Rapid and simultaneous determination of hair polyamines as N-heptafluorobutyryl derivatives by gas chromatography–mass spectrometry

We have developed a simple and sensitive method for the simultaneous determination of putrescine, spermidine and spermine in hair as their N-heptafluorobutyryl derivatives by gas chromatography-mass spectrometry (GC-MS) in selected ion-monitoring (SIM) mode. After base hydrolysis, hair samples were extracted with solid-phase extraction (SPE) with diatomaceous earth columns, followed by derivatization with heptafluorobutyryl chloride (HFB-Cl) and...