Lipidic cubic phases as matrices for membrane protein crystallization
Abstract
This review provides detailed procedures for the crystallization of membrane proteins via the lipidic cubic phase method. Bacteriorhodopsin-specific, hands-on protocols are given for (i) the preparation of bacteriohordopsin from purple membrane by monomerization in octylglucoside and gel filtration chromatography or by selective extraction after pre-treatment with dodecyl-trimethylammonium bromide, (ii) the incorporation of bacteriorhodopsin...
Paper Details
Title
Lipidic cubic phases as matrices for membrane protein crystallization
Published Date
Nov 1, 2004
Journal
Volume
34
Issue
3
Pages
348 - 353
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