Match!

Determination of metronidazole in a rat stomach by HPLC for obtaining basic data of the eradication therapy of Helicobacter pylori

Published on Oct 1, 2012in Journal of Pharmaceutical Analysis
· DOI :10.1016/j.jpha.2012.02.007
Mai Kubodera1
Estimated H-index: 1
,
Tadakazu Tokumura1
Estimated H-index: 1
(International University of Health and Welfare),
Yoshiharu Machida38
Estimated H-index: 38
(Hoshi University)
Abstract
Abstract In the eradication therapy of Helicobacter pylori changes of antibiotics as these concentrations or amount in the stomach after oral administration were not clear. A simple and accurate method for determining the concentration of metronidazole (MTZ) in homogenate of rat stomach was developed in order to obtain basic data to design a pharmaceutical preparation having targeting ability to the surface of gastric-mucosa. This method included a deproteinization process by methanol, separation with reversed-phase high-performance liquid chromatography, and detection with an ultraviolet wavelength of 370 nm. Regression analysis showed that the method was linear over a standard curve range from 5 μg/mL to 2000 μg/mL. The inter-day precision and accuracy values between the ranges were 5.0% or better and −7.5 to 5.2%, respectively. The newly developed method was applied to an analysis of gastric samples after oral administration of MTZ at a dose of 5 mg/kg. It was found that the residual MTZ in the stomach was determined within 5 h after dosing. This method is useful for monitoring MTZ in stomach after its oral administration to rats.
  • References (15)
  • Citations (7)
📖 Papers frequently viewed together
1996
8 Authors (Qin Y, ..., Li K)
3 Citations
12 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References15
Newest
#1Abdullahi Musa (ABU: Ahmadu Bello University)H-Index: 4
#2Ia Yakasai (ABU: Ahmadu Bello University)H-Index: 2
Last. C Udekwe (ABU: Ahmadu Bello University)H-Index: 1
view all 5 authors...
The purpose of this study was to evaluate whether the optimum prepara-tions are used for the second-line eradication therapy for Helicobacter pylori (H. pylori) infection in Japan. In the therapy, commercial tablets, which have 250 mg of amoxicil-lin (AMX) and 250 mg of metronidazole (MTZ), are used as antibiotics. The evaluation was performed by dissolution tests and simulations for intragastric drug concentrations based on the dissolution data. The dissolution tests were performed using the pa...
3 Citations
#1Hadir M. Maher (Alexandria University)H-Index: 15
#2Rasha M. Youssef (Alexandria University)H-Index: 10
Last. S.M. El-Bahr (Alexandria University)H-Index: 11
view all 4 authors...
Abstract An efficient multiresidue method for the simultaneous determination of metronidazole (MET) and spiramycin (SPY) in tilapia fish muscle, based on high performance liquid chromatography with UV detection (HPLC-UV), has been developed. The drugs were extracted with 0.2% orthophosphoric acid–methanol (6:4), and the extracts were cleaned up on a solid phase extraction cartridge, C18 Sep-Pak® light column. The LC separation was performed on a RP stainless-steel C-18 analytical column (150 mm ...
48 CitationsSource
胃癌は全世界人口における部位別癌死亡の第二位を占める.近年の研究から,cagA遺伝子を保有するヘリコバクター・ピロリ(ピロリ菌)の持続感染が胃癌発症に決定的な役割を担うことが明らかになってきた.cagA遺伝子産物であるCagAタンパク質はピロリ菌が保有するミクロの注射針(IV型分泌機構)を通して菌体内から胃上皮細胞内へと直接注入される.細胞内に侵入したCagAはSHP-2癌タンパク質に代表される細胞内シグナル伝達分子と特異的に結合しそれらの機能を脱制御することにより,細胞増殖・細胞運動にかかわる多彩な細胞内シグナル伝達系を撹乱する.同時に,CagAは上皮細胞の極性制御を担うPAR1b/MARK2キナーゼと結合し不活化する結果,消化管粘膜構築を崩壊させる.一連のこうした生物活性から,CagAは胃癌発症に深くかかわることが推察されてきた.ごく最近,ピロリ菌CagAを全身性に発現するトランスジェニックマウスにおいて胃癌,小腸癌さらには骨髄性白血病,B細胞リンパ腫が発症することが明らかとなり,CagAが生体内で直接の発癌活性を示す細菌由来の初の癌タンパク質(bacterial oncoprot...
6 CitationsSource
Infection with cagA-positive Helicobacter pylori is associated with gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue (MALT) lymphoma of B cell origin. The cagA-encoded CagA protein is delivered into gastric epithelial cells via the bacterial type IV secretion system and, upon tyrosine phosphorylation by Src family kinases, specifically binds to and aberrantly activates SHP-2 tyrosine phosphatase, a bona fide oncoprotein in human malignancies. CagA also elicits junctional and ...
414 CitationsSource
#1Toshio Fujioka (Oita University)H-Index: 35
#2Aoi Yoshiiwa (Oita University)H-Index: 5
Last. Kazunari Murakami (Oita University)H-Index: 32
view all 5 authors...
In the years that followed the issuance in 2000 of the Guidelines for the Management of Helicobacter pylori Infection in Japan by the Japanese Society for Helicobacter Research (JSHR), a number of major issues arose, including the emergence of H. pylori strains resistant to clarithromycin as part of primary eradication therapy, and this led to the Guidelines being revised by the JSHR in 2003. While the JSHR aimed in the 2000 Guidelines to prepare clinicians for the task ahead in view of H. pylor...
30 CitationsSource
Abstract A simple and rapid stability-indicating HPLC assay procedure has been developed and validated for metronidazole benzoate. The HPLC conditions were as follows, column: Waters Symmetry C8, 5 μm packing, 4.6 mm × 250 mm; detection: UV at 271 nm; injection volume: 20 μl; mobile phase: acetonitrile—0.1% glacial acetic acid in monobasic potassium phosphate (0.01 M) (40:60, v/v); isocratic elution under ambient temperature at 2.0 ml min −1 . The procedure separated metronidazole benzoate and i...
17 CitationsSource
#1Tadashi Shimoyama (Hirosaki University)H-Index: 26
#2Shinsaku Fukuda (Hirosaki University)H-Index: 25
Last. Akihiro Munakata (Hirosaki University)H-Index: 30
view all 5 authors...
Background Eradication of Helicobacter pylori has become a common treatment for several diseases. There is an increase in antibiotic-resistant strains, which causes the failure of eradication. The aim of this study was to investigate the usefulness of metronidazole for the treatment of H. pylori infection in patients who failed eradication therapy.
39 CitationsSource
#1Richard M. Peek (Vandy: Vanderbilt University)H-Index: 65
3 CitationsSource
#1Richard H. Argent (University of Nottingham)H-Index: 16
#2Mark Kidd (Yale University)H-Index: 46
Last. John Atherton (University of Nottingham)H-Index: 64
view all 6 authors...
Abstract Background & Aims: The Helicobacter pylori cag pathogenicity island encodes a secretory system that translocates CagA into epithelial cells, where it becomes tyrosine phosphorylated and induces cytoskeletal rearrangements. Strains with more CagA tyrosine phosphorylation motifs are most closely associated with gastric cancer. Here we assess whether clinical strains can deliver CagA, whether strains with different numbers of CagA phosphorylation motifs have CagA phosphorylated to differen...
172 CitationsSource
#1Hirokazu Katayama (Fukuyama University)H-Index: 13
#2Takeya Nishimura (Fukuyama University)H-Index: 1
Last. Hironori Yoshitomi (Fukuyama University)H-Index: 10
view all 7 authors...
We prepared a new liquid preparation for eradication of Helicobacter pylori (HP), and examined drug release in vitro and in vivo. The liquid preparation mainly consisted of a sodium alginate (AG) aqueous solution containing ampicillin (ABPC), an antibiotic drug, or methylene blue, a dye. Drug release was retarded by Ca pre-treatment (0.10 M, 20 s) of the AG preparation in in vitro drug release studies due to gel-formation at the liquid surface. In in vivo experiments, the AG preparations were ad...
48 CitationsSource
Cited By7
Newest
#1Pravin G. Vanol (Gujarat University)H-Index: 1
#2Mallika Sanyal (Gujarat University)H-Index: 2
Last. Pranav S. Shrivastav (St. Xavier's College-Autonomous, Mumbai)H-Index: 1
view all 4 authors...
1 CitationsSource
#1Pravin G. Vanol (St. Xavier's College-Autonomous, Mumbai)H-Index: 2
#2Mallika Sanyal (St. Xavier's College-Autonomous, Mumbai)H-Index: 17
Last. Pranav S. Shrivastav (Gujarat University)H-Index: 23
view all 4 authors...
5 CitationsSource
#1Martha M. Morcoss (Nahda University)H-Index: 1
#2Nada S. Abdelwahab (Beni-Suef University)H-Index: 8
Last. Mohammed T. Elsaady (Beni-Suef University)H-Index: 2
view all 4 authors...
TLC densitometric and RP-HPLC methods are innovative chromatographic methods used for determination of diloxanide furoate, metronidazole and its impurity, 4-nitroimidazole. In the developed TLC densitometric method, appropriate separation was achieved using silica gel 60 F254 TLC plates and ethyl acetate/acetone/hexane/ammonia solution (9.5:0.5:0.3:0.3, by volume), as a developing system and the separated bands were UV-scanned at 276 nm. While the developed RP-HPLC method depended on separation ...
1 CitationsSource
#1Gamal M. HamadH-Index: 2
#2Tarek H. TahaH-Index: 5
Last. Ali Alshehri (King Khalid University)H-Index: 6
view all 4 authors...
Helicobacter pylori is a common human pathogen infecting about 30 % of children and 60 % of adults world- wide. It is responsible for diseases such as gastritis, peptic ulcer and gastric cancer. H. pylori treatment based on antibi- otics with proton pump inhibitor, but therapy failure is shown to be higher than 20 % and is essentially due to an increasing in prevalence of antibiotic-resistant bacteria, which has led to the search for alternative therapies. In this study, we discuss the usage of ...
6 CitationsSource
#1Mai KuboderaH-Index: 1
#2Tadakazu Tokumura (Tokushima Bunri University)H-Index: 1
Last. Yoshiharu MachidaH-Index: 5
view all 3 authors...
In the eradication therapy of Helicobacter pylori, the changes of the amounts and concentrations of antibiotics in the stomach after oral administration were unclear. Amoxicillin (AMX) and metronidazole (MTZ), which are used for such therapy, were selected for this study. The levels of these drugs in homogenate of the stomach after their oral administration to rats were determined by HPLC. The doses of AMX and MTZ to the rats were 15 mg/kg and 5 mg/kg, respectively. The proportions of these drug...
#1Y. Bahari Mollamahale (Sharif University of Technology)H-Index: 4
#2Mohammad Ghorbani (Sharif University of Technology)H-Index: 26
Last. Abolghasem Dolati (Sharif University of Technology)H-Index: 22
view all 5 authors...
Abstract Three-dimensional gold nanoelectrode ensembles (3D GNE) have proven to be promising nanoelectrodes by representing much higher sensitivity compared to both their 2D nanostructures and bulk counterparts. The sensitivity of 3D gold nanotubes (GNTs) fabricated through electrodeposition inside the pores of polycarbonate templates, was examined toward metronidazole (MTZ) as one of their pharmaceutical applications. The electrochemical behavior of MTZ at the 3D GNT-modified electrode was disc...
32 CitationsSource
#1Asigul Muhammad (XJU: Xinjiang University)H-Index: 1
#2Turghun Muhammad (XJU: Xinjiang University)H-Index: 9
Last. Burabiye Yakup (XJU: Xinjiang University)H-Index: 2
view all 4 authors...
In the present paper, we report the fabrication of a new sensing membrane for fluorescence detection of metronidazole (MNZ). Briefly, a pyrenebutyric acid derivative, 2-(methacryloyloxy) ethyl-4-(1-pyrenyl) butanoate (MPB) with a double bond, was synthesized and copolymerized with 2-hydroxyethylmethacrylate (HEMA) on the activated glass surface by thermal initiation in the presence of cross-linker. The sensor responds linearly to metronidazole in the concentration range of 1.23~35.48 mg.L−1 in a...
6 CitationsSource