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Crystallography of hemerythrin

Published on Sep 1, 1974in Journal of Molecular Biology5.07
· DOI :10.1016/0022-2836(74)90298-8
A. C. T. North2
Estimated H-index: 2
,
Gerald Stubbs24
Estimated H-index: 24
Cite
Abstract
Abstract X-ray crystallographic studies of hemerythrin from Golfingia gouldii show that the molecules pack in a tetragonal cell with two sets of molecules in an apparently face-centred array seen in projection along the 4-fold axis, but displaced relatively to each other by approximately c 10 in the axial direction. Both sets of molecules lie on 4-fold rotation axes, so that the subunits of each octameric molecule are related in pairs by a molecular 4-fold rotation axis. The two subunits of each pair are probably related by non-crystallographic 2-fold rotation axes perpendicular to the 4-fold axes and lying at 10 ° and 55 ° to the a and b axes. At low resolution the subunits are apparently arranged approximately in the form of a square prism. Along each 4-fold crystallographic axis there are two hemerythrin molecules, nearly equidistant and having similar but not identical orientations.
  • References (7)
  • Citations (7)
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References7
Newest
Published on Dec 1, 1969in Biochemistry2.95
Neal R. Langerman2
Estimated H-index: 2
,
Irving M. Klotz53
Estimated H-index: 53
Published on Nov 1, 1969in Journal of Molecular Biology5.07
David L. D. Caspar1
Estimated H-index: 1
(National Foundation for Cancer Research),
Kenneth C. Holmes41
Estimated H-index: 41
(MPG: Max Planck Society)
Abstract X-ray diffraction photographs from dahlemense strain of tobacco mosaic virus show that it is very similar to the common strain, but additional meridional and near-meridional diffraction maxima appear on layer lines halfway between those given by the common strain. These are interpreted as arising from a regular perturbation of the arrangement of the outer part of the protein subunit. A theoretical discussion of the effects of a periodic perturbation on the diffraction pattern is given w...
Published on Jan 1, 1969in Archives of Biochemistry and Biophysics3.56
K. Garbett4
Estimated H-index: 4
(NU: Northwestern University),
Dennis W. Darnall8
Estimated H-index: 8
(NU: Northwestern University)
+ 1 AuthorsR.J.P. Williams1
Estimated H-index: 1
(NU: Northwestern University)
Abstract The absorption and circular dichroic spectra of hemerythrin in its deoxy, oxy, and several met states have been interpreted by comparison with those of several model systems and correlated with Mossbauer and magnetic data. The properties of the methemerythrin complexes are consistent with the presence of a dimeric pair of high spin iron (III) atoms antiferromagnetically coupled via an oxo bridge. It is proposed that oxyhemerythrin also has the same structure as methemerythrin, the oxyge...
Published on Apr 1, 1968in Journal of Molecular Biology5.07
B.W. Matthews10
Estimated H-index: 10
(LMB: Laboratory of Molecular Biology)
Abstract An analysis has been made, from the data which are currently available, of the solvent content of 116 different crystal forms of globular proteins. The fraction of the crystal volume occupied by solvent is most commonly near 43 %, but has been observed to have values from about 27 to 65%. In many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the p...
Published on May 1, 1963in Biochemistry2.95
Irving M. Klotz53
Estimated H-index: 53
,
Steven Keresztes-Nagy7
Estimated H-index: 7
Cited By7
Newest
Published on Aug 1, 1987in Journal of Molecular Biology5.07
R.E. Fenna12
Estimated H-index: 12
(UM: University of Miami)
Three crystal forms of canine myeloperoxidase are described. An orthorhombic form in space group P212121 has unit cell dimensions: a = 108.3 A (1 A = 0.1 nm) b = 205.9 A and c = 139.9 A. A trigonal form in space group P3121 or P3221 has unit cell dimensions: a = b = 138.9 A and c = 145.2 A. A monoclinic form in space group C2 has unit cell dimensions: a = 117.2 A, b = 96.9 A, c = 131.4 A and β = 116.3 °. Unusual features in the diffraction patterns of the monoclinic form place restrictions on th...
Published on Apr 23, 1976in Science41.04
Irving M. Klotz53
Estimated H-index: 53
,
Gerald L. Klippenstein3
Estimated H-index: 3
,
Wa Hendrickson1
Estimated H-index: 1
Published on Jan 1, 1976in Journal of Molecular Biology5.07
Ronald E. Stenkamp46
Estimated H-index: 46
(UW: University of Washington),
Larry C. Sieker39
Estimated H-index: 39
(UW: University of Washington)
+ 1 AuthorsJoann S. Loehr11
Estimated H-index: 11
(PSU: Portland State University)
Methemerythrin from Themiste dyscritum crystallizes in the tetragonal system with a=b =86·58(4) A, c =80·84(4) A,A, space group P 4 and two octameric molecules of weight ≈108,000 daltons per unit cell. Since the molecules were found to lie on the 4-fold axes, the asymmetric unit is composed of two subunits from one of the molecules in the unit cell and two from the other one. The electron density map at 5 A resolution was based on phases calculated from a single mercury iodide derivative. The mo...
Published on Jan 1, 1976
Charlotte P. Mangum1
Estimated H-index: 1
Published on Jan 1, 1976
Charlotte P. Mangum29
Estimated H-index: 29
(W&M: College of William & Mary)
Published on Oct 1, 1975in Nature43.07
Keith B. Ward12
Estimated H-index: 12
(NRL: United States Naval Research Laboratory),
Wayne A. Hendrickson83
Estimated H-index: 83
(NRL: United States Naval Research Laboratory),
Gerald L. Klippenstein5
Estimated H-index: 5
(UNH: University of New Hampshire)
THE oligomeric protein haemerythrin is an oxygen-transport pigment found in erythrocytes of the coelomic fluid of certain invertebrates. It usually occurs as an octamer of molecular weight 108,000, in which each sub-unit contains two Fe atoms and reversibly binds one O2 molecule1. There is convincing evidence that myohaemerythrin, a monomeric protein found in the retractor muscles of the sipunculan worm Themiste pyroides, and the protomers of haemerythrin have quite similar tertiary structures2,...
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