l-Argininamide improves the refolding more effectively than l-arginine
Abstract
l-Arginine (Arg) is a widely used additive for suppressing protein aggregation during refolding. Systematic screening of Arg analogs provides superior additives that enhance the refolding yield more effectively than Arg. The refolding yield of hen egg lysozyme in the presence of 500 mM l-argininamide (ArgAd) increases 1.7-fold higher than Arg. Thermal unfolding experiments indicate that ArgAd has a greater denaturing effect than Arg. The...
Paper Details
Title
l-Argininamide improves the refolding more effectively than l-arginine
Published Date
Jun 1, 2007
Journal
Volume
130
Issue
2
Pages
153 - 160
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