The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
Abstract
Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye with affinity for hydrophobic parts of the protein, which are exposed as the protein unfolds. A simple fitting procedure allows quick calculation of the transition midpoint; the...
Paper Details
Title
The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
Published Date
Sep 1, 2007
Journal
Volume
2
Issue
9
Pages
2212 - 2221
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