RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Volume: 31, Issue: 3, Pages: 233 - 239
Published: Mar 1, 2013
Abstract
The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to sequences in vitro simply by modifying a short7 CRISPR RNA (crRNA) guide. Here we use this CRISPR-Cas system to introduce marker-free mutations in Streptococcus pneumoniae and Escherichia coli. The approach involves re-programming Cas9 by using a crRNA complementary to a...
Paper Details
Title
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Published Date
Mar 1, 2013
Volume
31
Issue
3
Pages
233 - 239
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