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PCR cloning of the full-length cDNA for the seed protein canavalin from the jack bean plant,Canavalis ensiformis

Published on Jan 1, 1992in Plant Molecular Biology 3.93
· DOI :10.1007/BF00018469
Joseph D. Ng3
Estimated H-index: 3
(UCR: University of California, Riverside),
Timothy Stinchcombe1
Estimated H-index: 1
(UCR: University of California, Riverside)
+ 2 AuthorsAlexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
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  • References (5)
  • Citations (3)
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References5
Newest
Published on Nov 1, 1989in Molecular Biology and Evolution 14.80
Peter E. M. Gibbs23
Estimated H-index: 23
(UCR: University of California, Riverside),
Kara B. Strongin1
Estimated H-index: 1
(UCR: University of California, Riverside),
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
We examined the primary sequence of canavalin, the major storage protein of jack beans, and found that an ancient sequence duplication accounts for 80% of the amino acid residues. Evidence for such a duplication was also found in the orthologous proteins phaseolin and pea vicilin. This sequence duplication presumably accounts for a structural duplication in the canavalin monomer observed by crystallographic analysis. One copy of this repeat was found in a second storage-protein family, the legum...
Published on Jan 1, 1988in FEBS Journal 4.74
Daisuke Yamauchi15
Estimated H-index: 15
(Tokyo Metropolitan University),
Kenzo Nakamura46
Estimated H-index: 46
(Nagoya University)
+ 1 AuthorsTakao Minamikawa24
Estimated H-index: 24
(Tokyo Metropolitan University)
By a method of Escherichia coli expression-vector-primed cDNA synthesis, a cDNA expression library was constructed from total poly(A)-rich RNA that was prepared from immature embryos of Canavalia gladiata. Essentially full-length cDNA clones for two seed proteins, canavalin and concanavalin A, were selected from the library by immunological screening of the colonies and in vitro RNA synthesis and translation. The complete amino acid sequence of canavalin was determined from the nucleotide sequen...
Published on Jan 1, 1987in Methods in Enzymology 1.86
Marc S. Krug3
Estimated H-index: 3
,
Shelby L. Berger20
Estimated H-index: 20
The quality of a cDNA library depends on the integrity of the messenger RNA and the fidelity with which it can be reverse transcribed. RNA cannot be cloned directly; in a reaction catalyzed by reverse transcriptase, the RNA, together with a suitable primer and a supply of deoxyribonucleoside triphosphates (dNTPs), must be converted to a double-stranded molecule. The product contains a complementary strand (first, antisense, or minus-strand cDNA) that is hybridized to what remains of the original...
Kary B. Mullis20
Estimated H-index: 20
,
Fred A. Faloona5
Estimated H-index: 5
+ 3 AuthorsHenry A. Erlich94
Estimated H-index: 94
Method for the manufacture of expanded confection products in which the confection material before expansion is made into the form of an aggregate of granules that are fused or bonded together to form a slug or wafer. This slug or wafer is heated to a plastic state and subsequently cooled. After cooling, the product is placed in contact with a mass of cooked dough, heated to soften the same followed by expanding with the aid of a partial vacuum.
F. Sanger43
Estimated H-index: 43
,
S Nicklen2
Estimated H-index: 2
,
A.R. Coulson23
Estimated H-index: 23
Abstract A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the m...
Cited By3
Newest
Published on Dec 1, 2016in Bioscience, Biotechnology, and Biochemistry 1.30
Kaho Nishizawa2
Estimated H-index: 2
(Mukogawa Women's University),
Yasuhiro Arii4
Estimated H-index: 4
(Mukogawa Women's University)
Canavalin is a vicilin-class (7S) storage protein found in sword bean (Canavalia gladiata). Our previous report indicated that canavalin is precipitated by the addition of 20 mM MgCl2 to crude sword bean extract. Here, we examined the solubility changes induced by the addition of Mg2+ and Ca2+ at various concentrations. Canavalin tended to be insolubilized at relatively low concentrations of MgCl2 ( 20 mM). In addition, canavalin was slightly insolubilized in the presence of NaCl. Overall, the r...
Published on Aug 2, 2016in Bioscience, Biotechnology, and Biochemistry 1.30
Kaho Nishizawa2
Estimated H-index: 2
(Mukogawa Women's University),
Tetsuya Masuda17
Estimated H-index: 17
(Kyoto University)
+ 3 AuthorsYasuhiro Arii4
Estimated H-index: 4
(Mukogawa Women's University)
Sword bean (Canavalia gladiata) seeds are a traditional food in Asian countries. In this study, we aimed to determine the optimal methods for the precipitation of sword bean proteins useful for the food development. The soaking time for sword beans was determined by comparing it with that for soybeans. Sword bean proteins were extracted from dried seeds in distilled water using novel methods. We found that most proteins could be precipitated by heating the extract at more than 90 °C. Interesting...
Published on Jan 1, 1998in Cells Tissues Organs 1.33
H. Rüdiger1
Estimated H-index: 1
Plant lectins are easily available, fairly stable and suitable for many kinds of chemical modification. Thus, they have become important tools in glycosciences. In the present review, it is attempted to throw light upon aspects of lectinology that deal with their natural occurrence, biosynthesis, structure, binding specificities and hypotheses about their biological functions.
Published on Oct 1, 1992in Protein Science 2.42
John Day15
Estimated H-index: 15
(UCR: University of California, Riverside),
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Macromolecular crystal growth experiments, using satellite tobacco mosaic virus (STMV) and canavalin from jack beans as samples, were conducted on a US Space Shuttle mission designated International Microgravity Laboratory — 1 (IML-1), flown January 22–29, 1992. Parallel experiments using identical samples were carried out in both a vapor diffusion-based device (PCG) and a liquid-liquid diffusion-based instrument (CRYOSTAT). The experiments in each device were run at 20–22 °C and at colder tempe...