Crystal structure of the human Fab fragment Kol and its comparison with the intact Kol molecule

The crystal structure of the Fab‡ fragment of the IgG protein Kol was analysed and an electron density map calculated at 3 Å resolution based on phases obtained from multiple isomorphous replacement. An atomic model was constructed but the lack of amino acid sequence data causes some uncertainty, in particular concerning the amino acid side-chains. Several cycles of constrained crystallographic refinement (Deisenhofer & Steigemann, 1975)...