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Genotyping with CRISPR-Cas-derived RNA-guided endonucleases

Published on May 1, 2014in Nature Communications 12.35
· DOI :10.1038/ncomms4157
Jong Min Kim21
Estimated H-index: 21
,
Daesik Kim12
Estimated H-index: 12
+ 1 AuthorsJin-Soo Kim52
Estimated H-index: 52
Abstract
Cas9 RNA-guided engineered nucleases (RGENs) induce site-specific DNA cleavages in cultured cells and organisms and are used widely as genome-editing tools. Here, the authors develop an RGEN-based technology to genotype both RGEN-induced mutations and cancer-associated mutations in human cell lines.
  • References (33)
  • Citations (60)
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References33
Newest
Published on Jan 1, 2014in Genome Research 10.10
Young Hoon Sung10
Estimated H-index: 10
(Yonsei University),
Jong Min Kim1
Estimated H-index: 1
(Seoul National University)
+ 9 AuthorsCheol-Hee Kim36
Estimated H-index: 36
(Chungnam National University)
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the m...
173 Citations Source Cite
Published on Jan 1, 2014in Genome Research 10.10
Seung Woo Cho25
Estimated H-index: 25
(Seoul National University),
Sojung Kim10
Estimated H-index: 10
(Seoul National University)
+ 4 AuthorsJin-Soo Kim52
Estimated H-index: 52
(Seoul National University)
RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enzymes cleave chromosomal DNA, whose sequence is complementary, to guide RNA in a targeted manner, producing site-specific DNA double-strand breaks (DSBs), the repair of which gives rise to targeted gen...
675 Citations Source Cite
Published on Nov 1, 2013in Genetics 4.08
Seung Woo Cho25
Estimated H-index: 25
(Seoul National University),
Jihyun Lee3
Estimated H-index: 3
(Seoul National University)
+ 2 AuthorsJunho Lee29
Estimated H-index: 29
(Seoul National University)
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method.
164 Citations Source Cite
Published on Sep 1, 2013in Nature Biotechnology 35.72
Patrick Hsu15
Estimated H-index: 15
,
Douglas Scott96
Estimated H-index: 96
+ 11 AuthorsOphir Shalem18
Estimated H-index: 18
Analyses of the determinants of the specificity of Cas9 nuclease provide rules for selecting optimal target sites.
2,006 Citations Source Cite
Published on Aug 1, 2013in Genetics 4.08
Scott J. Gratz9
Estimated H-index: 9
(University of Wisconsin-Madison),
Alexander M. Cummings3
Estimated H-index: 3
(University of Wisconsin-Madison)
+ 5 AuthorsKate M. O’Connor-Giles6
Estimated H-index: 6
(University of Wisconsin-Madison)
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
539 Citations Source Cite
Published on Aug 1, 2013in Nature Biotechnology 35.72
Vladimir Nekrasov14
Estimated H-index: 14
,
Brian J. Staskawicz75
Estimated H-index: 75
+ 2 AuthorsSophien Kamoun82
Estimated H-index: 82
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
449 Citations Source Cite
Published on Aug 1, 2013in Nature Biotechnology 35.72
Qiwei Shan7
Estimated H-index: 7
,
Yanpeng Wang12
Estimated H-index: 12
(Chinese Academy of Sciences)
+ 8 AuthorsJin-Long Qiu17
Estimated H-index: 17
1. Jinek, M. et al. Science 337, 816–821 (2012). 2. Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Nat. Biotechnol. 31, 230–232 (2013). 3. Cong, L. et al. Science 339, 819–823 (2013). 4. Mali, P. et al. Science 339, 823–826 (2013). 5. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013). 6. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013). 7. Wang, H. et al. Cell 153, 910–918 (2013). 8. Geurts, A.M. et al. Science 325, 433 (2009). 9. Tong, C., Li, P.,...
737 Citations Source Cite
Published on Aug 1, 2013in Nature Biotechnology 35.72
Jian-Feng Li15
Estimated H-index: 15
,
Julie E. Norville9
Estimated H-index: 9
+ 5 AuthorsJen Sheen71
Estimated H-index: 71
Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9
612 Citations Source Cite
Published on May 1, 2013in Cell 31.40
Haoyi Wang24
Estimated H-index: 24
(Massachusetts Institute of Technology),
Hui Yang16
Estimated H-index: 16
(Massachusetts Institute of Technology)
+ 4 AuthorsRudolf Jaenisch173
Estimated H-index: 173
(Massachusetts Institute of Technology)
SUMMARY Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient gene-targeting technology with the potential for multiplexed genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty - 8 alleles) in mouse embryonic stem (ES) cell...
2,009 Citations Source Cite
Published on May 1, 2013in Cell Research 15.39
Bin Shen14
Estimated H-index: 14
,
Jun Zhang3
Estimated H-index: 3
+ 6 AuthorsXingxu Huang14
Estimated H-index: 14
374 Citations Source Cite
Cited By60
Newest
Published on May 17, 2019in Scientific Reports 4.12
Alex K. Datsomor (Norwegian University of Science and Technology), Nikola Zic (Norwegian University of Science and Technology)+ 7 AuthorsPer Winge24
Estimated H-index: 24
(Norwegian University of Science and Technology)
Atlantic salmon can synthesize polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (20:5n-3), arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) via activities of very long chain fatty acyl elongases (Elovls) and fatty acyl desaturases (Fads), albeit to a limited degree. Understanding molecular mechanisms of PUFA biosynthesis and regulation is a pre-requisite for sustainable use of vegetable oils in aquafeeds as current sources of fish oils are unable to meet increasing...
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Published on Mar 20, 2019in Scientific Reports 4.12
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University),
Sangsu Bae13
Estimated H-index: 13
(Hanyang University)
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
1 Citations Source Cite
Published on May 14, 2019in bioRxiv
Renyu Li (University of Virginia), Charles Vavrik (University of Virginia), Cristian H. Danna (University of Virginia)
CRISPR-Cas9 has become the preferred gene editing technology to obtain loss-of-function mutants in plants, and hence a valuable tool to study gene function. This is mainly due to the easy reprograming of Cas9 specificity using customizable small non-coding RNAs, and to the ability to target several independent genes simultaneously. Despite these advances, the identification of CRISPR-edited plants remains time and resource consuming. Here, based on the premise that one editing event in one locus...
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Published on Apr 1, 2019in Plant Cell Reports 2.99
Sukumar Biswas1
Estimated H-index: 1
(Shanghai Jiao Tong University),
Rong Li4
Estimated H-index: 4
(Shanghai Jiao Tong University)
+ 3 AuthorsJianxin Shi19
Estimated H-index: 19
(Shanghai Jiao Tong University)
Key message Two methods, PCR and amplicon labeling based, were developed and successfully applied to reliably detect CRISPR/Cas9 induced indels in rice.
1 Citations Source Cite
Published on Feb 5, 2019in Current protocols in human genetics
Arivazhagan Rajendran19
Estimated H-index: 19
(Kyoto University),
Narumi Shigi7
Estimated H-index: 7
(University of Tokyo)
+ 1 AuthorsMakoto Komiyama49
Estimated H-index: 49
(Ocean University of China)
1 Citations Source Cite
Published on Jan 1, 2019in Progress in Retinal and Eye Research 11.65
Jia Hui Lee3
Estimated H-index: 3
,
Jiang-Hui Wang5
Estimated H-index: 5
(University of Melbourne)
+ 4 AuthorsGuei-Sheung Liu18
Estimated H-index: 18
Abstract Many clinical trials using gene therapy have shown significant therapeutic benefits and exceptional safety records. Increasing evidence is verifying the long sought-after promise that gene therapy will genetically ‘cure’ some severely disabling diseases. In particular, the first gene therapy bioproduct for RPE65 -associated Leber's congenital amaurosis, which was approved by the US Food and Drug Administration in 2017, has provided tremendous encouragement to the field of gene therapy. ...
5 Citations Source Cite
Published on Dec 1, 2018in Molecular therapy. Nucleic acids 5.66
Emily Xia2
Estimated H-index: 2
(University of Toronto),
Rongqi Duan4
Estimated H-index: 4
+ 3 AuthorsJim Hu30
Estimated H-index: 30
(University of Toronto)
The CRISPR-Cas9 system is attractive for gene therapy, as it allows for permanent genetic correction. However, as a new technology, Cas9 gene editing in clinical applications faces major challenges, such as safe delivery and gene targeting efficiency. Cas9 is a foreign protein to recipient cells; thus, its expression may prompt the immune system to eliminate gene-edited cells. To overcome these challenges, we have engineered a novel delivery system based on the helper-dependent adenoviral (HD-Ad...
2 Citations Source Cite
Published on Dec 1, 2018in Plant Biotechnology Journal 6.30
Zhen Liang30
Estimated H-index: 30
(Chinese Academy of Sciences),
Kunling Chen14
Estimated H-index: 14
(Chinese Academy of Sciences)
+ 2 AuthorsCaixia Gao25
Estimated H-index: 25
(Chinese Academy of Sciences)
5 Citations Source Cite
Tianyuan Su1
Estimated H-index: 1
(Shandong University),
Haiying Jin3
Estimated H-index: 3
(Shandong University)
+ 4 AuthorsQingsheng Qi1
Estimated H-index: 1
(Chinese Academy of Sciences)
1 Citations Source Cite
Published on Dec 1, 2018in Scientific Reports 4.12
Diwakar Santhakumar2
Estimated H-index: 2
(Lancaster University),
Mohammed A. Rohaim4
Estimated H-index: 4
(Lancaster University)
+ 7 AuthorsMuhammad Munir17
Estimated H-index: 17
(Lancaster University)
The intracellular actions of interferon (IFN)-regulated proteins, including IFN-induced proteins with tetratricopeptide repeats (IFITs), attribute a major component of the protective antiviral host defense. Here we applied genomics approaches to annotate the chicken IFIT locus and currently identified a single IFIT (chIFIT5) gene. The profound transcriptional level of this effector of innate immunity was mapped within its unique cis-acting elements. This highly virus- and IFN-responsive chIFIT5 ...
4 Citations Source Cite