Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos

Reprogramming after somatic cell nuclear transfer had been thought to be dependent on the recipient cytoplasm being arrested at the metaphase stage, but here interphase two-cell mouse embryos are shown to support successful reprogramming and generation of embryonic stem cells or cloned mice. The reprogramming that occurs following somatic cell nuclear transfer (SCNT) during infertility treatment and other procedures is thought to be dependent on...