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Detection of enteric pathogens in Turkey flocks affected with severe enteritis, in Brazil

Published on Aug 1, 2014in Tropical Animal Health and Production 1.09
· DOI :10.1007/s11250-014-0612-7
Joelma Moura-Alvarez2
Estimated H-index: 2
(USP: University of São Paulo),
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo)
+ 5 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
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Abstract
Twenty-two flocks of turkeys affected by enteric problems, with ages between 10 and 104 days and located in the Southern region of Brazil, were surveyed for turkey by PCR for turkey astrovirus type 2 (TAstV-2), turkey coronavirus (TCoV), hemorrhagic enteritis virus (HEV), rotavirus, reovirus, Salmonella spp., and Lawsonia intracellularis (Li) infections. Eleven profiles of pathogen combination were observed. The most frequently encountered pathogen combinations were TCoV-Li, followed by TCoV-TAstV-2-Li, TCoV-TastV-2. Only TCoV was detected as the sole pathogen in three flocks. Eight and 19 flocks of the 22 were positive for TAstV-2 and TCoV, respectively. Six were positive for Salmonella spp. and L. intracellularis was detected in 12 turkey flocks. Reovirus and HEV were not detected in this survey. These results throw new light on the multiple etiology of enteritis in turkeys. The implications of these findings and their correlation with the clinical signs are comprehensively discussed, illustrating the complexity of the enteric diseases.
  • References (40)
  • Citations (12)
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References40
Newest
Published on Aug 6, 2013in Avian Pathology 1.96
Sunil K. Mor11
Estimated H-index: 11
(UMN: University of Minnesota),
Tamer A. Sharafeldin5
Estimated H-index: 5
(UMN: University of Minnesota)
+ 4 AuthorsDevi P. Patnayak16
Estimated H-index: 16
(UMN: University of Minnesota)
Two studies were conducted to determine the role of enteric viruses in Light Turkey Syndrome (LTS), which is characterized by lower weight in market age turkeys than their standard breed character. In the surveillance study, we selected four LTS and two non-LTS turkey flocks in Minnesota and collected faecal samples at 2, 3, 5 and 8-weeks of age. Astrovirus, rotavirus, and reovirus were detected alone or in various combinations in both LTS and non-LTS flocks. No coronavirus was detected in LTS f...
Published on Nov 1, 2012in Journal of Virological Methods 1.75
Delana Andreza Melo Bezerra4
Estimated H-index: 4
,
René Ribeiro da Silva3
Estimated H-index: 3
(Ministry of Agriculture)
+ 5 AuthorsJoana D'Arc Pereira Mascarenhas23
Estimated H-index: 23
Abstract Group D rotaviruses (RVs-D) have been documented in birds and, while they may be common in these animals, few molecular studies are available for this specific group. In this study, specific primers for the gene that encodes for the RVs-D VP6 protein were designed and used in a reverse transcription polymerase chain reaction (RT-PCR). Thirty pools of samples were tested by polyacrylamide gel electrophoresis (PAGE) yielding a 30% (9/30) positivity. These pools were subjected subsequently...
Published on Sep 1, 2012in Avian Diseases 1.31
Naresh Jindal10
Estimated H-index: 10
(UMN: University of Minnesota),
Yogesh Chander17
Estimated H-index: 17
(UMN: University of Minnesota)
+ 3 AuthorsSagar M. Goyal49
Estimated H-index: 49
(UMN: University of Minnesota)
SUMMARY. This study was undertaken to develop and validate a multiplex reverse transcription-polymerase chain reaction (mRT-PCR) for simultaneous detection of avian rotavirus, turkey astrovirus-2 (TAstV-2), and avian reovirus. Primers targeting the conserved regions of NSP4 gene of avian rotavirus, polymerase gene of TAstV-2, and S4 gene of avian reovirus were used. The position of bands at 630, 802, and 1120 base pairs on agarose gel confirmed the presence of rotavirus, TAstV-2, and reovirus, r...
Published on Jun 1, 2010in Veterinary Journal 1.92
Magdalena Jacobson13
Estimated H-index: 13
(SLU: Swedish University of Agricultural Sciences),
Claes Fellström24
Estimated H-index: 24
(SLU: Swedish University of Agricultural Sciences),
Marianne Jensen-Waern21
Estimated H-index: 21
(SLU: Swedish University of Agricultural Sciences)
Abstract Proliferative enteropathy caused by the intracellular bacterium Lawsonia intracellularis is an endemic disease with high herd prevalences reported worldwide. The infection has a considerable impact on pig production and herd economics and, with the development of new diagnostic techniques, L. intracellularis is being identified in an increasing number of pig herds and a wider range of species. This paper reviews current knowledge of the disease, with a focus on the epidemiology in pigs....
Published on Sep 1, 2009in Avian Diseases 1.31
Amarilis Novaes D'Elboux Bunger1
Estimated H-index: 1
(USP: University of São Paulo),
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo)
+ 1 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Abstract Turkey coronavirus (TCoV) is a causative agent associated with poult enteritis and mortality syndrome (PEMS) in turkeys worldwide. The disease is an acute, highly contagious enteric disease that is characterized by depression, anorexia, diarrhea, and high mortality in commercial turkey flocks. The presence of TCoV in 12 intestinal-content samples, from turkey flocks aged between 10 and 104 days and exhibiting severe enteritis, was monitored during the period of 2004 to 2006. TCoV detect...
Published on Jun 1, 2009in Avian Diseases 1.31
Naresh Jindal10
Estimated H-index: 10
(UMN: University of Minnesota),
Devi P. Patnayak16
Estimated H-index: 16
+ 2 AuthorsSagar M. Goyal49
Estimated H-index: 49
Abstract A retrospective study was conducted to determine the occurrence of poult enteritis syndrome (PES) in Minnesota from January 2002 to December 2007. PES is an infectious intestinal disease of young turkeys between 1 day and 7 wk of age and is characterized by diarrhea, depression, and lethargy with pale intestines and/or excessively fluid cecal contents. During the study period, samples from 1736 turkey flocks were submitted to the Minnesota Veterinary Diagnostic Laboratory for disease in...
Published on Jun 1, 2009in Brazilian Journal of Microbiology 2.86
S.E.L. da Silva1
Estimated H-index: 1
(UFU: Federal University of Uberlandia),
Ana Maria Bonetti11
Estimated H-index: 11
(UFU: Federal University of Uberlandia)
+ 3 AuthorsTereza C. Cardoso13
Estimated H-index: 13
(UNESP: Sao Paulo State University)
A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileumcaeca region were collected from 30-day-old poults suffering of enteriti...
Published on Jan 1, 2009in Journal of Wildlife Diseases 1.15
Marilyn G. Spalding22
Estimated H-index: 22
The 12th edition of Diseases of Poultry is a ‘‘must have’’ reference for those involved in avian diagnostics, and it continues to be a basic foundation for those engaged in avian disease research. First published in 1943, Diseases of Poultry has been reissued every four to seven years. I started with the 8th edition and have never questioned my purchase of each subsequent edition. Over 65 years of publication, the editors, contributors, and chapters have been refined, reshuffled, added to, and e...
Published on Jun 1, 2008in Avian Diseases 1.31
Mary J. Pantin-Jackwood33
Estimated H-index: 33
(ARS: Agricultural Research Service),
J. Michael Day5
Estimated H-index: 5
(ARS: Agricultural Research Service)
+ 1 AuthorsErica Spackman39
Estimated H-index: 39
(ARS: Agricultural Research Service)
Abstract Intestinal samples collected from 43 commercial broiler and 33 commercial turkey flocks from all regions of the United States during 2005 and 2006 were examined for the presence of astrovirus, rotavirus, reovirus, and coronavirus by reverse transcription-polymerase chain reaction (PCR), and for the presence of groups 1 and 2 adenovirus by PCR. Phylogenetic analysis was performed to further characterize the viruses and to evaluate species association and geographic patterns. Astroviruses...
Published on Sep 1, 2007in Avian Diseases 1.31
J. Michael Day5
Estimated H-index: 5
(ARS: Agricultural Research Service),
Erica Spackman39
Estimated H-index: 39
(ARS: Agricultural Research Service),
Mary J. Pantin-Jackwood33
Estimated H-index: 33
(ARS: Agricultural Research Service)
Abstract Recent studies have revealed the presence of astroviruses and rotavirus in numerous poorly performing and healthy chicken and turkey flocks in the United States. The phylogenetic analysis of the sequence data produced during these studies has identified four groups of avian astroviruses circulating in the United States: turkey astrovirus types 1 and 2 (TAstV-1 and TAstV-2), avian nephritis virus (ANV), and a chicken-origin astrovirus (CAstV). As the molecular epidemiology of poultry ent...
Cited By12
Newest
Published on Jul 1, 2019in Veterinary Immunology and Immunopathology 1.85
Andreia T. Marques3
Estimated H-index: 3
(University of Milan),
Sandra I. Anjo11
Estimated H-index: 11
(UC: University of Coimbra)
+ 5 AuthorsFabrizio Ceciliani26
Estimated H-index: 26
(University of Milan)
Abstract Haemorrhagic enteritis (HE) is a viral disease affecting intestinal integrity and barrier function in turkey ( Meleagris gallopavo ) and resulting in a significant economic loss. Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH-MS) was applied to identify crucial proteins involved in HE infection. A total of 938 proteins were identified and used to generate a reference library for SWATH-MS analysis. In total, 523 proteins were reliably quantified, and ...
Published on Oct 1, 2018in Poultry Science 2.03
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo),
Silvana Hipatia Santander Parra5
Estimated H-index: 5
(USP: University of São Paulo)
+ 5 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Published on Sep 11, 2018in PLOS ONE 2.78
Christina McCowan11
Estimated H-index: 11
,
Sandra Crameri11
Estimated H-index: 11
(AAHL: Australian Animal Health Laboratory)
+ 12 AuthorsJenni Harper1
Estimated H-index: 1
(AAHL: Australian Animal Health Laboratory)
Cases of vomiting and diarrhoea were reported in racing pigeons in Western Australia in May, 2016. Morbidity and mortality rates were high. Similar clinical disease was seen in Victoria in December and by early 2017 had been reported in all states except the Northern Territory, in different classes of domestic pigeon–racing, fancy and meat bird–and in a flock of feral pigeons. Autopsy findings were frequently unremarkable; histological examination demonstrated significant hepatic necrosis as the...
Published on Oct 1, 2017in Poultry Science 2.03
Sara D’Andreano2
Estimated H-index: 2
(Autonomous University of Barcelona),
Armand Sànchez Bonastre2
Estimated H-index: 2
(Autonomous University of Barcelona)
+ 5 AuthorsFabrizio Ceciliani26
Estimated H-index: 26
(University of Milan)
Published on Feb 1, 2017in Journal of Comparative Pathology 1.25
T. Ohta1
Estimated H-index: 1
,
K. Kimura1
Estimated H-index: 1
(National Agriculture and Food Research Organization)
+ 4 AuthorsTakashi Onodera26
Estimated H-index: 26
(UTokyo: University of Tokyo)
Summary Proliferative enteropathy (PE) is an infectious disease caused by Lawsonia intracellularis (Li), an obligate intracellular bacterium. PE is endemic in swine herds and has been reported in a variety of mammals including horses, hamsters, rabbits, rats, guinea pigs, ferrets, foxes, dogs, sheep, deer and non-human primates. Avian cases are reported only in ratite birds, including emus and ostriches. Some studies show an absence of Lawsonia spp. infection in chickens. In this study, we perfo...
Published on Jan 1, 2017in Veterinary Quarterly 2.34
Kuldeep Dhama24
Estimated H-index: 24
(IVRI: Indian Veterinary Research Institute),
Vasudevan Gowthaman1
Estimated H-index: 1
(Veterinary College, Mathura)
+ 7 AuthorsMuhammad Munir17
Estimated H-index: 17
ABSTRACTHaemorrhagic enteritis virus (HEV), an adenovirus associated with acute haemorrhagic gastro-intestinal disease of 6–11-week old turkeys predominantly hampers both humoral and cellular immunity. Affected birds are more prone to secondary complications (e.g. colibacillosis and clostridiosis) and failure to mount an effective vaccine-induced immune response. HEV belongs to the new genus Siadenovirus. Feco-oral transmission is the main route of entry of the virus and it mainly colonizes burs...
Published on Sep 1, 2016in Avian Diseases 1.31
Luis Ramiro Luna Espinoza2
Estimated H-index: 2
(USP: University of São Paulo),
Laila Andreia Rodrigues Beserra2
Estimated H-index: 2
(USP: University of São Paulo)
+ 1 AuthorsFabio Gregori10
Estimated H-index: 10
(USP: University of São Paulo)
SUMMARY Astrovirus is a common cause of enteritis in humans and domestic animals. Here we report the detection of turkey astrovirus type 1 (TAstV-1) and chicken astrovirus (CAstV) in avian farms. Sixty fecal sample pools (five or six birds of the same flock), from chickens without apparent clinical symptoms of enteric disease from farms located in six Brazilian states, were screened by an ORF1b PCR, followed by nucleotide sequencing of amplified products and phylogenetic analysis. Six samples te...
Published on Jul 1, 2016in Pesquisa Veterinaria Brasileira 0.30
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo),
Silvana Hipatia Santander Parra5
Estimated H-index: 5
(USP: University of São Paulo)
+ 4 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Enteric disease is a multifactorial problem in chickens, which causes gastrointestinal alterations, elevated feed conversions and impairment. In the last years, several enteric viruses were implicated in enteric disease; case reports have shown their presence alone or in concomitant infections during outbreaks and have suggested that they might be determining factors in the aetiology of enteric disease. This study shows high detection rates of enteric viruses in the pancreas and spleen in sample...
Published on Mar 1, 2016in Avian Diseases 1.31
Aline Padilha Fraga3
Estimated H-index: 3
(ULBRA: Universidade Luterana do Brasil),
Nilo Ikuta16
Estimated H-index: 16
(ULBRA: Universidade Luterana do Brasil)
+ 5 AuthorsVagner Ricardo Lunge12
Estimated H-index: 12
(ULBRA: Universidade Luterana do Brasil)
SUMMARY The avian infectious bronchitis virus is classified into serotypes or genotypes (or both) in different poultry-producing countries of the world. In Brazil, Massachusetts type (Mass), used as a live vaccine, and local field Brazilian variants (genotypes; BR) predominate in the commercial poultry flocks. This study describes the development and validation of two real-time reverse-transcription polymerase chain reactions (RT-qPCR) for the specific detection of Mass and BR genotypes in allan...
Published on Nov 2, 2015in Avian Pathology 1.96
Emiliana Falcone15
Estimated H-index: 15
(ISS: Istituto Superiore di Sanità),
Chiara Busi2
Estimated H-index: 2
+ 6 AuthorsMaria Beatrice Boniotti11
Estimated H-index: 11
Avian rotaviruses are still largely undefined despite being widespread in several avian species and despite the economic impact of rotavirus (RV) enteritis in poultry flocks. In this study, the presence of different avian RV groups was investigated in commercial poultry flocks reared in Northern and Central Italy and with a history of enteric diseases. Faeces or intestinal contents from different avian species previously found to contain RV particles by electron microscopy (EM) were analysed by ...