Crystallization by Controlled Evaporation Leading to High Resolution Crystals of the C1 Domain of Cardiac Myosin Binding Protein-C (cMyBP-C)

This paper describes an optimization method for obtaining high quality crystals that has led to the successful structure determination of the C1 domain of cardiac myosin binding protein C. This method involved the controlled evaporation of hanging drops by induction of nucleation and its subsequent arrest. The crystals diffracted to a resolution of 1.5 Å in contrast to a maximum resolution of 3 Å when applying existing standard techniques. The...