Effects of GABA and epinephrine on the settlement and metamorphosis of the larvae of four species of bivalve molluscs

Published on Mar 1, 2005in Journal of Experimental Marine Biology and Ecology2.365
· DOI :10.1016/j.jembe.2004.10.011
Montserrat Garcia-Lavandeira9
Estimated H-index: 9
(University of Santiago de Compostela),
Arturo Silva5
Estimated H-index: 5
(University of Santiago de Compostela)
+ 3 AuthorsM. Luz Pérez-Parallé9
Estimated H-index: 9
(University of Santiago de Compostela)
Competent larvae of different marine bivalve species were treated with GABA and epinephrine at different concentrations and times of exposure to test the ability of the drugs to induce settlement and metamorphosis. GABA induced both settlement and metamorphosis in the mussel Mytilus galloprovincialis, the clams Venerupis pullastra and Ruditapes philippinarum and the oyster Ostrea edulis. Maximum induction of settlement (N39%) was achieved after exposure of V. pullastra larvae to 10 4 M GABA; this concentration of GABA also induced the highest percentages of metamorphosis in the four species studied. Epinephrine was identified as an active inducer of settlement and metamorphosis in bivalve molluscs. Exposure to 10 5 M epinephrine induced significant levels of settlement in Mytilus, Venerupis and Ostrea. In contrast, epinephrine failed to induce settlement behaviour in Ruditapes. Maximum induction of metamorphosis was produced by 10 5 M epinephrine in mussels, clams and oysters; Ruditapes showed the highest percentage of metamorphosis (N78%). This is the first report in which the involvement of GABA in the settlement and metamorphosis of bivalve molluscan larvae is demonstrated. It was also recognised that epinephrine plays a role not only in inducing metamorphosis but also in initiating settlement. D 2004 Elsevier B.V. All rights reserved.
  • References (24)
  • Citations (80)
📖 Papers frequently viewed together
3 Authors (Bin Zhao, ..., Pei-Yuan Qian)
69 Citations
46 Citations
4 Authors (Daniel E. Morse, ..., Lloyd Jensen)
316 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Simon M. CraggH-Index: 16
38 Citations
#1Sergey Dobretsov (HKUST: Hong Kong University of Science and Technology)H-Index: 36
#2Pei-Yuan Qian (HKUST: Hong Kong University of Science and Technology)H-Index: 61
The blue mussel Mytilus edulis L. is an important aquaculture and fouling species in northern seas. Although the general role of chemical cues for settlement of larvae of the blue mussel has been proposed, few studies have focused on induction of settlement and metamorphosis by pharmacological agents. In this study, the induction of larval settlement of the blue mussel by pharmacological compounds was investigated through a series of laboratory experiments with an aim of identifying artificial c...
47 CitationsSource
#1Stanley C.K. Lau (HKUST: Hong Kong University of Science and Technology)H-Index: 23
#1Stanley C.K. Lau (HKUST: Hong Kong University of Science and Technology)H-Index: 21
Last. Pei-Yuan Qian (HKUST: Hong Kong University of Science and Technology)H-Index: 61
view all 3 authors...
Abstract Three bacterial isolates ( Micrococcus sp., Rhodovulum sp., and Vibrio sp.) from natural biofilms were investigated for their effects on cyprid settlement of Balanus amphitrite in laboratory bioassays. The inhibitive effect of these bacteria was clearly demonstrated by using a choice assay, in which cyprids settled preferentially on surfaces without bacterial pretreatment over those possessing a monospecies bacterial film. This result suggested that the inhibitive effect was mediated by...
53 CitationsSource
#1Mehdi S. Doroudi (JCU: James Cook University)H-Index: 8
#2Paul C. Southgate (JCU: James Cook University)H-Index: 32
Inducing more rapid or greater degree of settlement of blacklip pearl oyster (Pinctada margaritifera) larvae using chemical cues can significantly improve the commercial hatchery production of this valuable species. For this purpose, epinephrine, norepinephrine and γ-aminobutyric acid (GABA) were assessed at four concentrations (10−2, 10−3, 10−4 and 10−5 M), and in conjunction with plastic filaments for their ability to induce settlement of P. margaritifera larvae. In all concentrations tested, ...
46 CitationsSource
#1Daniel J. Jackson (UQ: University of Queensland)H-Index: 25
#1Daniel J. JacksonH-Index: 32
Last. Bernard M. Degnan (UQ: University of Queensland)H-Index: 59
view all 6 authors...
In the marine environment a wide range of invertebrates have a pelagobenthic lifecycle that includes planktonic larval and benthic adult phases. Transition between these morphologically and ecologically distinct phases typically occurs when the developmentally competent larva comes into contact with a species-specific environmental cue. This cue acts as a morphogenetic signal that induces the completion of the postlarval/juvenile/adult developmental program at metamorphosis. The development of c...
74 CitationsSource
Metamorphosis and settlement studies were conducted with 20 to 24-d-old laboratory-reared larvae of 4 closely related and genetically divergent sea urchins of the genus Echinometra (E. sp. nov. A, E. mathaei, E. sp. nov. C, and E. oblonga) to assess their preferences for various substrata. All the Echinometra spp. exhibited a similar high rate of metamorphosis in response to encrusting coralline red algae compared to mixed turfs of coralline algae with: regular brown, green, or mixed fleshy alga...
26 Citations
#1Esther M. Leise (UNCG: University of North Carolina at Greensboro)H-Index: 19
#2Michael G. HadfieldH-Index: 46
Larvae of the nudibranch mollusc Phestilla sibogae metamorphose in response to a small organic com-pound released into seawater by their adult prey, the scleractinian coral Porites compressa. The transformations that occur during metamorphosis, including loss of the ciliated velum (swimming organ), evacuation of the shell, and bodily elongation, are thought to be controlled by a combination of neuronal and neuroendocrine activities. Activation of peripheral chemosensory neurons by the metamorpho...
30 CitationsSource
#1Anthony Pires (Dickinson College)H-Index: 10
#2Roger P. CrollH-Index: 33
Last. Michael G. HadfieldH-Index: 46
view all 3 authors...
Larvae of the nudibranch Phestilla sibogae are induced to metamorphose by a factor from their adult prey, the coral Porites compressa. Levels of endogenous catecholamines increase 6 to 9 days after fertilization, when larvae become competent for metamorphosis. Six- to nine-day larvae, treated with the catecholamine precursor L-DOPA (0.01 mM for 0.5 h), were assayed for metamorphosis in response to coral inducer and for catecholamine content by high-performance liquid chromatography. L-DOPA treat...
61 CitationsSource
#1Michael G. Hadfield (UH: University of Hawaii)H-Index: 46
Last. Dmitri Y. BoudkoH-Index: 19
view all 3 authors...
On the basis of anatomy and larval behavior, the apical sensory organ (ASO) of gastropod veliger larvae has been implicated as the site of perception of cues for settlement and metamorphosis. Until now, there have been no experimental data to support this hypothesis. In this study, cells in the ASO of veliger larvae of the tropical nudibranch Phestilla sibogae were stained with the styryl vital dye DASPEI and then irradiated with a narrow excitatory light beam on a fluorescence microscope. When ...
123 CitationsSource
#1Patrick J. Bryan (HKUST: Hong Kong University of Science and Technology)H-Index: 16
#2Pei-Yuan Qian (HKUST: Hong Kong University of Science and Technology)H-Index: 61
The attachment and metamorphic responses of veliger larvae of the abalone, Haliotis diversicolor (Reeve) to potassium chloride (KCl), gamma-aminobutyric acid (GABA) and natural cues were determined. Attachment and metamorphosis of H. diversicolor were two distinctly different responses. Attachment was characterized by larval contact with a substratum followed by crawling with the larval foot firmly attached to the surface of the substratum. This behavior began approximately 48 h post-fertilizati...
74 CitationsSource
Cited By80
#1You-Ting Zhu (Shanghai Ocean University)H-Index: 1
#2Ya Zhang (Shanghai Ocean University)H-Index: 1
Last. Xiao Liang (Shanghai Ocean University)H-Index: 3
view all 8 authors...
Marine invertebrates undergo distinct life cycles and development processes, and some undergo a metamorphic transition from pelagic larvae to benthic juvenile. Previous studies have shown that the second messenger nitric oxide (NO) plays important roles in regulating larval metamorphosis in marine invertebrates. However, this regulatory function is not conserved. Herein, we investigated the regulatory role of NO in larval metamorphosis in Hard-shelled mussel (Mytilus coruscus). Pharmacological e...
#1Jodi T. Thomas (JCU: James Cook University)H-Index: 1
#2Philip L. Munday (JCU: James Cook University)H-Index: 68
Last. Sue-Ann Watson (JCU: James Cook University)H-Index: 24
view all 3 authors...
Elevated carbon dioxide (CO2) levels can alter ecologically important behaviours in a range of marine invertebrate taxa; however, a clear mechanistic understanding of these behavioural changes is lacking. The majority of mechanistic research on the behavioural effects of elevated CO2 has been done in fish, focusing on disrupted functioning of the GABAA receptor (a ligand-gated ion channel, LGIC). Yet, elevated CO2 could induce behavioural alterations through a range of mechanisms that disturb di...
Aquaculture and capture production of clam species have great economic value in Europe. The production is dominated by the introduced species, Manila clam, Ruditapes philippinarum, which has displaced some of the native species of clams. In Europe, landings of autochthonous clams have decreased in the recent years due to overfishing, failure in recruitment, abiotic stress and diseases. Due to aforementioned factors, seed production in hatcheries has become essential to ensure the sustainability ...
#1Akiko Hozumi (University of Tsukuba)H-Index: 12
#2Shohei Matsunobu (University of Tsukuba)H-Index: 1
Last. Mayuko Hamada (Okayama University)H-Index: 1
view all 14 authors...
Summary Metamorphosis, a widespread life history strategy in metazoans, allows dispersal and use of different ecological niches through a dramatic body change from a larval stage [ 1 , 2 ]. Despite its conservation and importance, the molecular mechanisms underlying its initiation and progression have been characterized in only a few animal models. In this study, through pharmacological and gene functional analyses, we identified neurotransmitters responsible for metamorphosis of the ascidian Ci...
#1Li-Hua Peng (Shanghai Ocean University)H-Index: 1
#2Xiao Liang (Shanghai Ocean University)H-Index: 3
Last. Jin-Long Yang (Shanghai Ocean University)H-Index: 2
view all 5 authors...
As a stage of life cycle, larval settlement and metamorphosis are critical processes for persistence of many marine invertebrate populations. Bacterial biofilms (BFs) could induce larval settlement and metamorphosis. Pseudoalteromonas, a widely distributed genus of marine bacteria, showed inductive effects on several invertebrates. However, how Pseudoalteromonas BFs induce settlement and metamorphosis of Mytilus coruscus remains unclear. Pseudoalteromonas marina BFs with the highest inducing act...
#1Jean Rose H. Maquirang (College of Fisheries)H-Index: 1
#2Fiona L. Pedroso (University of the Philippines Visayas)H-Index: 1
Last. Carlos C. Baylon (University of the Philippines Visayas)H-Index: 2
view all 8 authors...
Nutrition plays a critical role in the growth and development of green mussel Perna viridis larvae. In this study, three species of tropical microalgae were investigated to determine their efficacy as feed source for the pediveliger stage. Larvae (2 larvae ml−1) were given Isochrysis galbana (Iso), Chaetoceros calcitrans (Cc), and Tetraselmis tetrahele (Tt) at 100,000 cells ml−1. No statistically significant difference in survival was found among treatments. The Cc diet promoted maximum growth i...
#1Xiao Liang (Ministry of Science and Technology)H-Index: 3
#2Ke Chen (Ministry of Science and Technology)H-Index: 1
Last. Jin-Long Yang (Ministry of Science and Technology)H-Index: 2
view all 7 authors...
AbstractMetamorphosis is crucial in the life-cycle transition between the larval and juvenile stages of marine invertebrates. Although a number of agonists and antagonists of the adrenergic recepto...
1 CitationsSource
#1Susanne Vogeler (University of Gothenburg)H-Index: 4
#2Gary H. Wikfors (NMFS: National Marine Fisheries Service)H-Index: 31
Last. Alyssa Joyce (University of Gothenburg)H-Index: 5
view all 6 authors...
Abstract Over the last three decades, a variety of chemical inducers or disruptors for settlement and metamorphosis have been identified for species of clams, oysters, scallops, and mussels. Identifying inducers is important in shellfish aquaculture for increasing metamorphosis rates, and hence, spat (seed) production; however, it also has wider applications, such as in the development of marine anti-fouling agents. Previously, we have suggested the potential importance of an N-Methyl- d -aspart...
1 CitationsSource
#1Shaokun Lu (NBU: Ningbo University)H-Index: 1
#2Ronghua Li (NBU: Ningbo University)H-Index: 7
Last. Chunlin Wang (NBU: Ningbo University)H-Index: 15
view all 8 authors...
#1S. Mercurio (University of Milan)H-Index: 4
#2Giacomo Gattoni (University of Milan)
Last. Roberta Pennati (University of Milan)H-Index: 1
view all 5 authors...
: Neural development of echinoderms has always been difficult to interpret, as larval neurons degenerate at metamorphosis and a tripartite nervous system differentiates in the adult. Despite their key phylogenetic position as basal echinoderms, crinoids have been scarcely studied in developmental research. However, since they are the only extant echinoderms retaining the ancestral body plan of the group, crinoids are extremely valuable models to clarify neural evolution in deuterostomes. Antedon...