Genotyping with CRISPR-Cas-derived RNA-guided endonucleases

Volume: 5, Issue: 1, Pages: 3157
Published: Jan 20, 2014
Abstract
Restriction fragment length polymorphism (RFLP) analysis is one of the oldest, most convenient and least expensive methods of genotyping, but is limited by the availability of restriction endonuclease sites. Here we present a novel method of employing CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) in RFLP analysis. We prepare RGENs by complexing recombinant Cas9 protein derived from Streptococcus pyogenes with in vitro transcribed...
Paper Details
Title
Genotyping with CRISPR-Cas-derived RNA-guided endonucleases
Published Date
Jan 20, 2014
Volume
5
Issue
1
Pages
3157
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