Modifications of the DNA secondary structure upon platinum binding: a proposed model

A number of physico-chemical techniques were used to study the interaction between a series of platinum compounds and DNA. Spectrofluorometry, ultraviolet spectroscopy (UV), circular dichroism (CD), melting curves, kinetics and electron microscopy (EM) were found powerful in the differentiation of the DNA secondary structure modifications upon platinum binding. Three types of alterations are clearly shown and can be correlated with the mode of...