High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System inEscherichia coli

OG2 is a modified antimicrobial peptide, that is, derived from the frog peptide Palustrin-OG1. It has high antimicrobial activity and low cytotoxicity, and it is therefore promising as a therapeutic agent. Both prokaryotic ( Escherichia coli ) and eukaryotic ( Pichia pastoris ) production host systems were used to produce OG2 in our previous study; however, it was difficult to achieve high expression yields and efficient purification. In this...