Deep Proteomics of the Xenopus laevis Egg using an mRNA-Derived Reference Database

BackgroundMass spectrometry-based proteomics enables the global identification and quantification of proteins and their posttranslational modifications in complex biological samples. However, proteomic analysis requires a complete and accurate reference set of proteins and is therefore largely restricted to model organisms with sequenced genomes.ResultsHere, we demonstrate the feasibility of deep genome-free proteomics by using a reference...