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Two-step purification of tryptophan-accepting tRNA from Bacillus stearothermophilus☆

Published on Dec 1, 1985in Solid State Communications1.43
· DOI :10.1016/0003-2697(85)90213-1
Charles W. Carter7
Estimated H-index: 7
(UNC: University of North Carolina at Chapel Hill),
David C. Green2
Estimated H-index: 2
(UNC: University of North Carolina at Chapel Hill)
+ 1 AuthorsLaurie Betts18
Estimated H-index: 18
(UNC: University of North Carolina at Chapel Hill)
Abstract
Abstract Tryptophan-accepting tRNA has been purified essentially to homogeneity from Bacillus stearothermophilus . Crude tRNA was chromatographed first on benzoylated DEAE-cellulose and then on Sepharose 4B with reverse salt gradient elution. The product has tryptophan acceptor activity in excess of 2 nmol [ 14 C]tryptophan per A 260 unit. This procedure avoids costly aminoacylation, a step characteristic of other one- and two-step procedures. In two separate purifications 7 and 11 mg of tRNA trp were prepared from 750 and 1000 g of frozen cells, respectively. This yield compares favorably with that from other procedures. The pure tRNA trp has been crystallized under several different conditions.
  • References (8)
  • Citations (5)
References8
Newest
#1Charles W. Carter (UNC: University of North Carolina at Chapel Hill)H-Index: 33
#2David C. Green (UNC: University of North Carolina at Chapel Hill)H-Index: 2
#1Larry C. Waters (ORNL: Oak Ridge National Laboratory)H-Index: 19
#2Wen-Kuang YangH-Index: 4
Last.J. L. Nichols (ORNL: Oak Ridge National Laboratory)H-Index: 1
view all 4 authors...
#1S P ColowickH-Index: 1
#2M D JoshiH-Index: 1
Last.N O KaplanH-Index: 1
view all 4 authors...
Cited By5
Newest
#1Sylvie Doublié (UNC: University of North Carolina at Chapel Hill)H-Index: 30
#2Gérard Bricogne (LMB: Laboratory of Molecular Biology)H-Index: 39
Last.Charles W. Carter (UNC: University of North Carolina at Chapel Hill)H-Index: 33
view all 4 authors...
#1Hong Xue (U of T: University of Toronto)H-Index: 1
#2Wenyan Shen (U of T: University of Toronto)H-Index: 1
Last.J. Tze-Fei Wong (HKUST: Hong Kong University of Science and Technology)H-Index: 1
view all 3 authors...
#1Charles W. Carter (UNC: University of North Carolina at Chapel Hill)H-Index: 7
#2Eric T. Baldwin (UNC: University of North Carolina at Chapel Hill)H-Index: 1
Last.Lloyd Frick (UNC: University of North Carolina at Chapel Hill)H-Index: 7
view all 3 authors...
View next paperA deae-cellulose filter disk assay for aminoacyl-tRNA.