorsRoy E. Pounder49
Estimated H-index: 49
(Royal Free Hospital)
Abstract Based on the demonstration of mural granulomatous vasculitis in Crohn's disease, it was hypothesized that this vasculitis may account for the discontinuous pattern of lesions in this condition. Accordingly, the present study investigated the histological changes produced by interruption of the submucosal and mucosal microcirculation in the ferret midgut. Two techniques were used. First, up to 30 adjacent vasa recta were ligated using microsurgical techniques; this produced no evidence o...
Published on Jan 1, 1992in Ultrastructural Pathology1.20
Samuel P. Hammar32
Estimated H-index: 32
,
Jenyung Luu3
Estimated H-index: 3
+ 4 AuthorsMichael A. Bean4
Estimated H-index: 4
Tubuloreticular structures were induced in human umbilical vein endothelial cells cultured in media containing recombinant interferon alfa and beta but not in media containing recombinant interferon gamma or other agents that induce interferon, such as 5-bromodeoxyuridine or polyinosinic-polycytidylic acid. Recombinant interferon beta induced tubuloreticular structures in endothelial cells at a lower concentration and in a greater percentage of cell sections than recombinant interferon alfa. Thi...
Published on May 1, 1991in Gastroenterology19.23
Andrew J. Wakefield28
Estimated H-index: 28
(UCL: University College London),
Elizabeth A. Sankey1
Estimated H-index: 1
(UCL: University College London)
+ 8 AuthorsAdam A.M. Lewis2
Estimated H-index: 2
(UCL: University College London)
Abstract This study investigated a possible vascular origin for granulomas in Crohn's disease. Twenty-four consecutive resected specimens of small and large intestinal Crohn's disease were preserved by arterial perfusion-fixation with 10% formol saline, at mean arterial pressure (100 mm Hg). Fifteen specimens contained granulomas on routine examination of HE they were found most frequently in the submucosa (42%). The techniques used in this study enhanced the recognition of granulomatous vasculi...
Published on Dec 1, 1990in American Journal of Epidemiology4.47
Anders Ekbom98
Estimated H-index: 98
,
Hans-Olov Adami129
Estimated H-index: 129
+ 2 AuthorsMatthew M. Zack49
Estimated H-index: 49
Published on Aug 1, 1990in Journal of Medical Virology2.05
Sadao Suga36
Estimated H-index: 36
(Fujita Health University),
Tetsushi Yoshikawa37
Estimated H-index: 37
(Fujita Health University)
+ 2 AuthorsShigeru Yoshida4
Estimated H-index: 4
(Osaka University)
Two infants (4 and 5 months of age) with a febrile episode for 3 and 5 days, respectively, developed skin rashes after the fever subsided and were diagnosed as exanthem subitum. The rash continued for 5 days followed by mild-to-moderate pigmentation. Human herpesvirus-6 and measles virus, which were confirmed by a specific immunofluorescence assay and by electron microscopy, were isolated simultaneously from blood in the acute stage of the disease but not from the convalescent stage. The titer o...
Published on Apr 1, 1990in Journal of Clinical Pathology2.35
Stephen McQuaid38
Estimated H-index: 38
,
Sylvie Isserte3
Estimated H-index: 3
+ 3 AuthorsS. L. Cosby19
Estimated H-index: 19
Optimised immunocytochemical (ICC) and in situ hybridisation (ISH) protocols for long term, formalin fixed, central nervous system tissue infected with measles virus were developed. The effectiveness of 10 proteases for the enzymatic unmasking of formalin fixed antigen and nucleic acid was investigated. Protease VIII gave maximal signal generation with optimal tissue preservation and no background staining for both techniques. The use of a microwave oven as an additional pre-hybridisation step f...
Published on Mar 1, 1990in Gastroenterology19.23
H.O. Kangro2
Estimated H-index: 2
(St Bartholomew's Hospital),
S.K.F. Chong1
Estimated H-index: 1
(St Bartholomew's Hospital)
+ 2 AuthorsJ.A. Walker-Smith1
Estimated H-index: 1
(St Bartholomew's Hospital)
Abstract Seventy-two children with chronic inflammatory bowel disease were investigated for infections with various viruses and Mycoplasma pneumoniae, Chlamydia psittaci, and Coxiella burnetii to determine whether these pathogens are associated with acute onset exacerbations. Altogether 54 infections were identified serologically, of which 23 (42.6%) were associated with exacerbations. This corresponded to 24.2% of the recorded exacerbations during the study period. The respiratory pathogens acc...
Published on Nov 1, 1989in The Lancet59.10
Aj Wakefield25
Estimated H-index: 25
(Royal Free Hospital),
Amar P. Dhillon59
Estimated H-index: 59
(Royal Free Hospital)
+ 4 AuthorsRoy E. Pounder49
Estimated H-index: 49
(Royal Free Hospital)
Abstract In a prospective study, specimens of resected small and large intestine from fifteen patients with Crohn's disease were prepared by heparin-saline vascular perfusion, followed by either resin casting of the mesenteric vascular supply and tissue maceration or glutaraldehyde perfusion-fixation, resin casting, and tissue clearance. The specimens were examined by macrophotography, histopathology, and either scanning or transmission electronmicroscopy. A pathogenetic sequence of events in Cr...
Cited By209
Newest
Published on Dec 1, 2018in Human Pathology2.74
Maryam Kherad Pezhouh1
Estimated H-index: 1
(NU: Northwestern University),
James Adam Miller3
Estimated H-index: 3
(Johns Hopkins University)
+ 7 AuthorsLysandra Voltaggio10
Estimated H-index: 10
(Johns Hopkins University)
Summary A potential role for viral infections has been implicated in inflammatory bowel disease (IBD) unresponsive to medical treatment. It is well known that Epstein-Barr virus (EBV) infection can elicit a brisk mononuclear response in the gastrointestinal tract. The aim of this study was to further evaluate the role of EBV in patients with refractory IBD and compare them with nonrefractory IBD cases. Surgically resected colonic specimens from 67 patients with refractory IBD (62 with ulcerative...
Published on Sep 20, 2018in PLOS Pathogens6.46
Serre-Yu Wong2
Estimated H-index: 2
(NYU: New York University),
Ken Cadwell29
Estimated H-index: 29
(NYU: New York University)
Published on Jan 1, 2018
Kevin Mullane12
Estimated H-index: 12
,
Michael J. Curtis27
Estimated H-index: 27
,
Michael Williams17
Estimated H-index: 17
Abstract The biomedical research ecosystem—academic, federal, and industrial—is dedicated to understanding human disease causality and developing effective therapeutics. The successes of this system over the past century have resulted in many effective drugs leading society to expect an endless supply of new therapeutics, particularly for chronic diseases that lack effective treatments (e.g., Alzheimer’s, diabetes). An increased recognition that the quality, relevance, reliability, and reproduci...
Published on Jan 1, 2018
Andrea Grignolio4
Estimated H-index: 4
(Sapienza University of Rome)
Data, harsh data, are the only thing that count, we could say, paraphrasing a famous quotation from Stendhal’s The Red and the Black about the truth. At a time like the present, when we are submerged by information by media, it is not at all easy to find our way around to distinguish between what is true and what is false, in the sense of reported untruthfully or partially, or even fictive, in the sense of fake or fabricated. For my generation, which saw it come into being, the Internet has been...
Published on Sep 25, 2017
Published on Jun 1, 2016in Inflammatory Bowel Diseases4.00
Yi Li1
Estimated H-index: 1
(NU: Nanjing University),
Weiming Zhu21
Estimated H-index: 21
(NU: Nanjing University)
+ 1 AuthorsBo Shen49
Estimated H-index: 49
(Cleveland Clinic)
Published on Jan 2, 2015in Expert Review of Vaccines4.53
Eve Dubé14
Estimated H-index: 14
,
Maryline Vivion4
Estimated H-index: 4
,
Noni MacDonald51
Estimated H-index: 51
(Dal: Dalhousie University)
Despite being recognized as one of the most successful public health measures, vaccination is perceived as unsafe and unnecessary by a growing number of parents. Anti-vaccination movements have been implicated in lowered vaccine acceptance rates and in the increase in vaccine-preventable disease outbreaks and epidemics. In this review, we will look at determinants of parental decision-making about vaccination and provide an overview of the history of anti-vaccination movements and its clinical i...
Marta Maia Bosca-Watts3
Estimated H-index: 3
,
Joan Tosca3
Estimated H-index: 3
+ 3 AuthorsFrancisco Mora15
Estimated H-index: 15
The possibility of an infectious origin in inflammatory bowel disease (IBD) has been postulated since the first description of Crohn’s disease (CD). Many observations implicate bacteria as a trigger for the development of CD: lesions occur in regions with higher bacterial concentrations; aphthous ulcers occur in Peyer’s patches; inflammation resolves when the fecal stream is diverted and is reactivated following reinfusion of bowel contents; severity of the disease is correlated with bacterial d...
Published on Dec 1, 2014in BMC Medicine8.29
DimitriosPolymeros14
Estimated H-index: 14
(UoA: National and Kapodistrian University of Athens),
Zacharias P. Tsiamoulos10
Estimated H-index: 10
(UoA: National and Kapodistrian University of Athens)
+ 5 AuthorsSpiros D. Ladas7
Estimated H-index: 7
(UoA: National and Kapodistrian University of Athens)
Background A link between measles virus and Crohn’s disease (CD) has been postulated. We assessed through bioinformatic and immunological approaches whether measles is implicated in CD induction, through molecular mimicry.
View next paperIs measles vaccination a risk factor for inflammatory bowel disease