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Can we measure serum thyroglobulin

Published on May 1, 2002in Annals of Clinical Biochemistry1.893
· DOI :10.1258/0004563021902125
PM Clark1
Estimated H-index: 1
,
G Beckett1
Estimated H-index: 1
Abstract
Measurement of serum thyroglobulin (Tg) by immunoassay is used to monitor the treatment of differentiated thyroid cancer. An understanding of the disease process and the biochemistry of Tg allows the laboratory to determine the characteristics of suitable Tg assays. There are a number of key analytical issues: standardization, long-term assay stability, minimum detection limit, working range and the effect of endogenous Tg antibodies (TgAb). There are signiecant limitations to the methods used to determine TgAb interference. Clinicians should be aware of the limitations of immunoassays for this tumour marker. Ann Clin Biochem 2002; 39: 196-202
  • References (23)
  • Citations (28)
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References23
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#1Franco ArturiH-Index: 34
#2Diego RussoH-Index: 37
Last. Sebastiano FilettiH-Index: 58
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Objective: To investigate the molecular mechanisms underlying the influence of alteration of iodine trapping on the prognosis of metastatic papillary thyroid carcinomas, focusing on the expression of the Naa/Isymporter (NIS). Design: We evaluated the expression of the NIS gene in a series of 11 enlarged neck lymph-node metastases of papillary thyroid carcinomas, including four patients in whom an enlarged lymph node represented the first sign of the tumoral disease. Nine lymph nodes, either reac...
61 CitationsSource
Mortality rates from thyroid cancer have fallen significantly in recent decades, almost certainly as the result of earlier diagnosis and improved treatment of differentiated (papillary and follicular) thyroid cancer. Enhanced survival is likely a result of early diagnosis and therapy applied at a disease stage when treatment is most effective. In the United States and Europe, most patients at high risk for relapse and death from thyroid cancer are treated with total or near-total thyroidectomy a...
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#1Michele Marinò (UniPi: University of Pisa)H-Index: 31
#2Luca Chiovato (UniPi: University of Pisa)H-Index: 49
Last. Robert T. McCluskey (Harvard University)H-Index: 16
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After its endocytosis from the colloid, some thyroglobulin (Tg) is transcytosed intact across thyrocytes, accounting in part for its presence in the circulation. We previously showed that megalin (gp330), an endocytic Tg receptor, mediates apical to basolateral Tg transcytosis. Here we investigated whether a portion of megalin remains combined with Tg after its transcytosis, using studies with cultured thyroid cells and in vivo observations. FRTL-5 cells, a rat thyroid cell line, cultured on fil...
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#1Matthew D. Ringel (MedStar Washington Hospital Center)H-Index: 21
#2Pina L. Balducci-Silano (MedStar Washington Hospital Center)H-Index: 2
Last. R. Michael Tuttle (WRGH: Walter Reed Army Medical Center)H-Index: 4
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Patients with thyroid cancer are monitored for disease recurrence by measurement of serum thyroglobulin (Tg) and iodine-131 (131I) scanning. To enhance sensitivity and to circumvent antibodies that interfere with Tg immunoassays, we have developed RT-PCR assays that detect circulating thyroid messenger RNA (mRNA) transcripts. We now report results using a sensitive quantitative Tg mRNA assay (Taqman; ABI, Foster City, CA) in comparison with immunoassay in patients previously treated for thyroid ...
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#1P. HjiyiannakisH-Index: 1
#2J. MundyH-Index: 1
Last. C. HarmerH-Index: 1
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Abstract A retrospective review of patients with differentiated thyroid cancer (DTC) who were seen between 1984 and 1996 at the Royal Marsden Hospital identified 40 patients with serum thyroglobulin antibodies (TgAb). These antibodies can interfere with the immunoradiometric assay for serum thyroglobulin (Tg) used at this hospital, with resulting underestimation of the Tg level. A review of the case notes was carried out to ascertain the clinical significance of TgAb. The median follow-up from d...
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#1Susan T. WingoH-Index: 1
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Background: Thyroglobulin mRNA can be detected qualitatively in the peripheral blood of patients with metastatic thyroid cancer, thyroid cancer patients with residual thyroid bed uptake, and individuals with no known thyroid disease with intact thyroid glands by use of a lengthy, highly sensitive extraction technique. To improve and broaden the clinical usefulness of this assay, we developed a quantitative reverse transcription (RT)-PCR assay for thyroglobulin mRNA, using RNA recovered from whol...
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#1Maruyama MH-Index: 1
#2Kato RH-Index: 1
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#1Carole A. Spencer (SC: University of Southern California)H-Index: 47
#2M Takeuchi (SC: University of Southern California)H-Index: 4
Last. John T. Nicoloff (SC: University of Southern California)H-Index: 39
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The prevalence of circulating thyroid autoantibodies (TgAb or antithyroid peroxidase) was increased nearly 3-fold in patients with differentiated thyroid cancers (DTC) compared with the general population (40% vs. 14%, respectively). Serum TgAb (with or without antithyroid peroxidase) was present in 25% of DTC patients and 10% of the general population. Serial postsurgical serum TgAb and serum Tg patterns correlated with the presence or absence of disease. Measurements of serum Tg were made in 8...
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#1Peter Singer (SC: University of Southern California)H-Index: 79
#2David S. CooperH-Index: 55
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A set of minimum clinical guidelines for use by primary care physicians in the evaluation and management of patients with thyroid nodules or thyroid cancer was developed by consensus by an 11-member Standards of Care Committee (the authors of the article) of the American Thyroid Association, New York, NY. The participants were selected by the committee chairman and by the president of the American Thyroid Association based on their clinical experience. The committee members represented different...
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Cited By28
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#1Carole A. Spencer (SC: University of Southern California)H-Index: 47
#2Shireen Fatemi (KP: Kaiser Permanente)H-Index: 11
Thyroglobulin autoantibodies (TgAb) are detected at diagnosis or during treatment in approximately 25% of patients with differentiated thyroid cancer (DTC). When present, TgAb interferes with thyroglobulin (Tg) measurement causing falsely low or undetectable Tg immunometric assay (IMA) values that can mask disease. Guidelines mandate that every Tg test have TgAb measured simultaneously and quantitatively by immunoassay and not a recovery test. The propensity and magnitude of TgAb–Tg interference...
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#1Maomei Ruan (SJTU: Shanghai Jiao Tong University)H-Index: 3
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The present study was designed to investigate the association between response evaluation criteria in solid tumors (RECIST) 1.1 and 1.0, and to explore the utility of thyroglobulin (Tg) measurements in assessing tumor responses to sorafenib in patients with radioactive iodine (RAI)-refractory differentiated thyroid carcinoma (DTC). In total, 23 patients with RAI-refractory DTC were enrolled. A comparison of RECIST 1.1 and 1.0 was performed in all patients with measurable disease. Following the e...
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#1Carole A SpencerH-Index: 1
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#1Carole A. Spencer (SC: University of Southern California)H-Index: 47
#2Ivana Petrovic (SC: University of Southern California)H-Index: 2
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A comparison of four thyroglobulin autoantibody methods found that the use of manufacturer-recommended cut-offs was unreliable for detecting interfering thyroglobulin autoantibodies.
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#1Olgica B. Vrndic (University of Kragujevac)H-Index: 4
#2Svetlana SavinH-Index: 14
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Background: Measurement of serum thyroglobulin (Tg) is primarily used as a tumor marker in the postoperative management of patients with differentiated thyroid cancer (DTC), while thyroglobulin autoantibodies (TgAbs) are elevated in some patients as well. The aim of this study was to evaluate the concentrations of Tg and TgAbs in DTC patients 3 and 6 months after radioiodine therapy and to analyze whether the development and course of TgAbs is related to the clinical status of DTC patients or Tg...
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Last. Valentina Belardini (UNISI: University of Siena)H-Index: 4
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Nei pazienti affetti da carcinoma differenziato della tiroide (CDT), la rilevanza clinica ed economica di un follow-up ottimale, attraverso test diagnostici accurati, e una questione fondamentale, considerando il grande numero di pazienti nel mondo che vi si sottopongono per tutta la vita. I criteri di remissione a oggi utilizzati per i pazienti definiti a basso rischio di recidiva sono rappresentati da valori indosabili di tireoglobulina (Tg) stimolata associati a negativita dell’ecografia del ...
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Thyroid carcinomas are the most common malignant endocrine tumors. Thyroglobulin (Tg), a specific thyroid protein, is the most important tumor marker in thyroid oncology. After total thyroidectomy or radioiodine therapy, detectable or increasing serum Tg levels in patients with differentiated thyroid carcinoma indicate persistence of active thyroid tissue or cancer recurrence. Serum Tg concentration primarily reflects three variables: the mass of differentiated thyroid tissue present; the degree...
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#1Chih-Ching Huang (NTOU: National Taiwan Ocean University)H-Index: 46
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We have employed mannose-modified gold nanodots (Man–Au NDs) as a luminescence sensor for the detection of the thyroid-cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man–Au NDs are prepared through the reaction of 2.9 nm-diameter gold nanoparticles (Au NPs) with 11-mercapto-3,6,9-trioxaundecyl-α-D-mannopyranoside (Man-RSH) under the irradiation of a light-emitting diode (LED). We have found that the irradiation enhances the quantum yield (∼11 %), alters the emission w...
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#1Chao Ma (Qingdao University)H-Index: 3
#2Anren KuangH-Index: 4
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Background Differentiated thyroid carcinoma with thyroglobulin positive and radioactive iodine negative metastases has been observed in follow-up studies. The management of this condition remains controversial. Most studies support blind radioactive iodine treatment while others negate this approach. Objectives To assess the effects of radioiodine therapy for differentiated thyroid carcinoma with thyroglobulin positive and radioactive iodine negative metastases. Search methods Studies were obtai...
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Background: The measurement of serum thyroglobulin (Tg) is widely used as a marker for recurrence of thyroid carcinoma following total thyroidectomy. However, this method cannot differentiate between benign and malignant disease. We focused on the sugar chain in the Tg molecule and investigated the usefulness of Lens culinaris agglutinin (LCA)-reactive Tg ratios in sera and wash fluids obtained during fineneedle aspiration (FNA) for the detection of thyroid carcinoma. Methods: The study was perf...
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