Minizymes. A new strategy for the development of reagentless amperometric biosensors based on direct electron-transfer processes

Direct electron transfer between an immobilized biological compound and an electrode is one of the most interesting transduction processes for the development of fast responding amperometric biosensors. Different biocatalysts like horseradish peroxidase, cytochrome c, myoglobin, microperoxidase MP-11 and haemin, all of them catalyzing the reduction of H2O2, have been investigated aiming on their ability for direct electron-transfer reactions...