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Drop of egg production in chickens by experimental infection with an avian metapneumovirus strain PLE8T1 derived from swollen head syndrome and the application to evaluate vaccine

Published on Jan 1, 2006in Journal of Veterinary Medical Science0.91
· DOI :10.1292/jvms.68.783
Miki Sugiyama2
Estimated H-index: 2
(Merial),
Hiroyuki Koimaru1
Estimated H-index: 1
(Merial)
+ 3 AuthorsToshihiro Ito25
Estimated H-index: 25
(Tottori University)
Abstract
Decreases in egg production and increased incidence of abnormal eggs due to malformation of egg shells were observed in specific pathogen free (SPF) 173-day-old laying hens inoculated intravenously with an avian metapneumovirus (aMPV) strain PLE8T1. This strain was derived from an isolate from broiler birds exhibiting swollen head syndrome (SHS). Some SPF birds inoculated with the virus showed, slight diarrhea without any respiratory symptoms. Thus, the PLE8T1 strain was used as a challenge virus to evaluate efficacy of aMPV vaccines. SPF chickens which received a live attenuated aMPV vaccine (NEMOVAC; Merial) at 7 or 77 days old and an inactivated aMPV vaccine (OVO-4; Merial) at 105 days old were protected against poor egg production caused by the challenge with the PLE8T1 strain. Thus, aMPV, the PLE8T1 strain passaged 22 times after isolation, from birds exhibiting SHS, could induce a drop in egg production in laying hens accompanied by malformation of egg shells. It was suggested that this challenge system could be applied to evaluate the efficacy of aMPV vaccine.
  • References (16)
  • Citations (13)
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References16
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#1Jane K. A. CookH-Index: 31
#2J. ChesherH-Index: 3
Last. M. B. HugginsH-Index: 18
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Administration of a virulent strain of avian pneumovirus (APV) to specific pathogen free laying hens by the oculonasal route failed to induce a drop in egg production or any adverse effects on eggshell quality. However, intravenous (i.v.) inoculation of the same strain caused a substantial drop in egg production and a high incidence of soft and thin-shelled eggs. Some respiratory signs were also observed and the hens appeared sick, with diarrhoea being observed in approximately one-half of the h...
17 CitationsSource
Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99·7% nt and 99·0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70·0–80·5% and 77·6–97·2%, respectively, with the L gene sharing 76·1% nt and 85·3% aa identity with that of a...
136 CitationsSource
#1Elena CatelliH-Index: 13
#2Jane K. A. CookH-Index: 31
Last. M. B. HugginsH-Index: 18
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A subgroup B isolate of turkey rhinotracheitis virus (TRTV) or avian pneumovirus (APV), obtained from a flock of commercial breeding chickens experiencing poor egg production, mortality and swollen head syndrome, was shown to cause substantial respiratory signs in both young SPF chickens and chicks with high levels of maternally derived TRT antibodies. This isolate replicated to high titre in the respiratory tract of experimentally inoculated SPF chickens for approximately 5 days after inoculati...
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Three commercial ELISAs (Pathasure, Svanovir and Flockscreen) were compared in their ability to detect turkey rhinotracheitis virus (TRTV) vaccine‐induced antibodies. Sequential sera taken from specified pathogen‐free chickens, vaccinated with two combinations of live and inactivated TRTV vaccines were used. The vaccines were based on TRTV strains from the United Kingdom (Intervet) and from France (Rhone Merieux). One ELISA failed to detect antibodies after live vaccination with both French and ...
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#1Kikuyasu NakamuraH-Index: 23
#2Masaji MaseH-Index: 27
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Attempts to reproduce swollen head syndrome (SHS) lesions were carried out in specific pathogen‐free (SPF) chickens. In Experiment 1, chickens inoculated into the submucosal tissue of the nasal membrane or subcutaneous tissue of eyelids with four different strains of Escherichia coli, developed typical SHS lesions; purulent and necrotic lesions of facial subcutis (especially around their eyelids), periocular connective tissue, infraorbital sinus, air spaces, middle ears and eyeballs. The lesions...
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#1Jane K. A. CookH-Index: 31
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Summary The experimental inoculation of 38‐week‐old turkey hens with a pool of field isolates of turkey rhinotracheitis virus (TRTV) induced a marked respiratory infection and a substantial drop in egg production. Administration of a live‐attenuated TRT vaccine at 1 week of age did not protect the layers against respiratory infection, but provided good protection against the effects of challenge on laying performance. However, a combination of live priming followed by injection of inactivated va...
34 CitationsSource
Turkey rhinotracheitis (TRT) virus was first isolated from a commercial broiler chicken with swollen head syndrome (SHS) in Japan. At the same time, Newcastle disease virus (NDV), infectious bronchitis virus (IBV), avian reovirus (ARV), Escherichia coli (E.coli), Morganella morganii, and Proteus mirabilis were also isolated from the same broiler Chicken. The presence of antibodies to TRT virus was confirmed in the sera of 34-day-old chickens of the flock with SHS, however the antibodies to TRT v...
42 CitationsSource
#1Natàlia MajóH-Index: 20
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SUMMARY. The histopathologic changes and the distribution of turkey rhinotracheitis virus (TRTV) antigen in the respiratory and reproductive tracts of experimentally infected chickens, turkey poults, and broiler breeders is described. TRTV antigen was detected using both immunofluorescent staining of cryostat sections and immunoperoxidase staining of formalin-fixed tissues. Viral antigen was observed associated with the cilia of the epithelial cells of turbinates, trachea, and lung. No TRTV anti...
33 CitationsSource
#1Y. S. LuH-Index: 1
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Summary Outbreaks of swollen head syndrome (SHS) were observed in two broiler and two broiler‐breeder farms in Taiwan. The disease was characterized by oedematous swelling of the head, especially surrounding the eyelids, the neck and wattles. Avian pneumovirus and Escherichia coli were isolated from birds in all four farms. In addition, Staphylococcus aureus and infectious bursal disease virus were each isolated from one farm. A serological survey of 398 birds from 11 broiler breeder farms showe...
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#1M. PattisonH-Index: 1
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Chickens on 14 broiler breeder farms were examined at various times throughout their laying cycle. Antibodies to the turkey rhinotracheitis virus were common although they were not always accompanied by clinical signs of the swollen head syndrome. Ninety-nine broiler flocks were tested of which only 20 were serologically positive to the virus. Some of these infections were subclinical. On nine farms where swollen head syndrome occurred several successive flocks were sampled; the syndrome occurre...
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