MiR-143 and rat embryo implantation

To study the role of miR-143 during embryo implantation in rat.MiR-143 expression in rat early pregnancy was detected by Northern blot. The relation between miR-143 and Lifr predicted and confirmed by bioinformatics method, dual-luciferase activity assay, Western blot and immunohistochemistry. The role of miR-143 was detected by MTS, Edu and ranswell chamber assays.The expression level of miR-143 on gestation day 5-8 (g.d. 5-8) was higher than...