On-line, continuous and automatic monitoring of extracellular malondialdehyde concentration in anesthetized rat brain cortex

An assay for in vivo, continuous and automatic monitoring of extracellular malondialdehyde concentrations in anesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion, on-line derivatization and on-line high-performance liquid chromatographic analysis. Microdialysate from an implanted microdialysis probe was on-line reacted with thiobarbituric acid at 80 degrees C for 10 min prior to on-line collection...