Metabolome and Proteome Profiling of Complex I Deficiency Induced by Rotenone

Complex I (CI; NADH dehydrogenase) deficiency causes mitochondrial diseases, including Leigh syndrome. A variety of clinical symptoms of CI deficiency are known, including neurodegeneration. Here, we report an integrative study combining liquid chromatography–mass spectrometry (LC–MS)-based metabolome and proteome profiling in CI deficient HeLa cells. We report a rapid LC–MS-based method for the relative quantification of targeted metabolome...