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Monitoring protein crystallization by dynamic light scattering

Published on Nov 20, 1989in FEBS Letters2.67
· DOI :10.1016/0014-5793(89)81616-3
Vincent Mikol7
Estimated H-index: 7
(CNRS: Centre national de la recherche scientifique),
Ernest Hirsch6
Estimated H-index: 6
,
Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique)
Abstract
Crystallization of lysozymes induced by temperature lowering has been monitored by dynamic light scattering from the onset of supersaturation to the growth of protein crystals up to a noticeable size (150–200 μm). The apparent size of the scatterers was found to increase up to a maximum value as supersaturation proceeded, then to decrease down to its initial value. Apparitions of crystals (20–30 μm) were observed during this decrease. Light scattering is thus proved to be a sensitive technique to follow protein crystallization and to provide useful information on the process.
  • References (11)
  • Citations (34)
References11
Newest
#1Charles W. Carter (UNC: University of North Carolina at Chapel Hill)H-Index: 7
#2Eric T. Baldwin (UNC: University of North Carolina at Chapel Hill)H-Index: 1
Last.Lloyd Frick (UNC: University of North Carolina at Chapel Hill)H-Index: 7
view all 3 authors...
#1Zvi Kam (UCSD: University of California, San Diego)H-Index: 41
#2H.B. Shore (SDSU: San Diego State University)H-Index: 1
Last.G. Feher (UCSD: University of California, San Diego)H-Index: 49
view all 3 authors...
Cited By34
Newest
#1Louis R. Nemzer (NSU: Nova Southeastern University)H-Index: 5
#2Bret N. Flanders (KSU: Kansas State University)H-Index: 14
Last.Christopher M. Sorensen (KSU: Kansas State University)H-Index: 52
view all 5 authors...
#1Kedar S. Deshpande (TU Delft: Delft University of Technology)H-Index: 3
#2Tangir Ahamed (TU Delft: Delft University of Technology)H-Index: 9
Last.Marcel Ottens (TU Delft: Delft University of Technology)H-Index: 20
view all 6 authors...
View next paperCharacterization of precrystallization aggregation of canavalin by dynamic light scattering.