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Diagnostic of precipitant for biomacromolecule crystallization by quasi-elastic light-scattering

Published on May 1, 1990in Journal of Molecular Biology5.07
· DOI :10.1016/S0022-2836(05)80130-5
Vincent Mikol7
Estimated H-index: 7
(CNRS: Centre national de la recherche scientifique),
Ernest Hirsch6
Estimated H-index: 6
,
Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique)
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Abstract
The translational diffusion coefficient D 25,w of hen egg-white lysozyme and concanavalin A from the jack bean is measured in various precipitating agent solutions as a function of salt and protein concentration using quasi-elastic light-scattering. With some precipitants, in undersaturated protein solutions, a protein or salt concentration dependence of the diffision coefficient of the scatterers is observed. It can be correlated with the inability of the protein to crystallize in this precipitant once the solution is supersaturated. These variations of D 25,w are interpreted in terms of non-specific interactions and/or aggregation that prevent the protein from making appropriate contacts to form a crystal. With other precipitants known to lead to crystallization, no significant variation of the diffusion coefficient with increasing concentration was observed, indicating that under such conditions up to saturation the proteins remain essentially monodisperse. Application of this technique to find crystallization conditions of other proteins is discussed.
  • References (25)
  • Citations (103)
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References25
Newest
#1Webe Kadima (UCR: University of California, Riverside)H-Index: 4
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
Last.Frances Jurnak (UCR: University of California, Riverside)H-Index: 25
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#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Ernest HirschH-Index: 6
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 53
view all 3 authors...
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 53
#2Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#1Charles W. Carter (UNC: University of North Carolina at Chapel Hill)H-Index: 7
#2Eric T. Baldwin (UNC: University of North Carolina at Chapel Hill)H-Index: 1
Last.Lloyd Frick (UNC: University of North Carolina at Chapel Hill)H-Index: 7
view all 3 authors...
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 53
#2Anne-Catherine Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last.Dino Moras (CNRS: Centre national de la recherche scientifique)H-Index: 79
view all 6 authors...
Cited By103
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#1Stefania Perticaroli (UT: University of Tennessee)H-Index: 13
#2Jonathan D. Nickels (UT: University of Tennessee)H-Index: 16
Last.Alexei P. Sokolov (UT: University of Tennessee)H-Index: 48
view all 4 authors...
#1Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
#2Kohki Fujikawa (UMSL: University of Missouri–St. Louis)H-Index: 11
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
view all 7 authors...
#1Bernard Lorber (UDS: University of Strasbourg)H-Index: 28
#2Frédéric Fischer (UDS: University of Strasbourg)H-Index: 5
Last.Daniel Kern (UDS: University of Strasbourg)H-Index: 33
view all 5 authors...
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