Comparison of a β-Glucosidase and a β-Mannosidase from the Hyperthermophilic Archaeon Pyrococcus furiosus

Two distinct exo-acting, β-specific glycosyl hydrolases were purified to homogeneity from crude cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a β-glucosidase, corresponding to the one previously purified by Kengen et al. (Kengen, S. W. M., Luesink, E. J., Stams, A. J. M., and Zehnder, A. J. B. (1993) Eur. J. Biochem. 213, 305-312), and a β-mannosidase. The β-mannosidase and β-glucosidase genes were isolated from a genomic...