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Genetic characterization of avian metapneumovirus subtype C isolated from pheasants in a live bird market

Published on Sep 1, 2007in Virus Research2.74
· DOI :10.1016/j.virusres.2007.03.029
Eun Ho Lee5
Estimated H-index: 5
(CBNU: Chungbuk National University),
Min-Suk Song24
Estimated H-index: 24
(CBNU: Chungbuk National University)
+ 5 AuthorsYoung Ki Choi29
Estimated H-index: 29
(CBNU: Chungbuk National University)
Cite
Abstract
Abstract Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in experimentally re-infected pheasants was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR). Chickens and mice were experimentally inoculated with PL-1 to test the replication potential of PL-1 in other species. Although one specimen from the nasal turbinates of an inoculated chicken showed a slight trace of viral replication at 3 days post-infection (dpi), all of the infected mice were negative for aMPV by RT-PCR throughout the experiment, suggesting that PL-1 does not readily infect mammals. This is the first report of the isolation and complete genomic sequence of aMPV subtype C originating from pheasants.
  • References (35)
  • Citations (37)
Cite
References35
Newest
Published on Jul 15, 2005in Journal of Virology4.32
Marie-Ève Hamelin22
Estimated H-index: 22
,
Kevin C. Yim11
Estimated H-index: 11
+ 5 AuthorsGuy Boivin63
Estimated H-index: 63
Human metapneumovirus (hMPV) is a newly described member of the Paramyxoviridae family causing acute respiratory tract infections, especially in young children. We studied the pathogenesis of this viral infection in two experimental small animal models (BALB/c mice and cotton rats). Significant viral replication in the lungs of both animals was found following an intranasal challenge of 108 50% tissue culture infectious doses (TCID50) and persisted for <2 and <3 weeks in the case of cotton rats ...
Published on May 1, 2005in Virus Genes1.62
Dhanasekaran Govindarajan7
Estimated H-index: 7
(Virginia–Maryland Regional College of Veterinary Medicine),
Siba K. Samal35
Estimated H-index: 35
(Virginia–Maryland Regional College of Veterinary Medicine)
Published on Jan 1, 2005in Virus Research2.74
Humphrey C. M. Lwamba6
Estimated H-index: 6
(UMN: University of Minnesota),
Rene Alvarez19
Estimated H-index: 19
+ 4 AuthorsBruce S. Seal37
Estimated H-index: 37
(ARS: Agricultural Research Service)
Abstract We determined the nucleotide (nt) sequence of the small hydrophobic (SH), attachment glycoprotein (G), and RNA polymerase (L) genes, plus the leader and trailer regions of the Colorado strain of Avian metapneumovirus subtype C (aMPV/C) in order to complete the genome sequencing. The complete genome comprised of 13,134 nucleotides, with a 40 nt leader at its 3′ end and a 45 nt trailer at its 5′ end. The aMPV/C L gene was the largest with 6173 nt and consisting of a single open reading fr...
Published on Dec 15, 2004in Journal of Virology4.32
Rene Alvarez19
Estimated H-index: 19
,
Kevin S. Harrod24
Estimated H-index: 24
+ 2 AuthorsRalph A. Tripp55
Estimated H-index: 55
Human metapneumovirus (HMPV) has emerged as an important human respiratory pathogen causing upper and lower respiratory tract infections in young children and older adults. Recent epidemiological evidence indicates that HMPV may cocirculate with respiratory syncytial virus, and HMPV infection has been associated with other respiratory diseases. In this study, we show that BALB/c mice are susceptible to HMPV infection, the virus replicates in the lungs with biphasic growth kinetics in which peak ...
Published on Dec 1, 2004in Avian Diseases1.31
Richard S. Bennett5
Estimated H-index: 5
(UMN: University of Minnesota),
Jill Nezworski1
Estimated H-index: 1
(UMN: University of Minnesota)
+ 7 AuthorsDavid A. Halvorson27
Estimated H-index: 27
(UMN: University of Minnesota)
Resumen RESUMEN. Nota de Investigacion—Evidencia de la difusion del pneumovirus aviar entre aves salvajes y domesticas desde el estado de Minnesota a la zona central de Norte America. Se examinaron tejidos de turbinas nasales y hendiduras palatinas de pavos domesticos y aves salvajes para determinar la presencia del pneumovirus aviar en la zona central de Norte America mediante la prueba de la transcriptasa reversa-reaccion en cadena por la polimerasa (RT-PCR). Se determino la presencia de antic...
Published on Aug 1, 2004in Virus Research2.74
Mark G. Wise5
Estimated H-index: 5
(ARS: Agricultural Research Service),
Holly S Sellers4
Estimated H-index: 4
(ARS: Agricultural Research Service)
+ 1 AuthorsBruce S. Seal37
Estimated H-index: 37
(ARS: Agricultural Research Service)
Abstract Nucleotide sequence was determined for the RNA-dependent RNA polymerase (L) gene of 16 Newcastle disease virus (NDV) isolates from diverse geographic and chronological origins. The observed consensus amino acid sequence conformed to the six domains previously identified among paramyxovirus L proteins, and the putative 749 QGDNQ 753 active site was strictly conserved among all isolates. Analysis of predicted amino acid sequences allowed us to identify a sequencing error in the previously...
Published on Jun 1, 2004in Journal of General Virology2.81
Mia MacPhail10
Estimated H-index: 10
(MedImmune),
Jeanne H. Schickli10
Estimated H-index: 10
(MedImmune)
+ 6 AuthorsAurelia Haller8
Estimated H-index: 8
(MedImmune)
Human metapneumovirus (hMPV), a recently identified paramyxovirus, is the causative agent of respiratory tract disease in young children. Epidemiological studies have established the presence of hMPV in retrospective as well as current clinical samples in Europe, USA, Canada, Hong Kong and Australia. The hMPV disease incidence rate varied from 7 to 12%. This rate of disease attack places hMPV in severity between respiratory syncytial virus and human parainfluenza virus type 3, two common respira...
Published on Jun 1, 2004in American Journal of Pathology3.76
Thijs Kuiken58
Estimated H-index: 58
(EUR: Erasmus University Rotterdam),
Bernadette G. van den Hoogen27
Estimated H-index: 27
(EUR: Erasmus University Rotterdam)
+ 5 AuthorsAlbert D. M. E. Osterhaus112
Estimated H-index: 112
(EUR: Erasmus University Rotterdam)
A substantial proportion of hitherto unexplained respiratory tract illnesses is associated with human metapneumovirus (hMPV) infection. This virus also was found in patients with severe acute respiratory syndrome (SARS). To determine the dynamics and associated lesions of hMPV infection, six cynomolgus macaques (Macaca fascicularis) were inoculated with hMPV and examined by pathological and virological assays. They were euthanized at 5 (n = 2) or 9 (n = 2) days post-infection (dpi), or monitored...
Published on Aug 1, 2003in Journal of General Virology2.81
D. Toquin19
Estimated H-index: 19
,
C. de Boisséson5
Estimated H-index: 5
+ 2 AuthorsN. Eterradossi23
Estimated H-index: 23
The genes encoding the putative small hydrophobic (SH), attachment (G) and polymerase (L) proteins of the Colorado isolate of subgroup C avian pneumovirus (APV) were entirely or partially sequenced. They all included metapneumovirus (MPV)-like gene start and gene end sequences. The deduced Colorado SH protein shared 26·9 and 21·7 % aa identity with its counterpart in human MPV (hMPV) and APV subgroup A, respectively, but its only significant aa similarities were to hMPV. Conserved features inclu...
Published on May 1, 2003in Virus Research2.74
Abdul S. Yunus10
Estimated H-index: 10
(Virginia–Maryland Regional College of Veterinary Medicine),
Dhanasekaran Govindarajan7
Estimated H-index: 7
(Virginia–Maryland Regional College of Veterinary Medicine)
+ 1 AuthorsSiba K. Samal35
Estimated H-index: 35
(Virginia–Maryland Regional College of Veterinary Medicine)
Abstract We report here the nucleotide and deduced amino acid (aa) sequences of the small hydrophobic (SH) gene of the avian pneumovirus strain Colorado (APV/CO). The SH gene of APV/CO is 628 nucleotides in length from gene-start to gene-end. The longest ORF of the SH gene encoded a protein of 177 aas in length. Comparison of the deduced aa sequence of the SH protein of APV/CO with the corresponding published sequences of other members of genera metapneumovirus showed 28% identity with the newly...
Cited By37
Newest
Published on Mar 1, 2019in Pesquisa Veterinaria Brasileira0.30
Laís S. Rizotto1
Estimated H-index: 1
(USP: University of São Paulo),
Raphael M. Simão1
Estimated H-index: 1
(USP: University of São Paulo)
+ 5 AuthorsHelena Lage Ferreira10
Estimated H-index: 10
(USP: University of São Paulo)
Published on Aug 27, 2018in Avian Diseases1.31
E. Bayraktar (CEVA Logistics), S. Umar (Istanbul University)+ 7 AuthorsHuseyin Yilmaz13
Estimated H-index: 13
(Istanbul University)
Viral respiratory diseases including avian metapneumovirus (aMPV) have a significant economic impact on poultry industries. The frequency and genotype diversity of aMPV in Turkish broiler flocks is not known at present. The aim of this study was to report the first molecular identification and phylogeny of aMPV which is circulating in Turkish broiler flocks. Trachea tissue samples and tracheal swabs were collected from 110 broiler flocks distributed in different geographical regions in Turkey be...
Published on Aug 1, 2018in Transboundary and Emerging Diseases3.55
Claire M. Jardine14
Estimated H-index: 14
(U of G: University of Guelph),
E. J. Parmley1
Estimated H-index: 1
(U of G: University of Guelph)
+ 2 AuthorsDavor Ojkic21
Estimated H-index: 21
(U of G: University of Guelph)
Published on Feb 11, 2018in Veterinarni Medicina0.64
M. Rahimi1
Estimated H-index: 1
Avian metapneumovirus causes an acute highly contagious upper respiratory tract infection pri - marily of turkeys and chickens. The disease can cause significant economic losses in turkey and chicken flocks, particularly when exacerbated by secondary pathogens. The purpose of this study was to determine the prevalence of avian metapneumovirus antibodies in broiler and broiler breeder flocks in Kermanshah province, west of Iran. All the flocks had not been vaccinated against avian metapneumovirus...
Published on Jan 1, 2018in Journal of Virological Methods1.75
Evelyne Lemaitre5
Estimated H-index: 5
(ANSES),
C. Allée6
Estimated H-index: 6
(ANSES)
+ 2 AuthorsPa. Brown1
Estimated H-index: 1
(ANSES)
Abstract Current molecular methods for the detection of avian and human metapneumovirus (AMPV, HMPV) are specifically targeted towards each virus species or individual subgroups of these. Here a broad range SYBR Green I real time RT-PCR was developed which amplified a highly conserved fragment of sequence in the N open reading frame. This method was sufficiently efficient and specific in detecting all MPVs. Its validation according to the NF U47-600 norm for the four AMPV subgroups estimated low...
Published on Oct 3, 2017in Autophagy11.06
Lei Hou4
Estimated H-index: 4
,
Li Wei8
Estimated H-index: 8
+ 4 AuthorsJue Liu12
Estimated H-index: 12
ABSTRACTAn increasing number of studies have demonstrated that macroautophagy/autophagy plays an important role in the infectious processes of diverse pathogens. However, it remains unknown whether autophagy is induced in avian metapneumovirus (aMPV)-infected host cells, and, if so, how this occurs. Here, we report that aMPV subgroup C (aMPV/C) induces autophagy in cultured cells. We demonstrated this relationship by detecting classical autophagic features, including the formation of autophagsom...
Published on Jun 1, 2017in Avian Diseases1.31
Giovanni Franzo10
Estimated H-index: 10
(UNIPD: University of Padua),
Claudia Maria Tucciarone5
Estimated H-index: 5
(UNIPD: University of Padua)
+ 4 AuthorsMattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
SUMMARY Avian metapneumovirus (aMPV) represents one of the most prevalent diseases of turkey, especially in combination with other pathogens, and its frequency is also increasing among chickens. Despite this evidence, epidemiologic data are poor and scattered, severely preventing control of the disease even in highly developed areas such as Europe. In the present study, the detection and characterization of an aMPV subtype B strain circulating in a vaccinated but symptomatic Romanian broiler flo...
Published on Dec 1, 2015in Molecular and Cellular Probes2.51
Sarita Paudel3
Estimated H-index: 3
(CNU: Chungnam National University),
Hyun-Jin Shin9
Estimated H-index: 9
(CNU: Chungnam National University)
Abstract To understand the molecular mechanisms of Avian metapneumovirus (aMPV) and the requirements involved in the infection and fusion, trypsin treatment was done in the different stages of virus; before infection, during entry and after virus infection followed by aMPV infection. The growth kinetics of aMPV was compared in time dependent manner. The effect of trypsin was found in the later stage of aMPV infection increasing the numbers of infected cells with the significant higher titer of i...
Published on Apr 1, 2015in Research in Veterinary Science1.75
Thunai Al-Shekaili1
Estimated H-index: 1
(University of Liverpool),
Matthew Baylis39
Estimated H-index: 39
(University of Liverpool),
Kannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Abstract Infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) are economically important viral pathogens infecting chickens globally. Identification of endemic IBV and aMPV strains promotes better control of both diseases and prevents production losses. Orophrayngeal swab samples were taken from 2317 birds within 243 different backyard flocks in Oman. Swabs from each flock were examined by RT-PCR using part-S1 and G gene primers for IBV and aMPV respectively. Thirty-nine chicken fl...
Published on Dec 1, 2014in Veterinary Research3.12
Shikai Sun2
Estimated H-index: 2
,
Feng Chen16
Estimated H-index: 16
(SCAU: South China Agricultural University)
+ 8 AuthorsJianping Qin8
Estimated H-index: 8
Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a ...
View next paperNucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup