Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii

Exploiting enzymes for industrial purposes often requires engineering of these enzymes to adapt them to the industrial requirements. In order to meet industrial demands, we improved the thermostability of endoglucanase Cel7B from Hypocrea pseudokoningii (HpCel7B), which was heterologously expressed in the yeast Pichia pastoris. Random mutants showing higher activity at elevated temperature have been selected and sequenced. In addition a model...