Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius
Abstract
For reverse genetic approaches inactivation or selective modification of genes are required to elucidate their putative function. Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon which grows optimally at 76°C and pH 3. As many antibiotics do not withstand these conditions the development of a genetic system in this organism is dependent on auxotrophies. Therefore we constructed a pyrE deletion mutant of S. acidocaldarius wild type...
Paper Details
Title
Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius
Published Date
Jan 1, 2012
Journal
Volume
3
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