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Electrochemical Characterization of Globotriose-Containing Self-Assembled Monolayers on Nanoporous Gold and their Binding of Soybean Agglutinin

Published on May 1, 2013in Carbohydrate Research1.87
· DOI :10.1016/j.carres.2012.09.021
Binod Pandey7
Estimated H-index: 7
(UMSL: University of Missouri–St. Louis),
Yih Horng Tan11
Estimated H-index: 11
(UMSL: University of Missouri–St. Louis)
+ 3 AuthorsKeith J. Stine21
Estimated H-index: 21
(UMSL: University of Missouri–St. Louis)
Abstract
Abstract Self-assembled monolayers (SAMs) of α- d -Gal-(1→4)-β- d -Gal-(1→4)-β- d -Glc-mercaptooctane (globotriose, Gb3-C 8 -SH) were prepared both as single-component SAMs and as mixed SAMs with either octanethiol (OCT) or 8-mercapto-3,6-dioxaoctanol (HO-PEG 2 -SH), on flat gold and on nanoporous gold (NPG) electrodes. The binding of soybean agglutinin (SBA) to the globotriose (Gb3) unit in the SAMs was then studied using electrochemical impedance spectroscopy (EIS), which is a label free method found to be quite sensitive to SAM composition and to the differences in SAM structure on NPG versus on flat Au. The affinity of SBA to the mixed SAM of HO-PEG 2 -SH and Gb3-C 8 -SH on NPG is found to be greater on NPG than on flat gold, and indicates a potential advantage for NPG as a substrate. The SAMs of HO-PEG 2 -SH were found to resist protein adsorption on either NPG or flat gold. The non-specific adsorption of SBA to OCT SAMs on flat Au was observed in EIS by the increase in charge transfer resistance; whereas, the increase seen on the NPG surface was smaller, and suggests that EIS measurements on NPG are less affected by non-specific protein adsorption. Atomic force microscopy (AFM) images of the SBA binding to mixed SAM of HO-PEG 2 -SH and Gb3-C 8 -SH on NPG showed a greater number of proteins on top of the OCT containing SAMs.
  • References (75)
  • Citations (9)
References75
Newest
#1Binod Pandey (UMSL: University of Missouri–St. Louis)H-Index: 7
#2Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 21
view all 5 authors...
#1Fengna Xi (CAS: Chinese Academy of Sciences)H-Index: 1
#2Jingqing Gao (CAS: Chinese Academy of Sciences)H-Index: 1
Last.Zhenxin Wang (CAS: Chinese Academy of Sciences)H-Index: 35
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#1Xing-You Lang (Tohoku University)H-Index: 28
#2Lihua Qian (Tohoku University)H-Index: 22
Last.Mingwei Chen (Tohoku University)H-Index: 86
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#1Dawei Wang (NU: Northwestern University)H-Index: 22
#2Rikkert J. Nap (NU: Northwestern University)H-Index: 15
Last.Igal Szleifer (NU: Northwestern University)H-Index: 51
view all 7 authors...
#1Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
#2John R. Schallom (UMSL: University of Missouri–St. Louis)H-Index: 1
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 21
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Cited By9
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#1Jay K. Bhattarai (UMSL: University of Missouri–St. Louis)H-Index: 6
#2Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 21
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#1Preeti Madhukar Chaudhary (IISc: Indian Institute of Science)H-Index: 3
#2Madhuri Gade (IISc: Indian Institute of Science)H-Index: 3
Last.Raghavendra Kikkeri (IISc: Indian Institute of Science)H-Index: 8
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#1Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 21
#1Andras Hushegyi (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 3
#2Ludmila Klukova (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 5
Last.Jan Tkac (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 32
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#1Aline Debrassi (WUR: Wageningen University and Research Centre)H-Index: 4
#2W.M. de Vos (UH: University of Helsinki)H-Index: 134
Last.Tom Wennekes (WUR: Wageningen University and Research Centre)H-Index: 14
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#1Leila Kashefi-Kheyrabadi (UI: University of Isfahan)H-Index: 5
#2Abolhassan Noori (TMU: Tarbiat Modares University)H-Index: 14
Last.Masoud A. Mehrgardi (UI: University of Isfahan)H-Index: 15
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#1Andras Hushegyi (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 3
#2Jan Tkac (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 32
#1Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
#2Kohki Fujikawa (UMSL: University of Missouri–St. Louis)H-Index: 10
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 21
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