LC‐MS/MS‐based method for long‐term steroid profiling in human scalp hair

Summary Background Liquid chromatography–tandem mass spectrometry (LC‐MS/MS) is the method of choice for quantification of steroids. Human scalp hair provides the possibility to measure long‐term retrospective steroid concentrations, which is especially useful for steroids with large time‐dependent fluctuations in concentration, such as the glucocorticoid cortisol. Aim We set up and validated a LC‐MS/MS‐based method for long‐term steroid...