Roles of specialized DNA polymerases in mutagenesis by oxidized guanine

To assess the involvement of specialized DNA polymerases in the mutagenesis induced by an oxidized form of dGTP, 8-hydroxy-2′-deoxyguanosine 5′-triphosphate (8-OH-dGTP, 7,8-dihydro-8-oxo-2′-deoxyguanosine 5′-triphosphate), shuttle plasmid DNA containing the supF gene and 8-OH-dGTP were introduced into human 293T cells in which specialized DNA polymerases were knocked-down by siRNAs. The knock-downs of DNA polymerases η and ζ, and REV1 reduced...