Multidimensional Protein Identification Technology-Selected Reaction Monitoring Improving Detection and Quantification for Protein Biomarker Studies

The targeted analysis of proteins in complex biological samples is best achieved using selected reaction monitoring (SRM). To maximize the sensitivity of this approach, sample fractionation or enrichment is still required, particularly to detect less abundant proteins in clinically relevant biofluids. Here, we report the development of multidimensional protein identification technology (MudPIT)-SRM, taking advantage of the robust online strong...