A rapid method for assessing lipid:protein and detergent:protein ratios in membrane-protein crystallization
Volume: 59, Issue: 1, Pages: 77 - 83
Published: Dec 20, 2002
Abstract
A simple procedure for rapidly measuring lipid:protein ratios and detergent concentrations at different stages of the solubilization, purification and crystallization of membrane proteins has been developed. Fourier-transform infrared spectra recorded from 10 micro l aliquots of solution using a single-bounce diamond-attenuated total reflectance apparatus exhibit characteristic bands arising from the vibrations of lipid, protein and detergent....
Paper Details
Title
A rapid method for assessing lipid:protein and detergent:protein ratios in membrane-protein crystallization
Published Date
Dec 20, 2002
Volume
59
Issue
1
Pages
77 - 83
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